Limits...
Organization of research team for nano-associated safety assessment in effort to study nanotoxicology of zinc oxide and silica nanoparticles.

Kim YR, Park SH, Lee JK, Jeong J, Kim JH, Meang EH, Yoon TH, Lim ST, Oh JM, An SS, Kim MK - Int J Nanomedicine (2014)

Bottom Line: Currently, products made with nanomaterials are used widely, especially in biology, bio-technologies, and medical areas.Different sizes of zinc oxide and silica NPs were purchased and coated with citrate, L-serine, and L-arginine in order to modify surface charges (eight different NPs), and each of the NPs were characterized by various techniques, for example, zeta potentials, scanning electron microscopy, and transmission electron microscopy.Several limitations were encountered in the RT-NASA project, and they are discussed for consideration for improvements in future studies.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Korea University Medical School and College, Seoul, South Korea.

ABSTRACT
Currently, products made with nanomaterials are used widely, especially in biology, bio-technologies, and medical areas. However, limited investigations on potential toxicities of nanomaterials are available. Hence, diverse and systemic toxicological data with new methods for nanomaterials are needed. In order to investigate the nanotoxicology of nanoparticles (NPs), the Research Team for Nano-Associated Safety Assessment (RT-NASA) was organized in three parts and launched. Each part focused on different contents of research directions: investigators in part I were responsible for the efficient management and international cooperation on nano-safety studies; investigators in part II performed the toxicity evaluations on target organs such as assessment of genotoxicity, immunotoxicity, or skin penetration; and investigators in part III evaluated the toxicokinetics of NPs with newly developed techniques for toxicokinetic analyses and methods for estimating nanotoxicity. The RT-NASA study was carried out in six steps: need assessment, physicochemical property, toxicity evaluation, toxicokinetics, peer review, and risk communication. During the need assessment step, consumer responses were analyzed based on sex, age, education level, and household income. Different sizes of zinc oxide and silica NPs were purchased and coated with citrate, L-serine, and L-arginine in order to modify surface charges (eight different NPs), and each of the NPs were characterized by various techniques, for example, zeta potentials, scanning electron microscopy, and transmission electron microscopy. Evaluation of the "no observed adverse effect level" and systemic toxicities of all NPs were performed by thorough evaluation steps and the toxicokinetics step, which included in vivo studies with zinc oxide and silica NPs. A peer review committee was organized to evaluate and verify the reliability of toxicity tests, and the risk communication step was also needed to convey the current findings to academia, industry, and consumers. Several limitations were encountered in the RT-NASA project, and they are discussed for consideration for improvements in future studies.

No MeSH data available.


Related in: MedlinePlus

Six functional steps of the nanosafety process established by the Research Team for Nano-Associated Safety Assessment (RT-NASA).
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f2-ijn-9-003: Six functional steps of the nanosafety process established by the Research Team for Nano-Associated Safety Assessment (RT-NASA).

Mentions: Figure 2 presents the six steps for nanosafety and assessment studies of two different types of NPs, silica (SiO2) and zinc oxide (ZnO), in RT-NASA: need assessment, physicochemical property, toxicity evaluation, toxicokinetics, peer review, and risk communication. SiO2 NPs are currently being used in chemical–mechanical polishing, varnishes, printer toners, cosmetics, foodstuffs, and biomedical devices.7,8 In addition, ZnO NPs have commonly been used primarily in sunscreen products because they absorb ultraviolet light,9,10 and they have been explored as photoconductive materials in electronics, including cellular phones and iPods.11,12 During the assessment step, the latest results from various investigations of nanotoxicity and international cooperation studies were analyzed. Studies on the absorption, biodistribution, metabolism, and persistence of NPs due to their physicochemical properties and the various routes of exposure were discussed and the decision to perform subsequent assessments was determined. Toxicity evaluations of NPs on target organs by assessment of genotoxicity, immunotoxicity, dermal toxicity, reproductive and developmental toxicity of NPs by oral administration, or skin penetration were then examined. Test methods for evaluating nanotoxicity were developed and evaluated. A possible patent on this developed analytical method for in vitro characterization of NPs could be one of the outstanding outcomes of the RT-NASA project. A study focusing on risk communication between consumers and industries regarding nanosafety investigations was also included in the six steps of the RT-NASA. The interpreted results established by the RT-NASA are summarized in Table 1.


