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In vivo study of the effects of exogenous hydrogen sulfide on lung mitochondria in acute lung injury in rats.

Du Q, Wang C, Zhang N, Li G, Zhang M, Li L, Zhang Q, Zhang J - BMC Anesthesiol (2014)

Bottom Line: Furthermore, mitochondrial activity, adenosine triphosphatease, superoxide dismutase, glutathione peroxidase, and mitochondrial Cyt-c protein expression were significantly decreased, and malondialdehyde content, mitochondrial swelling, and cytosol Cyt-c protein expression were significantly increased in the LPS injury group compared to the control group.These effects were lessened by NaHS.Its regulatory effect on lung mitochondria is positively correlated with the dosage.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Hebei Medical University, 361 Zhongshan Eastern Road, Shijiazhuang, 050017 China ; Intensive Care Unit, Hebei General Hospital, 348 Heping Western Road, Shijiazhuang, 050051 China.

ABSTRACT

Background: Acute lung injury (ALI) is a serious disease with high incidence in ICU, and impaired mitochondria function plays a significant role in ALI. In this study, we examined the possible roles of exogenous hydrogen sulfide (H2S) in lung mitochondria regulation in ALI rats.

Methods: The rat ALI model was induced by an intra-tongue vein Lipopolysaccharide (LPS) injection. We used sodium hydrosulphide (NaHS) as the H2S donor. We randomly divided 40 Sprague-Dawley rats into five groups: control, LPS injury, LPS + low-dose NaHS (0.78 mg • kg(-1)), LPS + middle-dose NaHS (1.56 mg • kg(-1)), and LPS + high-dose NaHS (3.12 mg • kg(-1)). Rats were killed 3 h after NaHS administration. We calculated a semi-quantitative histological index of lung injury assessments and measured the lung wet-to-dry weight ratio. We further analyzed serum for interleukin-1β levels using enzyme-linked immunosorbent assays. We observed lung mitochondria ultrastructures with an electron microscope. We examined oxidative stress markers in lung mitochondria and the mitochondrial swelling and activity. We analyzed lung mitochondria and cytosol Cyt-c protein expression using Western blotting.

Results: Compared to the control group, the quantitative assessment score index, wet-to-dry weight ratios, and interleukin-1β content in the LPS injury group were significantly increased and the mitochondrial ultrastructure damaged. Furthermore, mitochondrial activity, adenosine triphosphatease, superoxide dismutase, glutathione peroxidase, and mitochondrial Cyt-c protein expression were significantly decreased, and malondialdehyde content, mitochondrial swelling, and cytosol Cyt-c protein expression were significantly increased in the LPS injury group compared to the control group. These effects were lessened by NaHS.

Conclusion: Exogenous H2S provided a protective effect against ALI by decreasing the mitochondrial lipid peroxidation level and protecting the cell structure in the LPS-induced rat models. Its regulatory effect on lung mitochondria is positively correlated with the dosage.

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The expression of lung mitochondrial Cyt-c and cytosol Cyt-c were detected by Western blotting method. The Cyt-c protein expression of the lung mitochondria was significantly decreased in the LPS injury group compared with the control group (P < 0.01). In the LPS + low-dose, middle-dose and high-dose NaHS group, Cyt-c protein expression of the lung mitochondria was markedly increased compared with the LPS injury group (P < 0.05 or P < 0.01). The Cyt-c protein expression of cytosol was significantly increased in the LPS compared with the control group (P < 0.01). In the LPS + low-dose, middle-dose and high-dose NaHS group, Cyt-c protein expression of cytosol was markedly decreased compared with the LPS injury group (P < 0.05 or P < 0.01). a: control group. b: LPS injury group. c: LPS+ low-dose NaHS group. d: LPS + middle-dose NaHS group. e: LPS + high-dose NaHS group.
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Fig9: The expression of lung mitochondrial Cyt-c and cytosol Cyt-c were detected by Western blotting method. The Cyt-c protein expression of the lung mitochondria was significantly decreased in the LPS injury group compared with the control group (P < 0.01). In the LPS + low-dose, middle-dose and high-dose NaHS group, Cyt-c protein expression of the lung mitochondria was markedly increased compared with the LPS injury group (P < 0.05 or P < 0.01). The Cyt-c protein expression of cytosol was significantly increased in the LPS compared with the control group (P < 0.01). In the LPS + low-dose, middle-dose and high-dose NaHS group, Cyt-c protein expression of cytosol was markedly decreased compared with the LPS injury group (P < 0.05 or P < 0.01). a: control group. b: LPS injury group. c: LPS+ low-dose NaHS group. d: LPS + middle-dose NaHS group. e: LPS + high-dose NaHS group.

Mentions: The band intensity showed the Cyt-c protein expression. The Cyt-c protein expression in the lung mitochondria was significantly decreased in the LPS injury group compared to the control group (P < 0.01). In the three LPS + NaHS groups, Cyt-c protein expression in the lung mitochondria was markedly increased compared to the LPS injury group (P < 0.01). The cytosol Cyt-c protein expression was significantly increased in the LPS group compared to the control group (P < 0.01). In the three LPS + NaHS groups, cytosol Cyt-c protein expression was markedly decreased compared to the LPS injury group (P < 0.01) (Table 1, Figure 9).Table 1


In vivo study of the effects of exogenous hydrogen sulfide on lung mitochondria in acute lung injury in rats.

