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In vitro cytotoxicity of SiO2 or ZnO nanoparticles with different sizes and surface charges on U373MG human glioblastoma cells.

Kim JE, Kim H, An SS, Maeng EH, Kim MK, Song YJ - Int J Nanomedicine (2014)

Bottom Line: Silicon dioxide (SiO2) and zinc oxide (ZnO) nanoparticles are widely used in various applications, raising issues regarding the possible adverse effects of these metal oxide nanoparticles on human cells.The 20 nm SiO2 nanoparticles were more toxic than the 100 nm nanoparticles against U373MG cells, but the surface charge had little or no effect on their cytotoxicity.Both SiO2 and ZnO nanoparticles activated caspase-3 and induced DNA fragmentation in U373MG cells, suggesting the induction of apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, Gachon University, Seongnam-Si, South Korea.

ABSTRACT
Silicon dioxide (SiO2) and zinc oxide (ZnO) nanoparticles are widely used in various applications, raising issues regarding the possible adverse effects of these metal oxide nanoparticles on human cells. In this study, we determined the cytotoxic effects of differently charged SiO2 and ZnO nanoparticles, with mean sizes of either 100 or 20 nm, on the U373MG human glioblastoma cell line. The overall cytotoxicity of ZnO nanoparticles against U373MG cells was significantly higher than that of SiO2 nanoparticles. Neither the size nor the surface charge of the ZnO nanoparticles affected their cytotoxicity against U373MG cells. The 20 nm SiO2 nanoparticles were more toxic than the 100 nm nanoparticles against U373MG cells, but the surface charge had little or no effect on their cytotoxicity. Both SiO2 and ZnO nanoparticles activated caspase-3 and induced DNA fragmentation in U373MG cells, suggesting the induction of apoptosis. Thus, SiO2 and ZnO nanoparticles appear to exert cytotoxic effects against U373MG cells, possibly via apoptosis.

No MeSH data available.


Related in: MedlinePlus

The effect of SiO2 NPs on the viability of U373MG cells.Notes: U373MG cells were treated with various concentration of (A) SiO2EN100(R), (B) SiO2EN100(−), (C) SiO2EN20(R), or (D) SiO2EN20(−) NPs. At 24 hours after treatment, cell viability was determined with the CellTiter-Glo assay. To calculate the relative luciferase activities, the luciferase activities at 0 hours after treatment were set to 100%. The data shown here represent the results from three independent experiments.Abbreviations: NPs, nanoparticles; RLU, relative luminescence units; SiO2, silicon dioxide.
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f1-ijn-9-235: The effect of SiO2 NPs on the viability of U373MG cells.Notes: U373MG cells were treated with various concentration of (A) SiO2EN100(R), (B) SiO2EN100(−), (C) SiO2EN20(R), or (D) SiO2EN20(−) NPs. At 24 hours after treatment, cell viability was determined with the CellTiter-Glo assay. To calculate the relative luciferase activities, the luciferase activities at 0 hours after treatment were set to 100%. The data shown here represent the results from three independent experiments.Abbreviations: NPs, nanoparticles; RLU, relative luminescence units; SiO2, silicon dioxide.

Mentions: The U373MG cells were treated with various concentrations of SiO2 or ZnO NPs with different sizes and surface charges (ZnOAE100(+), ZnOAE100(−), ZnOSM20(+), ZnOSM20(−), SiO2EN100(R), SiO2EN100(−), SiO2EN20(R), and SiO2EN20(−)). After 24 hours, cell viability was measured using the CellTiter-Glo assay, which determines the presence of live and metabolically active cells by measuring adenosine triphosphate (Figure 1). Treatment with 6 mg/mL of SiO2EN100(R) and SiO2EN100(−) reduced the viability of U373MG cells by 68% and 65%, respectively (Figure 1A and B). Interestingly, the 20 nm SiO2 NPs were more toxic to U373MG cells than 100 nm SiO2 NPs. Treatment with 0.6 and 0.9 mg/mL of SiO2EN20(R) reduced the viability of U373MG cells by 90% and 98%, respectively (Figure 1C). Similarly, treatment with 0.6 and 0.8 mg/mL of SiO2EN20(−) reduced the viability of U373MG cells by 23% and 96%, respectively (Figure 1D). The cytotoxicity of SiO2 NPs was not cell-type specific as we observed similar levels of cytotoxicity against human dermal fibroblast and HCT116 human colorectal carcinoma cells (data not shown). The half-maximal inhibitory concentration values for the cytotoxicity of SiO2EN100(R), SiO2EN100(−), SiO2EN20(R) and SiO2EN20(−) against U373MG cells at 24 hours after treatment were 4.36, 4.93, 0.41, and 0.68 mg/mL, respectively (Table 1).


