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In vitro cytotoxicity of SiO2 or ZnO nanoparticles with different sizes and surface charges on U373MG human glioblastoma cells.

Kim JE, Kim H, An SS, Maeng EH, Kim MK, Song YJ - Int J Nanomedicine (2014)

Bottom Line: Silicon dioxide (SiO2) and zinc oxide (ZnO) nanoparticles are widely used in various applications, raising issues regarding the possible adverse effects of these metal oxide nanoparticles on human cells.The 20 nm SiO2 nanoparticles were more toxic than the 100 nm nanoparticles against U373MG cells, but the surface charge had little or no effect on their cytotoxicity.Both SiO2 and ZnO nanoparticles activated caspase-3 and induced DNA fragmentation in U373MG cells, suggesting the induction of apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, Gachon University, Seongnam-Si, South Korea.

ABSTRACT
Silicon dioxide (SiO2) and zinc oxide (ZnO) nanoparticles are widely used in various applications, raising issues regarding the possible adverse effects of these metal oxide nanoparticles on human cells. In this study, we determined the cytotoxic effects of differently charged SiO2 and ZnO nanoparticles, with mean sizes of either 100 or 20 nm, on the U373MG human glioblastoma cell line. The overall cytotoxicity of ZnO nanoparticles against U373MG cells was significantly higher than that of SiO2 nanoparticles. Neither the size nor the surface charge of the ZnO nanoparticles affected their cytotoxicity against U373MG cells. The 20 nm SiO2 nanoparticles were more toxic than the 100 nm nanoparticles against U373MG cells, but the surface charge had little or no effect on their cytotoxicity. Both SiO2 and ZnO nanoparticles activated caspase-3 and induced DNA fragmentation in U373MG cells, suggesting the induction of apoptosis. Thus, SiO2 and ZnO nanoparticles appear to exert cytotoxic effects against U373MG cells, possibly via apoptosis.

No MeSH data available.


Related in: MedlinePlus

The effect of ZnO NPs on the viability of U373MG cells.Notes: U373MG cells were treated with 0, 2, 5, 10, 15, or 20 μg/mL of (A) ZnOAE100(+), (B) ZnOAE100(−), (C) ZnOSM20(+), or (D) ZnOSM20(−) NPs. At 24 hours after treatment, cell viability was determined with the CellTiter-Glo assay. To calculate the relative luciferase activities, the luciferase activities at 0 hours after treatment were set to 100%. The data shown here represent the results from three independent experiments. Significant differences between samples were determined by the P-value of a two-sample t-test (P<0.05).Abbreviations: NPs, nanoparticles; RLU, relative luminescence units; ZnO, zinc oxide.
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f2-ijn-9-235: The effect of ZnO NPs on the viability of U373MG cells.Notes: U373MG cells were treated with 0, 2, 5, 10, 15, or 20 μg/mL of (A) ZnOAE100(+), (B) ZnOAE100(−), (C) ZnOSM20(+), or (D) ZnOSM20(−) NPs. At 24 hours after treatment, cell viability was determined with the CellTiter-Glo assay. To calculate the relative luciferase activities, the luciferase activities at 0 hours after treatment were set to 100%. The data shown here represent the results from three independent experiments. Significant differences between samples were determined by the P-value of a two-sample t-test (P<0.05).Abbreviations: NPs, nanoparticles; RLU, relative luminescence units; ZnO, zinc oxide.

Mentions: Compared to the SiO2 NPs, the ZnO NPs were significantly more toxic to U373MG cells as treatment with 20 μg/mL of ZnOAE100(+), ZnOAE100(−), ZnOSM20(+), and ZnOSM20(−) for 24 hours reduced the viability of U373MG cells by 53%, 47%, 74%, and 53%, respectively (Figure 2). The half- maximal inhibitory concentration values for the cytotoxicity of ZnOAE100(+), ZnOAE100(−), ZnOSM20(+), and ZnOSM20(−) on U373MG cells at 24 hours after treatment were 19.67, 20.47, 16.82, and 19.67 μg/mL, respectively (Table 1). Since treatment with 20 μg/mL of zinc chloride exhibited no cytotoxic effect against U373MG cells, the observed cytotoxicity appeared to have been due to the effect of the ZnO NPs (rather than Zn2+) on U373MG cells (Figure 3).


