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Prenatal development toxicity study of zinc oxide nanoparticles in rats.

Hong JS, Park MK, Kim MS, Lim JH, Park GJ, Maeng EH, Shin JH, Kim MK, Jeong J, Park JA, Kim JC, Shin HC - Int J Nanomedicine (2014)

Bottom Line: Toxicity in the dams manifested as significantly decreased body weight after administration of 400 mg/kg/day NPs; reduced food consumption after administration of 200 and 400 mg/kg/day NPs; and decreased liver weight and increased adrenal glands weight after administration of 400 mg/kg/day NPs.Meanwhile, no significant difference was found in the Zn content of fetal tissue between the control and high-dose groups.These results showed that oral doses for the study with 15-days repeated of ZnO(SM20(+)) NPs were maternotoxic in the 200 mg/kg/day group, and embryotoxic in the 400 mg/kg/day group.

View Article: PubMed Central - PubMed

Affiliation: Health Care Research Laboratory, Korea Testing and Research Institute, Gimpo, South Korea ; College of Veterinary Medicine, Konkuk University, Seoul, South Korea.

ABSTRACT
This study investigated the potential adverse effects of zinc oxide nanoparticles ([ZnO(SM20(+)) NPs] zinc oxide nanoparticles, positively charged, 20 nm) on pregnant dams and embryo-fetal development after maternal exposure over the period of gestational days 5-19 with Sprague-Dawley rats. ZnO(SM20(+)) NPs were administered to pregnant rats by gavage at 0, 100, 200, and 400 mg/kg/day. All dams were subjected to a cesarean section on gestational day 20, and all of the fetuses were examined for external, visceral, and skeletal alterations. Toxicity in the dams manifested as significantly decreased body weight after administration of 400 mg/kg/day NPs; reduced food consumption after administration of 200 and 400 mg/kg/day NPs; and decreased liver weight and increased adrenal glands weight after administration of 400 mg/kg/day NPs. However, no treatment-related difference in: number of corpora lutea; number of implantation sites; implantation rate (%); resorption; dead fetuses; litter size; fetal deaths and placental weights; and sex ratio were observed between the groups. On the other hand, significant decreases between treatment groups and controls were seen for fetal weights after administration of 400 mg/kg/day NPs. Morphological examinations of the fetuses demonstrated significant differences in incidences of abnormalities in the group administered 400mg/kg/day. Meanwhile, no significant difference was found in the Zn content of fetal tissue between the control and high-dose groups. These results showed that oral doses for the study with 15-days repeated of ZnO(SM20(+)) NPs were maternotoxic in the 200 mg/kg/day group, and embryotoxic in the 400 mg/kg/day group.

No MeSH data available.


Related in: MedlinePlus

The total Zn levels measured with ICP-AES.Notes: To investigate the placenta transfer of ZnOSM20(+) NPs in vivo, four extra female rats were used in the control (nontreatment control group; n=2) and 400-mg/kg/day (ZnOSM20(+) NPs; n=2) groups. Dosing occurred on gestational day 5–19 in the same manner as for main study animals.Abbreviations: ICP-AES, inductive coupled plasma atomic emission spectrometry; ZnOSM20(+) NPs, 20-nm positively charged ZnO nanoparticles.
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f1-ijn-9-159: The total Zn levels measured with ICP-AES.Notes: To investigate the placenta transfer of ZnOSM20(+) NPs in vivo, four extra female rats were used in the control (nontreatment control group; n=2) and 400-mg/kg/day (ZnOSM20(+) NPs; n=2) groups. Dosing occurred on gestational day 5–19 in the same manner as for main study animals.Abbreviations: ICP-AES, inductive coupled plasma atomic emission spectrometry; ZnOSM20(+) NPs, 20-nm positively charged ZnO nanoparticles.

Mentions: Zn concentrations in fetal tissues are shown in Figure 1. The measured total Zn levels by ICP-AES were 14.44±0.37 μg/g (mean ± standard deviation) for the control group and 16.47±2.19 μg/g Zn for the 400-mg/kg/day group (Table 2). The Zn contents in fetuses after in utero exposure to ZnOSM20(+) NPs was not significantly different from the Zn contents in control fetuses.


