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In vitro cytotoxicity of SiO2 or ZnO nanoparticles with different sizes and surface charges on U373MG human glioblastoma cells.

Kim JE, Kim H, An SS, Maeng EH, Kim MK, Song YJ - Int J Nanomedicine (2014)

Bottom Line: Silicon dioxide (SiO2) and zinc oxide (ZnO) nanoparticles are widely used in various applications, raising issues regarding the possible adverse effects of these metal oxide nanoparticles on human cells.The 20 nm SiO2 nanoparticles were more toxic than the 100 nm nanoparticles against U373MG cells, but the surface charge had little or no effect on their cytotoxicity.Both SiO2 and ZnO nanoparticles activated caspase-3 and induced DNA fragmentation in U373MG cells, suggesting the induction of apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, Gachon University, Seongnam-Si, South Korea.

ABSTRACT
Silicon dioxide (SiO2) and zinc oxide (ZnO) nanoparticles are widely used in various applications, raising issues regarding the possible adverse effects of these metal oxide nanoparticles on human cells. In this study, we determined the cytotoxic effects of differently charged SiO2 and ZnO nanoparticles, with mean sizes of either 100 or 20 nm, on the U373MG human glioblastoma cell line. The overall cytotoxicity of ZnO nanoparticles against U373MG cells was significantly higher than that of SiO2 nanoparticles. Neither the size nor the surface charge of the ZnO nanoparticles affected their cytotoxicity against U373MG cells. The 20 nm SiO2 nanoparticles were more toxic than the 100 nm nanoparticles against U373MG cells, but the surface charge had little or no effect on their cytotoxicity. Both SiO2 and ZnO nanoparticles activated caspase-3 and induced DNA fragmentation in U373MG cells, suggesting the induction of apoptosis. Thus, SiO2 and ZnO nanoparticles appear to exert cytotoxic effects against U373MG cells, possibly via apoptosis.

No MeSH data available.


Related in: MedlinePlus

The effect of SiO2 or ZnO NPs on DNA fragmentation.Notes: U373MG cells were treated with (A) SiO2 or (B) ZnO NPs with different sizes and surface charges at the concentrations described above. At 6 hours after treatment, fragmented DNA was labeled with fluorescein-12-UTP (green) and visualized under fluorescence microscopy. Nuclei were visualized by DAPI staining (blue).Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; NPs, nanoparticles; SiO2, silicon dioxide; ZnO, zinc oxide; UTP, uridine triphosphate.
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f6-ijn-9-235: The effect of SiO2 or ZnO NPs on DNA fragmentation.Notes: U373MG cells were treated with (A) SiO2 or (B) ZnO NPs with different sizes and surface charges at the concentrations described above. At 6 hours after treatment, fragmented DNA was labeled with fluorescein-12-UTP (green) and visualized under fluorescence microscopy. Nuclei were visualized by DAPI staining (blue).Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; NPs, nanoparticles; SiO2, silicon dioxide; ZnO, zinc oxide; UTP, uridine triphosphate.

Mentions: To further assess the apoptotic effects of SiO2 or ZnO NPs, U373MG cells were treated with the above-described NPs for 6 hours, and chromosomal DNA fragmentation was determined using a TUNEL assay. As expected, SiO2 NPs and ZnO NPs induced chromosomal DNA fragmentation in U373MG cells (Figure 6). At 6 hours after treatment with SiO2EN100(R), SiO2EN100(−), SiO2EN20(R), and SiO2EN20(−), 11.7%, 12.9%, 10.8%, and 10.1% of the cells were found to be TUNEL positive (Figure 6A). Compared to the SiO2 NPs, the ZnO NPs were more potent in inducing chromosomal DNA fragmentation as 42.7%, 60.4%, 42.8%, and 19.5% of the cells treated with ZnOAE100(+), ZnOAE100(−), ZnOSM20(+), and ZnOSM20(−), respectively, were found to be TUNEL positive (Figure 6B). These data suggest that the ZnO NPs may be more effective at inducing chromosomal DNA fragmentation in these cells (Figure 6B). In addition to the TUNEL assay, the comet assay was employed to examine DNA damage in SiO2 or ZnO NP-treated cells. Consistent with the TUNEL data, both SiO2 and ZnO NPs induced DNA damage in U373MG cells (data not shown). Taken together, these data indicate that both SiO2 and ZnO NPs reduce the viability of U373MG cells by inducing apoptosis, and further suggest that ZnO NPs may be more effective than SiO2 NPs for inducing apoptosis in U373MG cells.


