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Analysis of SiO2 nanoparticles binding proteins in rat blood and brain homogenate.

Shim KH, Hulme J, Maeng EH, Kim MK, An SS - Int J Nanomedicine (2014)

Bottom Line: In total, 115 and 48 plasma proteins from the rat were identified as being bound to negatively charged 20 nm and 100 nm SiO2 nanoparticles, respectively, and 50 and 36 proteins were found for 20 nm and 100 nm arginine-coated SiO2 nanoparticles, respectively.When proteins were compared between the two charges, higher numbers of proteins were found for arginine-coated positively charged SiO2 nanoparticles than for the negatively charged nanoparticles.Proteins bound on the surface of nanoparticles may affect functional and conformational properties and distributions in complicated biological processes.

View Article: PubMed Central - PubMed

Affiliation: Department of Bionano Technology, Gachon Medical Research Institute, Gachon University, Sungnam-si, South Korea.

ABSTRACT
A multitude of nanoparticles, such as titanium oxide (TiO2), zinc oxide, aluminum oxide, gold oxide, silver oxide, iron oxide, and silica oxide, are found in many chemical, cosmetic, pharmaceutical, and electronic products. Recently, SiO2 nanoparticles were shown to have an inert toxicity profile and no association with an irreversible toxicological change in animal models. Hence, exposure to SiO2 nanoparticles is on the increase. SiO2 nanoparticles are routinely used in numerous materials, from strengthening filler for concrete and other construction composites, to nontoxic platforms for biomedical application, such as drug delivery and theragnostics. On the other hand, recent in vitro experiments indicated that SiO2 nanoparticles were cytotoxic. Therefore, we investigated these nanoparticles to identify potentially toxic pathways by analyzing the adsorbed protein corona on the surface of SiO2 nanoparticles in the blood and brain of the rat. Four types of SiO2 nanoparticles were chosen for investigation, and the protein corona of each type was analyzed using liquid chromatography-tandem mass spectrometry technology. In total, 115 and 48 plasma proteins from the rat were identified as being bound to negatively charged 20 nm and 100 nm SiO2 nanoparticles, respectively, and 50 and 36 proteins were found for 20 nm and 100 nm arginine-coated SiO2 nanoparticles, respectively. Higher numbers of proteins were adsorbed onto the 20 nm sized SiO2 nanoparticles than onto the 100 nm sized nanoparticles regardless of charge. When proteins were compared between the two charges, higher numbers of proteins were found for arginine-coated positively charged SiO2 nanoparticles than for the negatively charged nanoparticles. The proteins identified as bound in the corona from SiO2 nanoparticles were further analyzed with ClueGO, a Cytoscape plugin used in protein ontology and for identifying biological interaction pathways. Proteins bound on the surface of nanoparticles may affect functional and conformational properties and distributions in complicated biological processes.

No MeSH data available.


Related in: MedlinePlus

Visualized biological processes associated with binding of proteins from plasma and brain homogenate with SiO2 nanoparticles. (A) Plasma and positively charged 20 mm SiO2 nanoparticles, (B) plasma and positively charged 100 mm SiO2 nanoparticles, (C) brain homogenate and negatively charged 20 nm SiO2 nanoparticles, and (D) brain homogenate and positively charged 100 mm SiO2 nanoparticles.Abbreviations: BH, brain homogenate; ADP, adenosine diphosphate; RNA, ribonucleic acid.
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f2-ijn-9-207: Visualized biological processes associated with binding of proteins from plasma and brain homogenate with SiO2 nanoparticles. (A) Plasma and positively charged 20 mm SiO2 nanoparticles, (B) plasma and positively charged 100 mm SiO2 nanoparticles, (C) brain homogenate and negatively charged 20 nm SiO2 nanoparticles, and (D) brain homogenate and positively charged 100 mm SiO2 nanoparticles.Abbreviations: BH, brain homogenate; ADP, adenosine diphosphate; RNA, ribonucleic acid.

Mentions: As with SiO2EN20(−), proteins involved in the acute inflammatory response bound to SiO2EN20(R), including proteins from glycolysis, protein polymerization, and the biosynthetic process for adenosine diphosphate (Figure 2). In brain homogenate, SiO2EN20(R) bound with proteins from endocytosis and protein folding, as in case with SiO2EN20(−); moreover, proteins involved in cell morphogenesis, including those for differentiation, biosynthesis of adenosine diphosphate, glucose catabolism, regulation of axon diameter, RNA splicing, amino acid metabolism, and microtubule-based movement, were also found. The plasma protein results for SiO2EN20(R) were similar to those for SiO2EN100(−) with proteins involved in blood coagulation and the acute inflammatory response being found in the protein corona. In SiO2EN20(R), similar proteins of brain homogenate from protein folding, RNA splicing, and negative regulation of microtubule depolymerization were found in the protein corona, in addition to proteins involved in the metabolism of cellular polysaccharides, the biosynthetic process for adenosine diphosphate, protein polymerization, glucose catabolism, and amino acid metabolism.


