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In vitro cytotoxicity of SiO2 or ZnO nanoparticles with different sizes and surface charges on U373MG human glioblastoma cells.

Kim JE, Kim H, An SS, Maeng EH, Kim MK, Song YJ - Int J Nanomedicine (2014)

Bottom Line: Silicon dioxide (SiO2) and zinc oxide (ZnO) nanoparticles are widely used in various applications, raising issues regarding the possible adverse effects of these metal oxide nanoparticles on human cells.The 20 nm SiO2 nanoparticles were more toxic than the 100 nm nanoparticles against U373MG cells, but the surface charge had little or no effect on their cytotoxicity.Both SiO2 and ZnO nanoparticles activated caspase-3 and induced DNA fragmentation in U373MG cells, suggesting the induction of apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, Gachon University, Seongnam-Si, South Korea.

ABSTRACT
Silicon dioxide (SiO2) and zinc oxide (ZnO) nanoparticles are widely used in various applications, raising issues regarding the possible adverse effects of these metal oxide nanoparticles on human cells. In this study, we determined the cytotoxic effects of differently charged SiO2 and ZnO nanoparticles, with mean sizes of either 100 or 20 nm, on the U373MG human glioblastoma cell line. The overall cytotoxicity of ZnO nanoparticles against U373MG cells was significantly higher than that of SiO2 nanoparticles. Neither the size nor the surface charge of the ZnO nanoparticles affected their cytotoxicity against U373MG cells. The 20 nm SiO2 nanoparticles were more toxic than the 100 nm nanoparticles against U373MG cells, but the surface charge had little or no effect on their cytotoxicity. Both SiO2 and ZnO nanoparticles activated caspase-3 and induced DNA fragmentation in U373MG cells, suggesting the induction of apoptosis. Thus, SiO2 and ZnO nanoparticles appear to exert cytotoxic effects against U373MG cells, possibly via apoptosis.

No MeSH data available.


Related in: MedlinePlus

The effect of ZnO nanoparticles on caspase activation.Notes: U373MG cells were treated with 20 μg/mL of (A) ZnOAE100(−), (B) ZnOSM20(−), (C) ZnOAE100(+), or (D) ZnOSM20(+) NPs. At 0, 1, 3, 6, 9 hours after treatment, PARP cleavage was determined by Western blot analysis.Abbreviations: NPs, nanoparticles; PARP, poly-(adenosine diphosphate-ribose) polymerase; ZnO, zinc oxide.
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f5-ijn-9-235: The effect of ZnO nanoparticles on caspase activation.Notes: U373MG cells were treated with 20 μg/mL of (A) ZnOAE100(−), (B) ZnOSM20(−), (C) ZnOAE100(+), or (D) ZnOSM20(+) NPs. At 0, 1, 3, 6, 9 hours after treatment, PARP cleavage was determined by Western blot analysis.Abbreviations: NPs, nanoparticles; PARP, poly-(adenosine diphosphate-ribose) polymerase; ZnO, zinc oxide.

Mentions: To determine whether SiO2 or ZnO NPs induce apoptosis, U373MG cells were treated with the above-described SiO2 or ZnO NPs, and caspase-3 activation was assessed by determining the proteolytic cleavage of PARP (from the native 116 kDa to 89 kDa) at 0, 1, 3, 6, and 9 hours after treatment. The treatment of U373MG cells with SiO2 NPs at the concentrations shown to reduce cell viability by 85% to 90% was found to rapidly induce PARP cleavage at 1 hour after treatment (Figure 4; compare lane 2 with lane 1). ZnOAE100(−) and ZnOSM20(−) also rapidly induced PARP cleavage in U373MG cells at 1 hour after treatment (Figure 5A and B; compare lane 2 with lane 1), whereas ZnOAE100(+) and ZnOSM20(+) induced PARP cleavage at later time points at 9 and 6 hour, respectively (Figure 5C and D; compare lane 2 with lane 1). These data indicate that both SiO2 and ZnO NPs induce caspase-3 activation, further suggesting that they both induce apoptosis.


