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Toxicity of 100 nm zinc oxide nanoparticles: a report of 90-day repeated oral administration in Sprague Dawley rats.

Kim YR, Park JI, Lee EJ, Park SH, Seong NW, Kim JH, Kim GY, Meang EH, Hong JS, Kim SH, Koh SB, Kim MS, Kim CS, Kim SK, Son SW, Seo YR, Kang BH, Han BS, An SS, Yun HI, Kim MK - Int J Nanomedicine (2014)

Bottom Line: Nanoparticles (NPs) are used commercially in health and fitness fields, but information about the toxicity and mechanisms underlying the toxic effects of NPs is still very limited.Histopathological examination showed significant adverse effects (by both test articles) in the stomach, pancreas, eye, and prostate gland tissues, but the particle charge did not affect the tendency or the degree of the lesions.We speculate that this inflammatory damage might result from continuous irritation caused by both test articles.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Korea University Medical School and College, Seoul, Korea.

ABSTRACT
Nanoparticles (NPs) are used commercially in health and fitness fields, but information about the toxicity and mechanisms underlying the toxic effects of NPs is still very limited. The aim of this study is to investigate the toxic effect(s) of 100 nm negatively (ZnO(AE100[-])) or positively (ZnO(AE100[+])) charged zinc oxide (ZnO) NPs administered by gavage in Sprague Dawley rats, to establish a no observed adverse effect level, and to identify target organ(s). After verification of the primary particle size, morphology, hydrodynamic size, and zeta potential of each test article, we performed a 90-day study according to Organisation for Economic Co-operation and Development test guideline 408. For the 90-day study, the high dose was set at 500 mg/kg and the middle and low doses were set at 125 mg/kg and 31.25 mg/kg, respectively. Both ZnO NPs had significant changes in hematological and blood biochemical analysis, which could correlate with anemia-related parameters, in the 500 mg/kg groups of both sexes. Histopathological examination showed significant adverse effects (by both test articles) in the stomach, pancreas, eye, and prostate gland tissues, but the particle charge did not affect the tendency or the degree of the lesions. We speculate that this inflammatory damage might result from continuous irritation caused by both test articles. Therefore, the target organs for both ZnO(AE100(-)) and ZnO(AE100(+)) are considered to be the stomach, pancreas, eye, and prostate gland. Also, the no observed adverse effect level for both test articles was identified as 31.25 mg/kg for both sexes, because the adverse effects were observed at all doses greater than 125 mg/kg.

No MeSH data available.


Related in: MedlinePlus

Histopathological changes in the prostate gland after treatment with (A and B) ZnOAE100(−) and (C and D) ZnOAE100(+) at a dose of 500 mg/kg for 90 days. Prostate gland sections were stained with hematoxylin and eosin. (A and C) Control for the prostate gland tubule. (B and D) 500 mg/kg treatment groups.aNote:aArrows in (B and D) show tubular hyperplasia in the prostate gland.Abbreviations: A, suppurative inflammation, N, normal tubule; ZnO, zinc oxide; ZnOAE100(−), 100 nm negatively charged ZnO; ZnOAE100(+), 100 nm positively charged ZnO.
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f6-ijn-9-109: Histopathological changes in the prostate gland after treatment with (A and B) ZnOAE100(−) and (C and D) ZnOAE100(+) at a dose of 500 mg/kg for 90 days. Prostate gland sections were stained with hematoxylin and eosin. (A and C) Control for the prostate gland tubule. (B and D) 500 mg/kg treatment groups.aNote:aArrows in (B and D) show tubular hyperplasia in the prostate gland.Abbreviations: A, suppurative inflammation, N, normal tubule; ZnO, zinc oxide; ZnOAE100(−), 100 nm negatively charged ZnO; ZnOAE100(+), 100 nm positively charged ZnO.


Toxicity of 100 nm zinc oxide nanoparticles: a report of 90-day repeated oral administration in Sprague Dawley rats.

Kim YR, Park JI, Lee EJ, Park SH, Seong NW, Kim JH, Kim GY, Meang EH, Hong JS, Kim SH, Koh SB, Kim MS, Kim CS, Kim SK, Son SW, Seo YR, Kang BH, Han BS, An SS, Yun HI, Kim MK - Int J Nanomedicine (2014)

Histopathological changes in the prostate gland after treatment with (A and B) ZnOAE100(−) and (C and D) ZnOAE100(+) at a dose of 500 mg/kg for 90 days. Prostate gland sections were stained with hematoxylin and eosin. (A and C) Control for the prostate gland tubule. (B and D) 500 mg/kg treatment groups.aNote:aArrows in (B and D) show tubular hyperplasia in the prostate gland.Abbreviations: A, suppurative inflammation, N, normal tubule; ZnO, zinc oxide; ZnOAE100(−), 100 nm negatively charged ZnO; ZnOAE100(+), 100 nm positively charged ZnO.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4279774&req=5

f6-ijn-9-109: Histopathological changes in the prostate gland after treatment with (A and B) ZnOAE100(−) and (C and D) ZnOAE100(+) at a dose of 500 mg/kg for 90 days. Prostate gland sections were stained with hematoxylin and eosin. (A and C) Control for the prostate gland tubule. (B and D) 500 mg/kg treatment groups.aNote:aArrows in (B and D) show tubular hyperplasia in the prostate gland.Abbreviations: A, suppurative inflammation, N, normal tubule; ZnO, zinc oxide; ZnOAE100(−), 100 nm negatively charged ZnO; ZnOAE100(+), 100 nm positively charged ZnO.
Bottom Line: Nanoparticles (NPs) are used commercially in health and fitness fields, but information about the toxicity and mechanisms underlying the toxic effects of NPs is still very limited.Histopathological examination showed significant adverse effects (by both test articles) in the stomach, pancreas, eye, and prostate gland tissues, but the particle charge did not affect the tendency or the degree of the lesions.We speculate that this inflammatory damage might result from continuous irritation caused by both test articles.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Korea University Medical School and College, Seoul, Korea.

ABSTRACT
Nanoparticles (NPs) are used commercially in health and fitness fields, but information about the toxicity and mechanisms underlying the toxic effects of NPs is still very limited. The aim of this study is to investigate the toxic effect(s) of 100 nm negatively (ZnO(AE100[-])) or positively (ZnO(AE100[+])) charged zinc oxide (ZnO) NPs administered by gavage in Sprague Dawley rats, to establish a no observed adverse effect level, and to identify target organ(s). After verification of the primary particle size, morphology, hydrodynamic size, and zeta potential of each test article, we performed a 90-day study according to Organisation for Economic Co-operation and Development test guideline 408. For the 90-day study, the high dose was set at 500 mg/kg and the middle and low doses were set at 125 mg/kg and 31.25 mg/kg, respectively. Both ZnO NPs had significant changes in hematological and blood biochemical analysis, which could correlate with anemia-related parameters, in the 500 mg/kg groups of both sexes. Histopathological examination showed significant adverse effects (by both test articles) in the stomach, pancreas, eye, and prostate gland tissues, but the particle charge did not affect the tendency or the degree of the lesions. We speculate that this inflammatory damage might result from continuous irritation caused by both test articles. Therefore, the target organs for both ZnO(AE100(-)) and ZnO(AE100(+)) are considered to be the stomach, pancreas, eye, and prostate gland. Also, the no observed adverse effect level for both test articles was identified as 31.25 mg/kg for both sexes, because the adverse effects were observed at all doses greater than 125 mg/kg.

No MeSH data available.


Related in: MedlinePlus