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Effects of surface-modifying ligands on the colloidal stability of ZnO nanoparticle dispersions in in vitro cytotoxicity test media.

Kwon D, Park J, Park J, Choi SY, Yoon TH - Int J Nanomedicine (2014)

Bottom Line: The results showed that ZnO NPs were better dispersed in cell culture media via surface modification with positively or negatively charged molecules.Moreover, in the presence of fetal bovine serum (FBS) in RPMI and DMEM media, ZnO NPs were found even better dispersed for a longer period (at least 48 hours).This confirmed the important roles of surface-modifying compounds and of surface charge on the resultant cytotoxicities of NPs.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Nanoscale Characterization and Environmental Chemistry, Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul, South Korea.

ABSTRACT
The extrinsic physicochemical properties of nanoparticles (NPs), such as hydrodynamic size, surface charge, surface functional group, and colloidal stabilities, in toxicity testing media are known to have a significant influence on in vitro toxicity assessments. Therefore, interpretation of nanotoxicity test results should be based on reliable characterization of the NPs' extrinsic properties in actual toxicity testing media. Here, we present a set of physicochemical characterization results for commercially available ZnO NPs, including core diameter, hydrodynamic diameter, surface charges, and colloidal stabilities, in two in vitro toxicity testing media (Roswell Park Memorial Institute [RPMI] and Dulbecco's Modified Eagle's Medium [DMEM]), as well as simple cell viability assay results for selected ZnO NPs. Four commercially available and manufactured ZnO NPs, with different core sizes, were used in this study, and their surface charge was modified with five different surface coating materials (sodium citrate, tris(2-aminoethyl)amine, poly(acrylic acid), poly(allylamine hydrochloride), and poly-L-lysine hydrochloride). The results showed that ZnO NPs were better dispersed in cell culture media via surface modification with positively or negatively charged molecules. Moreover, in the presence of fetal bovine serum (FBS) in RPMI and DMEM media, ZnO NPs were found even better dispersed for a longer period (at least 48 hours). For the HeLa cells exposed to ZnO NPs in DMEM media without FBS, surface charge-dependent cytotoxicity trends were observed, while these trends were not observed for those cells cultured in FBS-containing media. This confirmed the important roles of surface-modifying compounds and of surface charge on the resultant cytotoxicities of NPs.

No MeSH data available.


Related in: MedlinePlus

Physicochemical characteristics of four commercial ZnO NPs in deionized water: (A) ZnOAA20, (B) ZnOSM20, (C) ZnOAE20, and (D) ZnOAE100.Notes: The top row shows TEM images; the middle row shows core diameter distribution from TEM images; the bottom row shows hydrodynamic diameter from DLS. Abbreviations: DLS, dynamic light scattering; TEM, transmission electron microscopy; NP, nanoparticle; ZnOAA20, 20 nm ZnO; ZnOAE20, 10–30 nm ZnO; ZnOAE100, <100 nm ZnO; ZnOSM20, 10–35 nm ZnO.
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f1-ijn-9-057: Physicochemical characteristics of four commercial ZnO NPs in deionized water: (A) ZnOAA20, (B) ZnOSM20, (C) ZnOAE20, and (D) ZnOAE100.Notes: The top row shows TEM images; the middle row shows core diameter distribution from TEM images; the bottom row shows hydrodynamic diameter from DLS. Abbreviations: DLS, dynamic light scattering; TEM, transmission electron microscopy; NP, nanoparticle; ZnOAA20, 20 nm ZnO; ZnOAE20, 10–30 nm ZnO; ZnOAE100, <100 nm ZnO; ZnOSM20, 10–35 nm ZnO.

Mentions: TEM was used to obtain information on the core diameter distributions and morphologies of the ZnO NPs (Figure 1). The core diameter distributions were estimated from image analysis of 500–1,000 ZnO NPs in representative TEM images (Figure 1). Most of the particles examined by TEM had spherical morphologies, and the core diameter distributions were in good agreement with manufacturer specifications, with the exception of the ZnOAE20 particles. In agreement with the manufacturer’s specification, the core diameters of ZnOAA20, ZnOSM20, and ZnOAE100 were measured as 22±7 nm, 20±9 nm, and 63±26 nm, respectively, while the ZnOAE20 (with manufacturer’s specification of 10–30 nm) was found to have much larger core diameter (57±25 nm) than that of the manufacturer’s specification, which might have been due to errors in the process of manufacturing, labeling, etc.