In vitro cytotoxicity of SiO2 or ZnO nanoparticles with different sizes and surface charges on U373MG human glioblastoma cells.

Kim JE, Kim H, An SS, Maeng EH, Kim MK, Song YJ - Int J Nanomedicine (2014)

The effect of ZnO nanoparticles on caspase activation.Notes: U373MG cells were treated with 20 μg/mL of (A) ZnOAE100(−), (B) ZnOSM20(−), (C) ZnOAE100(+), or (D) ZnOSM20(+) NPs. At 0, 1, 3, 6, 9 hours after treatment, PARP cleavage was determined by Western blot analysis.Abbreviations: NPs, nanoparticles; PARP, poly-(adenosine diphosphate-ribose) polymerase; ZnO, zinc oxide.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4279773&req=5

f5-ijn-9-235: The effect of ZnO nanoparticles on caspase activation.Notes: U373MG cells were treated with 20 μg/mL of (A) ZnOAE100(−), (B) ZnOSM20(−), (C) ZnOAE100(+), or (D) ZnOSM20(+) NPs. At 0, 1, 3, 6, 9 hours after treatment, PARP cleavage was determined by Western blot analysis.Abbreviations: NPs, nanoparticles; PARP, poly-(adenosine diphosphate-ribose) polymerase; ZnO, zinc oxide.
Mentions: To determine whether SiO2 or ZnO NPs induce apoptosis, U373MG cells were treated with the above-described SiO2 or ZnO NPs, and caspase-3 activation was assessed by determining the proteolytic cleavage of PARP (from the native 116 kDa to 89 kDa) at 0, 1, 3, 6, and 9 hours after treatment. The treatment of U373MG cells with SiO2 NPs at the concentrations shown to reduce cell viability by 85% to 90% was found to rapidly induce PARP cleavage at 1 hour after treatment (Figure 4; compare lane 2 with lane 1). ZnOAE100(−) and ZnOSM20(−) also rapidly induced PARP cleavage in U373MG cells at 1 hour after treatment (Figure 5A and B; compare lane 2 with lane 1), whereas ZnOAE100(+) and ZnOSM20(+) induced PARP cleavage at later time points at 9 and 6 hour, respectively (Figure 5C and D; compare lane 2 with lane 1). These data indicate that both SiO2 and ZnO NPs induce caspase-3 activation, further suggesting that they both induce apoptosis.

Bottom Line: Silicon dioxide (SiO2) and zinc oxide (ZnO) nanoparticles are widely used in various applications, raising issues regarding the possible adverse effects of these metal oxide nanoparticles on human cells.The 20 nm SiO2 nanoparticles were more toxic than the 100 nm nanoparticles against U373MG cells, but the surface charge had little or no effect on their cytotoxicity.Both SiO2 and ZnO nanoparticles activated caspase-3 and induced DNA fragmentation in U373MG cells, suggesting the induction of apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, Gachon University, Seongnam-Si, South Korea.

ABSTRACT
Silicon dioxide (SiO2) and zinc oxide (ZnO) nanoparticles are widely used in various applications, raising issues regarding the possible adverse effects of these metal oxide nanoparticles on human cells. In this study, we determined the cytotoxic effects of differently charged SiO2 and ZnO nanoparticles, with mean sizes of either 100 or 20 nm, on the U373MG human glioblastoma cell line. The overall cytotoxicity of ZnO nanoparticles against U373MG cells was significantly higher than that of SiO2 nanoparticles. Neither the size nor the surface charge of the ZnO nanoparticles affected their cytotoxicity against U373MG cells. The 20 nm SiO2 nanoparticles were more toxic than the 100 nm nanoparticles against U373MG cells, but the surface charge had little or no effect on their cytotoxicity. Both SiO2 and ZnO nanoparticles activated caspase-3 and induced DNA fragmentation in U373MG cells, suggesting the induction of apoptosis. Thus, SiO2 and ZnO nanoparticles appear to exert cytotoxic effects against U373MG cells, possibly via apoptosis.

No MeSH data available.


Related in: MedlinePlus