In vitro cytotoxicity of SiO2 or ZnO nanoparticles with different sizes and surface charges on U373MG human glioblastoma cells.

Kim JE, Kim H, An SS, Maeng EH, Kim MK, Song YJ - Int J Nanomedicine (2014)

The effect of SiO2 or ZnO NPs on DNA fragmentation.Notes: U373MG cells were treated with (A) SiO2 or (B) ZnO NPs with different sizes and surface charges at the concentrations described above. At 6 hours after treatment, fragmented DNA was labeled with fluorescein-12-UTP (green) and visualized under fluorescence microscopy. Nuclei were visualized by DAPI staining (blue).Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; NPs, nanoparticles; SiO2, silicon dioxide; ZnO, zinc oxide; UTP, uridine triphosphate.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4279773&req=5

f6-ijn-9-235: The effect of SiO2 or ZnO NPs on DNA fragmentation.Notes: U373MG cells were treated with (A) SiO2 or (B) ZnO NPs with different sizes and surface charges at the concentrations described above. At 6 hours after treatment, fragmented DNA was labeled with fluorescein-12-UTP (green) and visualized under fluorescence microscopy. Nuclei were visualized by DAPI staining (blue).Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; NPs, nanoparticles; SiO2, silicon dioxide; ZnO, zinc oxide; UTP, uridine triphosphate.
Mentions: To further assess the apoptotic effects of SiO2 or ZnO NPs, U373MG cells were treated with the above-described NPs for 6 hours, and chromosomal DNA fragmentation was determined using a TUNEL assay. As expected, SiO2 NPs and ZnO NPs induced chromosomal DNA fragmentation in U373MG cells (Figure 6). At 6 hours after treatment with SiO2EN100(R), SiO2EN100(−), SiO2EN20(R), and SiO2EN20(−), 11.7%, 12.9%, 10.8%, and 10.1% of the cells were found to be TUNEL positive (Figure 6A). Compared to the SiO2 NPs, the ZnO NPs were more potent in inducing chromosomal DNA fragmentation as 42.7%, 60.4%, 42.8%, and 19.5% of the cells treated with ZnOAE100(+), ZnOAE100(−), ZnOSM20(+), and ZnOSM20(−), respectively, were found to be TUNEL positive (Figure 6B). These data suggest that the ZnO NPs may be more effective at inducing chromosomal DNA fragmentation in these cells (Figure 6B). In addition to the TUNEL assay, the comet assay was employed to examine DNA damage in SiO2 or ZnO NP-treated cells. Consistent with the TUNEL data, both SiO2 and ZnO NPs induced DNA damage in U373MG cells (data not shown). Taken together, these data indicate that both SiO2 and ZnO NPs reduce the viability of U373MG cells by inducing apoptosis, and further suggest that ZnO NPs may be more effective than SiO2 NPs for inducing apoptosis in U373MG cells.

Bottom Line: Silicon dioxide (SiO2) and zinc oxide (ZnO) nanoparticles are widely used in various applications, raising issues regarding the possible adverse effects of these metal oxide nanoparticles on human cells.The 20 nm SiO2 nanoparticles were more toxic than the 100 nm nanoparticles against U373MG cells, but the surface charge had little or no effect on their cytotoxicity.Both SiO2 and ZnO nanoparticles activated caspase-3 and induced DNA fragmentation in U373MG cells, suggesting the induction of apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, Gachon University, Seongnam-Si, South Korea.

ABSTRACT
Silicon dioxide (SiO2) and zinc oxide (ZnO) nanoparticles are widely used in various applications, raising issues regarding the possible adverse effects of these metal oxide nanoparticles on human cells. In this study, we determined the cytotoxic effects of differently charged SiO2 and ZnO nanoparticles, with mean sizes of either 100 or 20 nm, on the U373MG human glioblastoma cell line. The overall cytotoxicity of ZnO nanoparticles against U373MG cells was significantly higher than that of SiO2 nanoparticles. Neither the size nor the surface charge of the ZnO nanoparticles affected their cytotoxicity against U373MG cells. The 20 nm SiO2 nanoparticles were more toxic than the 100 nm nanoparticles against U373MG cells, but the surface charge had little or no effect on their cytotoxicity. Both SiO2 and ZnO nanoparticles activated caspase-3 and induced DNA fragmentation in U373MG cells, suggesting the induction of apoptosis. Thus, SiO2 and ZnO nanoparticles appear to exert cytotoxic effects against U373MG cells, possibly via apoptosis.

No MeSH data available.


Related in: MedlinePlus