Effects of surface-modifying ligands on the colloidal stability of ZnO nanoparticle dispersions in in vitro cytotoxicity test media.

Kwon D, Park J, Park J, Choi SY, Yoon TH - Int J Nanomedicine (2014)

Physicochemical characteristics of four commercial ZnO NPs in deionized water: (A) ZnOAA20, (B) ZnOSM20, (C) ZnOAE20, and (D) ZnOAE100.Notes: The top row shows TEM images; the middle row shows core diameter distribution from TEM images; the bottom row shows hydrodynamic diameter from DLS. Abbreviations: DLS, dynamic light scattering; TEM, transmission electron microscopy; NP, nanoparticle; ZnOAA20, 20 nm ZnO; ZnOAE20, 10–30 nm ZnO; ZnOAE100, <100 nm ZnO; ZnOSM20, 10–35 nm ZnO.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4279775&req=5

f1-ijn-9-057: Physicochemical characteristics of four commercial ZnO NPs in deionized water: (A) ZnOAA20, (B) ZnOSM20, (C) ZnOAE20, and (D) ZnOAE100.Notes: The top row shows TEM images; the middle row shows core diameter distribution from TEM images; the bottom row shows hydrodynamic diameter from DLS. Abbreviations: DLS, dynamic light scattering; TEM, transmission electron microscopy; NP, nanoparticle; ZnOAA20, 20 nm ZnO; ZnOAE20, 10–30 nm ZnO; ZnOAE100, <100 nm ZnO; ZnOSM20, 10–35 nm ZnO.
Mentions: TEM was used to obtain information on the core diameter distributions and morphologies of the ZnO NPs (Figure 1). The core diameter distributions were estimated from image analysis of 500–1,000 ZnO NPs in representative TEM images (Figure 1). Most of the particles examined by TEM had spherical morphologies, and the core diameter distributions were in good agreement with manufacturer specifications, with the exception of the ZnOAE20 particles. In agreement with the manufacturer’s specification, the core diameters of ZnOAA20, ZnOSM20, and ZnOAE100 were measured as 22±7 nm, 20±9 nm, and 63±26 nm, respectively, while the ZnOAE20 (with manufacturer’s specification of 10–30 nm) was found to have much larger core diameter (57±25 nm) than that of the manufacturer’s specification, which might have been due to errors in the process of manufacturing, labeling, etc.

Bottom Line: The results showed that ZnO NPs were better dispersed in cell culture media via surface modification with positively or negatively charged molecules.Moreover, in the presence of fetal bovine serum (FBS) in RPMI and DMEM media, ZnO NPs were found even better dispersed for a longer period (at least 48 hours).This confirmed the important roles of surface-modifying compounds and of surface charge on the resultant cytotoxicities of NPs.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Nanoscale Characterization and Environmental Chemistry, Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul, South Korea.

ABSTRACT
The extrinsic physicochemical properties of nanoparticles (NPs), such as hydrodynamic size, surface charge, surface functional group, and colloidal stabilities, in toxicity testing media are known to have a significant influence on in vitro toxicity assessments. Therefore, interpretation of nanotoxicity test results should be based on reliable characterization of the NPs' extrinsic properties in actual toxicity testing media. Here, we present a set of physicochemical characterization results for commercially available ZnO NPs, including core diameter, hydrodynamic diameter, surface charges, and colloidal stabilities, in two in vitro toxicity testing media (Roswell Park Memorial Institute [RPMI] and Dulbecco's Modified Eagle's Medium [DMEM]), as well as simple cell viability assay results for selected ZnO NPs. Four commercially available and manufactured ZnO NPs, with different core sizes, were used in this study, and their surface charge was modified with five different surface coating materials (sodium citrate, tris(2-aminoethyl)amine, poly(acrylic acid), poly(allylamine hydrochloride), and poly-L-lysine hydrochloride). The results showed that ZnO NPs were better dispersed in cell culture media via surface modification with positively or negatively charged molecules. Moreover, in the presence of fetal bovine serum (FBS) in RPMI and DMEM media, ZnO NPs were found even better dispersed for a longer period (at least 48 hours). For the HeLa cells exposed to ZnO NPs in DMEM media without FBS, surface charge-dependent cytotoxicity trends were observed, while these trends were not observed for those cells cultured in FBS-containing media. This confirmed the important roles of surface-modifying compounds and of surface charge on the resultant cytotoxicities of NPs.

No MeSH data available.


Related in: MedlinePlus