Prenatal development toxicity study of zinc oxide nanoparticles in rats.

Hong JS, Park MK, Kim MS, Lim JH, Park GJ, Maeng EH, Shin JH, Kim MK, Jeong J, Park JA, Kim JC, Shin HC - Int J Nanomedicine (2014)

The total Zn levels measured with ICP-AES.Notes: To investigate the placenta transfer of ZnOSM20(+) NPs in vivo, four extra female rats were used in the control (nontreatment control group; n=2) and 400-mg/kg/day (ZnOSM20(+) NPs; n=2) groups. Dosing occurred on gestational day 5–19 in the same manner as for main study animals.Abbreviations: ICP-AES, inductive coupled plasma atomic emission spectrometry; ZnOSM20(+) NPs, 20-nm positively charged ZnO nanoparticles.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4279776&req=5

f1-ijn-9-159: The total Zn levels measured with ICP-AES.Notes: To investigate the placenta transfer of ZnOSM20(+) NPs in vivo, four extra female rats were used in the control (nontreatment control group; n=2) and 400-mg/kg/day (ZnOSM20(+) NPs; n=2) groups. Dosing occurred on gestational day 5–19 in the same manner as for main study animals.Abbreviations: ICP-AES, inductive coupled plasma atomic emission spectrometry; ZnOSM20(+) NPs, 20-nm positively charged ZnO nanoparticles.
Mentions: Zn concentrations in fetal tissues are shown in Figure 1. The measured total Zn levels by ICP-AES were 14.44±0.37 μg/g (mean ± standard deviation) for the control group and 16.47±2.19 μg/g Zn for the 400-mg/kg/day group (Table 2). The Zn contents in fetuses after in utero exposure to ZnOSM20(+) NPs was not significantly different from the Zn contents in control fetuses.

Bottom Line: Toxicity in the dams manifested as significantly decreased body weight after administration of 400 mg/kg/day NPs; reduced food consumption after administration of 200 and 400 mg/kg/day NPs; and decreased liver weight and increased adrenal glands weight after administration of 400 mg/kg/day NPs.Meanwhile, no significant difference was found in the Zn content of fetal tissue between the control and high-dose groups.These results showed that oral doses for the study with 15-days repeated of ZnO(SM20(+)) NPs were maternotoxic in the 200 mg/kg/day group, and embryotoxic in the 400 mg/kg/day group.

View Article: PubMed Central - PubMed

Affiliation: Health Care Research Laboratory, Korea Testing and Research Institute, Gimpo, South Korea ; College of Veterinary Medicine, Konkuk University, Seoul, South Korea.

ABSTRACT
This study investigated the potential adverse effects of zinc oxide nanoparticles ([ZnO(SM20(+)) NPs] zinc oxide nanoparticles, positively charged, 20 nm) on pregnant dams and embryo-fetal development after maternal exposure over the period of gestational days 5-19 with Sprague-Dawley rats. ZnO(SM20(+)) NPs were administered to pregnant rats by gavage at 0, 100, 200, and 400 mg/kg/day. All dams were subjected to a cesarean section on gestational day 20, and all of the fetuses were examined for external, visceral, and skeletal alterations. Toxicity in the dams manifested as significantly decreased body weight after administration of 400 mg/kg/day NPs; reduced food consumption after administration of 200 and 400 mg/kg/day NPs; and decreased liver weight and increased adrenal glands weight after administration of 400 mg/kg/day NPs. However, no treatment-related difference in: number of corpora lutea; number of implantation sites; implantation rate (%); resorption; dead fetuses; litter size; fetal deaths and placental weights; and sex ratio were observed between the groups. On the other hand, significant decreases between treatment groups and controls were seen for fetal weights after administration of 400 mg/kg/day NPs. Morphological examinations of the fetuses demonstrated significant differences in incidences of abnormalities in the group administered 400mg/kg/day. Meanwhile, no significant difference was found in the Zn content of fetal tissue between the control and high-dose groups. These results showed that oral doses for the study with 15-days repeated of ZnO(SM20(+)) NPs were maternotoxic in the 200 mg/kg/day group, and embryotoxic in the 400 mg/kg/day group.

No MeSH data available.


Related in: MedlinePlus