Limits...
Prenatal development toxicity study of zinc oxide nanoparticles in rats.

Hong JS, Park MK, Kim MS, Lim JH, Park GJ, Maeng EH, Shin JH, Kim MK, Jeong J, Park JA, Kim JC, Shin HC - Int J Nanomedicine (2014)

Bottom Line: Toxicity in the dams manifested as significantly decreased body weight after administration of 400 mg/kg/day NPs; reduced food consumption after administration of 200 and 400 mg/kg/day NPs; and decreased liver weight and increased adrenal glands weight after administration of 400 mg/kg/day NPs.Meanwhile, no significant difference was found in the Zn content of fetal tissue between the control and high-dose groups.These results showed that oral doses for the study with 15-days repeated of ZnO(SM20(+)) NPs were maternotoxic in the 200 mg/kg/day group, and embryotoxic in the 400 mg/kg/day group.

View Article: PubMed Central - PubMed

Affiliation: Health Care Research Laboratory, Korea Testing and Research Institute, Gimpo, South Korea ; College of Veterinary Medicine, Konkuk University, Seoul, South Korea.

ABSTRACT
This study investigated the potential adverse effects of zinc oxide nanoparticles ([ZnO(SM20(+)) NPs] zinc oxide nanoparticles, positively charged, 20 nm) on pregnant dams and embryo-fetal development after maternal exposure over the period of gestational days 5-19 with Sprague-Dawley rats. ZnO(SM20(+)) NPs were administered to pregnant rats by gavage at 0, 100, 200, and 400 mg/kg/day. All dams were subjected to a cesarean section on gestational day 20, and all of the fetuses were examined for external, visceral, and skeletal alterations. Toxicity in the dams manifested as significantly decreased body weight after administration of 400 mg/kg/day NPs; reduced food consumption after administration of 200 and 400 mg/kg/day NPs; and decreased liver weight and increased adrenal glands weight after administration of 400 mg/kg/day NPs. However, no treatment-related difference in: number of corpora lutea; number of implantation sites; implantation rate (%); resorption; dead fetuses; litter size; fetal deaths and placental weights; and sex ratio were observed between the groups. On the other hand, significant decreases between treatment groups and controls were seen for fetal weights after administration of 400 mg/kg/day NPs. Morphological examinations of the fetuses demonstrated significant differences in incidences of abnormalities in the group administered 400mg/kg/day. Meanwhile, no significant difference was found in the Zn content of fetal tissue between the control and high-dose groups. These results showed that oral doses for the study with 15-days repeated of ZnO(SM20(+)) NPs were maternotoxic in the 200 mg/kg/day group, and embryotoxic in the 400 mg/kg/day group.

No MeSH data available.


Related in: MedlinePlus

Body weight changes of female rats during the gestation period.Notes: Pregnant rats were orally treated with ZnOSM20(+) nanoparticles for 15 days (G5–G19) with dose of 100, 200, and 400 mg/kg/day. “G” refers to the number of the days after gestation. “G0” refers to the day on which a rat becomes pregnant. Statistically different from the vehicle control group; *P<0.05.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4279776&req=5

f2-ijn-9-159: Body weight changes of female rats during the gestation period.Notes: Pregnant rats were orally treated with ZnOSM20(+) nanoparticles for 15 days (G5–G19) with dose of 100, 200, and 400 mg/kg/day. “G” refers to the number of the days after gestation. “G0” refers to the day on which a rat becomes pregnant. Statistically different from the vehicle control group; *P<0.05.

Mentions: Although seven of 24 dams from the 100-mg/kg/day group, 12 of 21 dams from the 200-mg/kg/day group, and 18 of 23 dams from the 400-mg/kg/day group showed salivation around the mouth in general appearance. Starting from 3–12 days after oral administration, alopecia (localized areas of partial alopecia) was observed in one pregnant rat from the 100-mg/kg/day group, one from the 200-mg/kg/day group, and one from the 400-mg/kg/day group (data not shown). This clinical sign was not recovered for the treatment period. The changes in body weight during the entire experimental period are listed in Figure 2. As shown by the data in Table 3, significant decreases in maternal body weight on GD 20 from the high-dose group was observed in comparison with the vehicle control group. The maternal-body-weight during pregnancy and corrected body weight were also significantly lower in the high-dose group than in the group. Statistically significant decreases in food consumption were noticed on day 18 of gestation in the 200- and 400-mg/kg/day groups in comparison to the vehicle control (Table 4). At the scheduled autopsy, one case of caveola of kidney surface in the vehicle control group; one case of splenomegaly in the 200-mg/kg/day group; and hypertrophy of adrenal and lung, edematous bowel, gastro-tympanites, and red reaction of liver in the 400-mg/kg/day group were observed in dams (data not shown). The absolute and relative organ weights of the pregnant rats treated with ZnOSM20(+) are presented in Table 5. Significantly decreased liver weight in the 400-mg/kg/day group was observed, and the increased absolute and relative weights of gland were significant in the 400-mg/kg/day group in dose-dependent manner in comparison with the control group.


