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Toxicity of colloidal silica nanoparticles administered orally for 90 days in rats.

Kim YR, Lee SY, Lee EJ, Park SH, Seong NW, Seo HS, Shin SS, Kim SJ, Meang EH, Park MK, Kim MS, Kim CS, Kim SK, Son SW, Seo YR, Kang BH, Han BS, An SS, Lee BJ, Kim MK - Int J Nanomedicine (2014)

Bottom Line: Based on the results of the 14-day dose range-finding study, a high dose was determined to be 2,000 mg/kg, and middle and low doses were set at 1,000 and 500 mg/kg, respectively.In addition, no treatment-related clinical changes or histopathological findings were observed in any of the experimental groups.Moreover, no difference in toxic effects from chronic exposure to SiO2 (EN20(-))(20 nm) or SiO2 (EN100(-)) (100 nm) was observed.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Korea University Medical School and College, Seoul, Republic of Korea.

ABSTRACT
This study was undertaken to investigate the potential toxicity and establish the no observed adverse effect level (NOAEL) and target organ(s) of negatively charged colloidal silica particles of different sizes, ie, SiO2 (EN20(-)) (20 nm) or SiO2 (EN100(-)) 2(100 nm), administered by gavage in Sprague-Dawley rats. After verification of the physicochemical properties of the SiO2 particles to be tested, a preliminary dose range-finding study and 90-day repeated dose study were conducted according to the Organisation for Economic Cooperation and Development test guideline. Based on the results of the 14-day dose range-finding study, a high dose was determined to be 2,000 mg/kg, and middle and low doses were set at 1,000 and 500 mg/kg, respectively. In the 90-day toxicity study, there were no animal deaths in relation to administration of SiO2 particles of either size. In addition, no treatment-related clinical changes or histopathological findings were observed in any of the experimental groups. Moreover, no difference in toxic effects from chronic exposure to SiO2 (EN20(-))(20 nm) or SiO2 (EN100(-)) (100 nm) was observed. The results of this study indicate that the NOAEL for SiO2 (EN20(-)) and SiO2 (EN100(-)) would most likely be 2,000 mg/kg, and no target organ was identified in rats of either sex.

No MeSH data available.


Related in: MedlinePlus

Growth curves for (A) male and (B) female rats administered 100 nm SiO2 nanoparticles (SiO2EN100(−)) by gavage for 90 days.Notes: SiO2EN100(−) was orally administered to Sprague-Dawley rats at doses of 500, 1,000, and 2,000 mg/kg for 90 days. Body weight was measured and recorded once per week after the start of treatment during the study period. The results are presented as the mean ± standard deviation.Abbreviations: G1, control; G2, 500 mg/kg treatment group; G3, 1,000 mg/kg treatment group; and G4, 2,000 mg/kg treatment group.
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f2-ijn-9-067: Growth curves for (A) male and (B) female rats administered 100 nm SiO2 nanoparticles (SiO2EN100(−)) by gavage for 90 days.Notes: SiO2EN100(−) was orally administered to Sprague-Dawley rats at doses of 500, 1,000, and 2,000 mg/kg for 90 days. Body weight was measured and recorded once per week after the start of treatment during the study period. The results are presented as the mean ± standard deviation.Abbreviations: G1, control; G2, 500 mg/kg treatment group; G3, 1,000 mg/kg treatment group; and G4, 2,000 mg/kg treatment group.

Mentions: Regardless of SiO2 particle size, there were no statistically significant differences in body weight between treated rats and their respective control groups, for either males or females (Figures 1 and 2).


Toxicity of colloidal silica nanoparticles administered orally for 90 days in rats.

Kim YR, Lee SY, Lee EJ, Park SH, Seong NW, Seo HS, Shin SS, Kim SJ, Meang EH, Park MK, Kim MS, Kim CS, Kim SK, Son SW, Seo YR, Kang BH, Han BS, An SS, Lee BJ, Kim MK - Int J Nanomedicine (2014)

Growth curves for (A) male and (B) female rats administered 100 nm SiO2 nanoparticles (SiO2EN100(−)) by gavage for 90 days.Notes: SiO2EN100(−) was orally administered to Sprague-Dawley rats at doses of 500, 1,000, and 2,000 mg/kg for 90 days. Body weight was measured and recorded once per week after the start of treatment during the study period. The results are presented as the mean ± standard deviation.Abbreviations: G1, control; G2, 500 mg/kg treatment group; G3, 1,000 mg/kg treatment group; and G4, 2,000 mg/kg treatment group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4279771&req=5

f2-ijn-9-067: Growth curves for (A) male and (B) female rats administered 100 nm SiO2 nanoparticles (SiO2EN100(−)) by gavage for 90 days.Notes: SiO2EN100(−) was orally administered to Sprague-Dawley rats at doses of 500, 1,000, and 2,000 mg/kg for 90 days. Body weight was measured and recorded once per week after the start of treatment during the study period. The results are presented as the mean ± standard deviation.Abbreviations: G1, control; G2, 500 mg/kg treatment group; G3, 1,000 mg/kg treatment group; and G4, 2,000 mg/kg treatment group.
Mentions: Regardless of SiO2 particle size, there were no statistically significant differences in body weight between treated rats and their respective control groups, for either males or females (Figures 1 and 2).

Bottom Line: Based on the results of the 14-day dose range-finding study, a high dose was determined to be 2,000 mg/kg, and middle and low doses were set at 1,000 and 500 mg/kg, respectively.In addition, no treatment-related clinical changes or histopathological findings were observed in any of the experimental groups.Moreover, no difference in toxic effects from chronic exposure to SiO2 (EN20(-))(20 nm) or SiO2 (EN100(-)) (100 nm) was observed.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Korea University Medical School and College, Seoul, Republic of Korea.

ABSTRACT
This study was undertaken to investigate the potential toxicity and establish the no observed adverse effect level (NOAEL) and target organ(s) of negatively charged colloidal silica particles of different sizes, ie, SiO2 (EN20(-)) (20 nm) or SiO2 (EN100(-)) 2(100 nm), administered by gavage in Sprague-Dawley rats. After verification of the physicochemical properties of the SiO2 particles to be tested, a preliminary dose range-finding study and 90-day repeated dose study were conducted according to the Organisation for Economic Cooperation and Development test guideline. Based on the results of the 14-day dose range-finding study, a high dose was determined to be 2,000 mg/kg, and middle and low doses were set at 1,000 and 500 mg/kg, respectively. In the 90-day toxicity study, there were no animal deaths in relation to administration of SiO2 particles of either size. In addition, no treatment-related clinical changes or histopathological findings were observed in any of the experimental groups. Moreover, no difference in toxic effects from chronic exposure to SiO2 (EN20(-))(20 nm) or SiO2 (EN100(-)) (100 nm) was observed. The results of this study indicate that the NOAEL for SiO2 (EN20(-)) and SiO2 (EN100(-)) would most likely be 2,000 mg/kg, and no target organ was identified in rats of either sex.

No MeSH data available.


Related in: MedlinePlus