Du Q, Wang C, Zhang N, Li G, Zhang M, Li L, Zhang Q, Zhang J - BMC Anesthesiol (2014)

The expression of lung mitochondrial Cyt-c and cytosol Cyt-c were detected by Western blotting method. The Cyt-c protein expression of the lung mitochondria was significantly decreased in the LPS injury group compared with the control group (P < 0.01). In the LPS + low-dose, middle-dose and high-dose NaHS group, Cyt-c protein expression of the lung mitochondria was markedly increased compared with the LPS injury group (P < 0.05 or P < 0.01). The Cyt-c protein expression of cytosol was significantly increased in the LPS compared with the control group (P < 0.01). In the LPS + low-dose, middle-dose and high-dose NaHS group, Cyt-c protein expression of cytosol was markedly decreased compared with the LPS injury group (P < 0.05 or P < 0.01). a: control group. b: LPS injury group. c: LPS+ low-dose NaHS group. d: LPS + middle-dose NaHS group. e: LPS + high-dose NaHS group.
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Related In: Results  -  Collection

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Fig9: The expression of lung mitochondrial Cyt-c and cytosol Cyt-c were detected by Western blotting method. The Cyt-c protein expression of the lung mitochondria was significantly decreased in the LPS injury group compared with the control group (P < 0.01). In the LPS + low-dose, middle-dose and high-dose NaHS group, Cyt-c protein expression of the lung mitochondria was markedly increased compared with the LPS injury group (P < 0.05 or P < 0.01). The Cyt-c protein expression of cytosol was significantly increased in the LPS compared with the control group (P < 0.01). In the LPS + low-dose, middle-dose and high-dose NaHS group, Cyt-c protein expression of cytosol was markedly decreased compared with the LPS injury group (P < 0.05 or P < 0.01). a: control group. b: LPS injury group. c: LPS+ low-dose NaHS group. d: LPS + middle-dose NaHS group. e: LPS + high-dose NaHS group.
Mentions: The band intensity showed the Cyt-c protein expression. The Cyt-c protein expression in the lung mitochondria was significantly decreased in the LPS injury group compared to the control group (P < 0.01). In the three LPS + NaHS groups, Cyt-c protein expression in the lung mitochondria was markedly increased compared to the LPS injury group (P < 0.01). The cytosol Cyt-c protein expression was significantly increased in the LPS group compared to the control group (P < 0.01). In the three LPS + NaHS groups, cytosol Cyt-c protein expression was markedly decreased compared to the LPS injury group (P < 0.01) (Table 1, Figure 9).Table 1

Bottom Line: Furthermore, mitochondrial activity, adenosine triphosphatease, superoxide dismutase, glutathione peroxidase, and mitochondrial Cyt-c protein expression were significantly decreased, and malondialdehyde content, mitochondrial swelling, and cytosol Cyt-c protein expression were significantly increased in the LPS injury group compared to the control group.These effects were lessened by NaHS.Its regulatory effect on lung mitochondria is positively correlated with the dosage.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Hebei Medical University, 361 Zhongshan Eastern Road, Shijiazhuang, 050017 China ; Intensive Care Unit, Hebei General Hospital, 348 Heping Western Road, Shijiazhuang, 050051 China.

ABSTRACT

Background: Acute lung injury (ALI) is a serious disease with high incidence in ICU, and impaired mitochondria function plays a significant role in ALI. In this study, we examined the possible roles of exogenous hydrogen sulfide (H2S) in lung mitochondria regulation in ALI rats.

Methods: The rat ALI model was induced by an intra-tongue vein Lipopolysaccharide (LPS) injection. We used sodium hydrosulphide (NaHS) as the H2S donor. We randomly divided 40 Sprague-Dawley rats into five groups: control, LPS injury, LPS + low-dose NaHS (0.78 mg • kg(-1)), LPS + middle-dose NaHS (1.56 mg • kg(-1)), and LPS + high-dose NaHS (3.12 mg • kg(-1)). Rats were killed 3 h after NaHS administration. We calculated a semi-quantitative histological index of lung injury assessments and measured the lung wet-to-dry weight ratio. We further analyzed serum for interleukin-1β levels using enzyme-linked immunosorbent assays. We observed lung mitochondria ultrastructures with an electron microscope. We examined oxidative stress markers in lung mitochondria and the mitochondrial swelling and activity. We analyzed lung mitochondria and cytosol Cyt-c protein expression using Western blotting.

Results: Compared to the control group, the quantitative assessment score index, wet-to-dry weight ratios, and interleukin-1β content in the LPS injury group were significantly increased and the mitochondrial ultrastructure damaged. Furthermore, mitochondrial activity, adenosine triphosphatease, superoxide dismutase, glutathione peroxidase, and mitochondrial Cyt-c protein expression were significantly decreased, and malondialdehyde content, mitochondrial swelling, and cytosol Cyt-c protein expression were significantly increased in the LPS injury group compared to the control group. These effects were lessened by NaHS.

Conclusion: Exogenous H2S provided a protective effect against ALI by decreasing the mitochondrial lipid peroxidation level and protecting the cell structure in the LPS-induced rat models. Its regulatory effect on lung mitochondria is positively correlated with the dosage.

Show MeSH
Related in: MedlinePlus