In vitro cytotoxicity of SiO2 or ZnO nanoparticles with different sizes and surface charges on U373MG human glioblastoma cells.

Kim JE, Kim H, An SS, Maeng EH, Kim MK, Song YJ - Int J Nanomedicine (2014)

The effect of ZnO NPs on the viability of U373MG cells.Notes: U373MG cells were treated with 0, 2, 5, 10, 15, or 20 μg/mL of (A) ZnOAE100(+), (B) ZnOAE100(−), (C) ZnOSM20(+), or (D) ZnOSM20(−) NPs. At 24 hours after treatment, cell viability was determined with the CellTiter-Glo assay. To calculate the relative luciferase activities, the luciferase activities at 0 hours after treatment were set to 100%. The data shown here represent the results from three independent experiments. Significant differences between samples were determined by the P-value of a two-sample t-test (P<0.05).Abbreviations: NPs, nanoparticles; RLU, relative luminescence units; ZnO, zinc oxide.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4279773&req=5

f2-ijn-9-235: The effect of ZnO NPs on the viability of U373MG cells.Notes: U373MG cells were treated with 0, 2, 5, 10, 15, or 20 μg/mL of (A) ZnOAE100(+), (B) ZnOAE100(−), (C) ZnOSM20(+), or (D) ZnOSM20(−) NPs. At 24 hours after treatment, cell viability was determined with the CellTiter-Glo assay. To calculate the relative luciferase activities, the luciferase activities at 0 hours after treatment were set to 100%. The data shown here represent the results from three independent experiments. Significant differences between samples were determined by the P-value of a two-sample t-test (P<0.05).Abbreviations: NPs, nanoparticles; RLU, relative luminescence units; ZnO, zinc oxide.
Mentions: Compared to the SiO2 NPs, the ZnO NPs were significantly more toxic to U373MG cells as treatment with 20 μg/mL of ZnOAE100(+), ZnOAE100(−), ZnOSM20(+), and ZnOSM20(−) for 24 hours reduced the viability of U373MG cells by 53%, 47%, 74%, and 53%, respectively (Figure 2). The half- maximal inhibitory concentration values for the cytotoxicity of ZnOAE100(+), ZnOAE100(−), ZnOSM20(+), and ZnOSM20(−) on U373MG cells at 24 hours after treatment were 19.67, 20.47, 16.82, and 19.67 μg/mL, respectively (Table 1). Since treatment with 20 μg/mL of zinc chloride exhibited no cytotoxic effect against U373MG cells, the observed cytotoxicity appeared to have been due to the effect of the ZnO NPs (rather than Zn2+) on U373MG cells (Figure 3).

Bottom Line: Silicon dioxide (SiO2) and zinc oxide (ZnO) nanoparticles are widely used in various applications, raising issues regarding the possible adverse effects of these metal oxide nanoparticles on human cells.The 20 nm SiO2 nanoparticles were more toxic than the 100 nm nanoparticles against U373MG cells, but the surface charge had little or no effect on their cytotoxicity.Both SiO2 and ZnO nanoparticles activated caspase-3 and induced DNA fragmentation in U373MG cells, suggesting the induction of apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, Gachon University, Seongnam-Si, South Korea.

ABSTRACT
Silicon dioxide (SiO2) and zinc oxide (ZnO) nanoparticles are widely used in various applications, raising issues regarding the possible adverse effects of these metal oxide nanoparticles on human cells. In this study, we determined the cytotoxic effects of differently charged SiO2 and ZnO nanoparticles, with mean sizes of either 100 or 20 nm, on the U373MG human glioblastoma cell line. The overall cytotoxicity of ZnO nanoparticles against U373MG cells was significantly higher than that of SiO2 nanoparticles. Neither the size nor the surface charge of the ZnO nanoparticles affected their cytotoxicity against U373MG cells. The 20 nm SiO2 nanoparticles were more toxic than the 100 nm nanoparticles against U373MG cells, but the surface charge had little or no effect on their cytotoxicity. Both SiO2 and ZnO nanoparticles activated caspase-3 and induced DNA fragmentation in U373MG cells, suggesting the induction of apoptosis. Thus, SiO2 and ZnO nanoparticles appear to exert cytotoxic effects against U373MG cells, possibly via apoptosis.

No MeSH data available.


Related in: MedlinePlus