In vitro cytotoxicity of SiO2 or ZnO nanoparticles with different sizes and surface charges on U373MG human glioblastoma cells.

Kim JE, Kim H, An SS, Maeng EH, Kim MK, Song YJ - Int J Nanomedicine (2014)

The effect of SiO2 NPs on the viability of U373MG cells.Notes: U373MG cells were treated with various concentration of (A) SiO2EN100(R), (B) SiO2EN100(−), (C) SiO2EN20(R), or (D) SiO2EN20(−) NPs. At 24 hours after treatment, cell viability was determined with the CellTiter-Glo assay. To calculate the relative luciferase activities, the luciferase activities at 0 hours after treatment were set to 100%. The data shown here represent the results from three independent experiments.Abbreviations: NPs, nanoparticles; RLU, relative luminescence units; SiO2, silicon dioxide.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4279773&req=5

f1-ijn-9-235: The effect of SiO2 NPs on the viability of U373MG cells.Notes: U373MG cells were treated with various concentration of (A) SiO2EN100(R), (B) SiO2EN100(−), (C) SiO2EN20(R), or (D) SiO2EN20(−) NPs. At 24 hours after treatment, cell viability was determined with the CellTiter-Glo assay. To calculate the relative luciferase activities, the luciferase activities at 0 hours after treatment were set to 100%. The data shown here represent the results from three independent experiments.Abbreviations: NPs, nanoparticles; RLU, relative luminescence units; SiO2, silicon dioxide.
Mentions: The U373MG cells were treated with various concentrations of SiO2 or ZnO NPs with different sizes and surface charges (ZnOAE100(+), ZnOAE100(−), ZnOSM20(+), ZnOSM20(−), SiO2EN100(R), SiO2EN100(−), SiO2EN20(R), and SiO2EN20(−)). After 24 hours, cell viability was measured using the CellTiter-Glo assay, which determines the presence of live and metabolically active cells by measuring adenosine triphosphate (Figure 1). Treatment with 6 mg/mL of SiO2EN100(R) and SiO2EN100(−) reduced the viability of U373MG cells by 68% and 65%, respectively (Figure 1A and B). Interestingly, the 20 nm SiO2 NPs were more toxic to U373MG cells than 100 nm SiO2 NPs. Treatment with 0.6 and 0.9 mg/mL of SiO2EN20(R) reduced the viability of U373MG cells by 90% and 98%, respectively (Figure 1C). Similarly, treatment with 0.6 and 0.8 mg/mL of SiO2EN20(−) reduced the viability of U373MG cells by 23% and 96%, respectively (Figure 1D). The cytotoxicity of SiO2 NPs was not cell-type specific as we observed similar levels of cytotoxicity against human dermal fibroblast and HCT116 human colorectal carcinoma cells (data not shown). The half-maximal inhibitory concentration values for the cytotoxicity of SiO2EN100(R), SiO2EN100(−), SiO2EN20(R) and SiO2EN20(−) against U373MG cells at 24 hours after treatment were 4.36, 4.93, 0.41, and 0.68 mg/mL, respectively (Table 1).

Bottom Line: Silicon dioxide (SiO2) and zinc oxide (ZnO) nanoparticles are widely used in various applications, raising issues regarding the possible adverse effects of these metal oxide nanoparticles on human cells.The 20 nm SiO2 nanoparticles were more toxic than the 100 nm nanoparticles against U373MG cells, but the surface charge had little or no effect on their cytotoxicity.Both SiO2 and ZnO nanoparticles activated caspase-3 and induced DNA fragmentation in U373MG cells, suggesting the induction of apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, Gachon University, Seongnam-Si, South Korea.

ABSTRACT
Silicon dioxide (SiO2) and zinc oxide (ZnO) nanoparticles are widely used in various applications, raising issues regarding the possible adverse effects of these metal oxide nanoparticles on human cells. In this study, we determined the cytotoxic effects of differently charged SiO2 and ZnO nanoparticles, with mean sizes of either 100 or 20 nm, on the U373MG human glioblastoma cell line. The overall cytotoxicity of ZnO nanoparticles against U373MG cells was significantly higher than that of SiO2 nanoparticles. Neither the size nor the surface charge of the ZnO nanoparticles affected their cytotoxicity against U373MG cells. The 20 nm SiO2 nanoparticles were more toxic than the 100 nm nanoparticles against U373MG cells, but the surface charge had little or no effect on their cytotoxicity. Both SiO2 and ZnO nanoparticles activated caspase-3 and induced DNA fragmentation in U373MG cells, suggesting the induction of apoptosis. Thus, SiO2 and ZnO nanoparticles appear to exert cytotoxic effects against U373MG cells, possibly via apoptosis.

No MeSH data available.


Related in: MedlinePlus