Analysis of SiO2 nanoparticles binding proteins in rat blood and brain homogenate.

Shim KH, Hulme J, Maeng EH, Kim MK, An SS - Int J Nanomedicine (2014)

Visualized biological processes associated with binding of proteins from plasma and brain homogenate with SiO2 nanoparticles. (A) Plasma and positively charged 20 mm SiO2 nanoparticles, (B) plasma and positively charged 100 mm SiO2 nanoparticles, (C) brain homogenate and negatively charged 20 nm SiO2 nanoparticles, and (D) brain homogenate and positively charged 100 mm SiO2 nanoparticles.Abbreviations: BH, brain homogenate; ADP, adenosine diphosphate; RNA, ribonucleic acid.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4279766&req=5

f2-ijn-9-207: Visualized biological processes associated with binding of proteins from plasma and brain homogenate with SiO2 nanoparticles. (A) Plasma and positively charged 20 mm SiO2 nanoparticles, (B) plasma and positively charged 100 mm SiO2 nanoparticles, (C) brain homogenate and negatively charged 20 nm SiO2 nanoparticles, and (D) brain homogenate and positively charged 100 mm SiO2 nanoparticles.Abbreviations: BH, brain homogenate; ADP, adenosine diphosphate; RNA, ribonucleic acid.
Mentions: As with SiO2EN20(−), proteins involved in the acute inflammatory response bound to SiO2EN20(R), including proteins from glycolysis, protein polymerization, and the biosynthetic process for adenosine diphosphate (Figure 2). In brain homogenate, SiO2EN20(R) bound with proteins from endocytosis and protein folding, as in case with SiO2EN20(−); moreover, proteins involved in cell morphogenesis, including those for differentiation, biosynthesis of adenosine diphosphate, glucose catabolism, regulation of axon diameter, RNA splicing, amino acid metabolism, and microtubule-based movement, were also found. The plasma protein results for SiO2EN20(R) were similar to those for SiO2EN100(−) with proteins involved in blood coagulation and the acute inflammatory response being found in the protein corona. In SiO2EN20(R), similar proteins of brain homogenate from protein folding, RNA splicing, and negative regulation of microtubule depolymerization were found in the protein corona, in addition to proteins involved in the metabolism of cellular polysaccharides, the biosynthetic process for adenosine diphosphate, protein polymerization, glucose catabolism, and amino acid metabolism.

Bottom Line: In total, 115 and 48 plasma proteins from the rat were identified as being bound to negatively charged 20 nm and 100 nm SiO2 nanoparticles, respectively, and 50 and 36 proteins were found for 20 nm and 100 nm arginine-coated SiO2 nanoparticles, respectively.When proteins were compared between the two charges, higher numbers of proteins were found for arginine-coated positively charged SiO2 nanoparticles than for the negatively charged nanoparticles.Proteins bound on the surface of nanoparticles may affect functional and conformational properties and distributions in complicated biological processes.

View Article: PubMed Central - PubMed

Affiliation: Department of Bionano Technology, Gachon Medical Research Institute, Gachon University, Sungnam-si, South Korea.

ABSTRACT
A multitude of nanoparticles, such as titanium oxide (TiO2), zinc oxide, aluminum oxide, gold oxide, silver oxide, iron oxide, and silica oxide, are found in many chemical, cosmetic, pharmaceutical, and electronic products. Recently, SiO2 nanoparticles were shown to have an inert toxicity profile and no association with an irreversible toxicological change in animal models. Hence, exposure to SiO2 nanoparticles is on the increase. SiO2 nanoparticles are routinely used in numerous materials, from strengthening filler for concrete and other construction composites, to nontoxic platforms for biomedical application, such as drug delivery and theragnostics. On the other hand, recent in vitro experiments indicated that SiO2 nanoparticles were cytotoxic. Therefore, we investigated these nanoparticles to identify potentially toxic pathways by analyzing the adsorbed protein corona on the surface of SiO2 nanoparticles in the blood and brain of the rat. Four types of SiO2 nanoparticles were chosen for investigation, and the protein corona of each type was analyzed using liquid chromatography-tandem mass spectrometry technology. In total, 115 and 48 plasma proteins from the rat were identified as being bound to negatively charged 20 nm and 100 nm SiO2 nanoparticles, respectively, and 50 and 36 proteins were found for 20 nm and 100 nm arginine-coated SiO2 nanoparticles, respectively. Higher numbers of proteins were adsorbed onto the 20 nm sized SiO2 nanoparticles than onto the 100 nm sized nanoparticles regardless of charge. When proteins were compared between the two charges, higher numbers of proteins were found for arginine-coated positively charged SiO2 nanoparticles than for the negatively charged nanoparticles. The proteins identified as bound in the corona from SiO2 nanoparticles were further analyzed with ClueGO, a Cytoscape plugin used in protein ontology and for identifying biological interaction pathways. Proteins bound on the surface of nanoparticles may affect functional and conformational properties and distributions in complicated biological processes.

No MeSH data available.


Related in: MedlinePlus