Prenatal development toxicity study of zinc oxide nanoparticles in rats.

Hong JS, Park MK, Kim MS, Lim JH, Park GJ, Maeng EH, Shin JH, Kim MK, Jeong J, Park JA, Kim JC, Shin HC - Int J Nanomedicine (2014)

Body weight changes of female rats during the gestation period.Notes: Pregnant rats were orally treated with ZnOSM20(+) nanoparticles for 15 days (G5–G19) with dose of 100, 200, and 400 mg/kg/day. “G” refers to the number of the days after gestation. “G0” refers to the day on which a rat becomes pregnant. Statistically different from the vehicle control group; *P<0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4279776&req=5

f2-ijn-9-159: Body weight changes of female rats during the gestation period.Notes: Pregnant rats were orally treated with ZnOSM20(+) nanoparticles for 15 days (G5–G19) with dose of 100, 200, and 400 mg/kg/day. “G” refers to the number of the days after gestation. “G0” refers to the day on which a rat becomes pregnant. Statistically different from the vehicle control group; *P<0.05.
Mentions: Although seven of 24 dams from the 100-mg/kg/day group, 12 of 21 dams from the 200-mg/kg/day group, and 18 of 23 dams from the 400-mg/kg/day group showed salivation around the mouth in general appearance. Starting from 3–12 days after oral administration, alopecia (localized areas of partial alopecia) was observed in one pregnant rat from the 100-mg/kg/day group, one from the 200-mg/kg/day group, and one from the 400-mg/kg/day group (data not shown). This clinical sign was not recovered for the treatment period. The changes in body weight during the entire experimental period are listed in Figure 2. As shown by the data in Table 3, significant decreases in maternal body weight on GD 20 from the high-dose group was observed in comparison with the vehicle control group. The maternal-body-weight during pregnancy and corrected body weight were also significantly lower in the high-dose group than in the group. Statistically significant decreases in food consumption were noticed on day 18 of gestation in the 200- and 400-mg/kg/day groups in comparison to the vehicle control (Table 4). At the scheduled autopsy, one case of caveola of kidney surface in the vehicle control group; one case of splenomegaly in the 200-mg/kg/day group; and hypertrophy of adrenal and lung, edematous bowel, gastro-tympanites, and red reaction of liver in the 400-mg/kg/day group were observed in dams (data not shown). The absolute and relative organ weights of the pregnant rats treated with ZnOSM20(+) are presented in Table 5. Significantly decreased liver weight in the 400-mg/kg/day group was observed, and the increased absolute and relative weights of gland were significant in the 400-mg/kg/day group in dose-dependent manner in comparison with the control group.

Bottom Line: Toxicity in the dams manifested as significantly decreased body weight after administration of 400 mg/kg/day NPs; reduced food consumption after administration of 200 and 400 mg/kg/day NPs; and decreased liver weight and increased adrenal glands weight after administration of 400 mg/kg/day NPs.Meanwhile, no significant difference was found in the Zn content of fetal tissue between the control and high-dose groups.These results showed that oral doses for the study with 15-days repeated of ZnO(SM20(+)) NPs were maternotoxic in the 200 mg/kg/day group, and embryotoxic in the 400 mg/kg/day group.

View Article: PubMed Central - PubMed

Affiliation: Health Care Research Laboratory, Korea Testing and Research Institute, Gimpo, South Korea ; College of Veterinary Medicine, Konkuk University, Seoul, South Korea.

ABSTRACT
This study investigated the potential adverse effects of zinc oxide nanoparticles ([ZnO(SM20(+)) NPs] zinc oxide nanoparticles, positively charged, 20 nm) on pregnant dams and embryo-fetal development after maternal exposure over the period of gestational days 5-19 with Sprague-Dawley rats. ZnO(SM20(+)) NPs were administered to pregnant rats by gavage at 0, 100, 200, and 400 mg/kg/day. All dams were subjected to a cesarean section on gestational day 20, and all of the fetuses were examined for external, visceral, and skeletal alterations. Toxicity in the dams manifested as significantly decreased body weight after administration of 400 mg/kg/day NPs; reduced food consumption after administration of 200 and 400 mg/kg/day NPs; and decreased liver weight and increased adrenal glands weight after administration of 400 mg/kg/day NPs. However, no treatment-related difference in: number of corpora lutea; number of implantation sites; implantation rate (%); resorption; dead fetuses; litter size; fetal deaths and placental weights; and sex ratio were observed between the groups. On the other hand, significant decreases between treatment groups and controls were seen for fetal weights after administration of 400 mg/kg/day NPs. Morphological examinations of the fetuses demonstrated significant differences in incidences of abnormalities in the group administered 400mg/kg/day. Meanwhile, no significant difference was found in the Zn content of fetal tissue between the control and high-dose groups. These results showed that oral doses for the study with 15-days repeated of ZnO(SM20(+)) NPs were maternotoxic in the 200 mg/kg/day group, and embryotoxic in the 400 mg/kg/day group.

No MeSH data available.


Related in: MedlinePlus