Organization of research team for nano-associated safety assessment in effort to study nanotoxicology of zinc oxide and silica nanoparticles.

Kim YR, Park SH, Lee JK, Jeong J, Kim JH, Meang EH, Yoon TH, Lim ST, Oh JM, An SS, Kim MK - Int J Nanomedicine (2014)

Six functional steps of the nanosafety process established by the Research Team for Nano-Associated Safety Assessment (RT-NASA).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4279765&req=5

f2-ijn-9-003: Six functional steps of the nanosafety process established by the Research Team for Nano-Associated Safety Assessment (RT-NASA).
Mentions: Figure 2 presents the six steps for nanosafety and assessment studies of two different types of NPs, silica (SiO2) and zinc oxide (ZnO), in RT-NASA: need assessment, physicochemical property, toxicity evaluation, toxicokinetics, peer review, and risk communication. SiO2 NPs are currently being used in chemical–mechanical polishing, varnishes, printer toners, cosmetics, foodstuffs, and biomedical devices.7,8 In addition, ZnO NPs have commonly been used primarily in sunscreen products because they absorb ultraviolet light,9,10 and they have been explored as photoconductive materials in electronics, including cellular phones and iPods.11,12 During the assessment step, the latest results from various investigations of nanotoxicity and international cooperation studies were analyzed. Studies on the absorption, biodistribution, metabolism, and persistence of NPs due to their physicochemical properties and the various routes of exposure were discussed and the decision to perform subsequent assessments was determined. Toxicity evaluations of NPs on target organs by assessment of genotoxicity, immunotoxicity, dermal toxicity, reproductive and developmental toxicity of NPs by oral administration, or skin penetration were then examined. Test methods for evaluating nanotoxicity were developed and evaluated. A possible patent on this developed analytical method for in vitro characterization of NPs could be one of the outstanding outcomes of the RT-NASA project. A study focusing on risk communication between consumers and industries regarding nanosafety investigations was also included in the six steps of the RT-NASA. The interpreted results established by the RT-NASA are summarized in Table 1.

Bottom Line: Currently, products made with nanomaterials are used widely, especially in biology, bio-technologies, and medical areas.Different sizes of zinc oxide and silica NPs were purchased and coated with citrate, L-serine, and L-arginine in order to modify surface charges (eight different NPs), and each of the NPs were characterized by various techniques, for example, zeta potentials, scanning electron microscopy, and transmission electron microscopy.Several limitations were encountered in the RT-NASA project, and they are discussed for consideration for improvements in future studies.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Korea University Medical School and College, Seoul, South Korea.

ABSTRACT
Currently, products made with nanomaterials are used widely, especially in biology, bio-technologies, and medical areas. However, limited investigations on potential toxicities of nanomaterials are available. Hence, diverse and systemic toxicological data with new methods for nanomaterials are needed. In order to investigate the nanotoxicology of nanoparticles (NPs), the Research Team for Nano-Associated Safety Assessment (RT-NASA) was organized in three parts and launched. Each part focused on different contents of research directions: investigators in part I were responsible for the efficient management and international cooperation on nano-safety studies; investigators in part II performed the toxicity evaluations on target organs such as assessment of genotoxicity, immunotoxicity, or skin penetration; and investigators in part III evaluated the toxicokinetics of NPs with newly developed techniques for toxicokinetic analyses and methods for estimating nanotoxicity. The RT-NASA study was carried out in six steps: need assessment, physicochemical property, toxicity evaluation, toxicokinetics, peer review, and risk communication. During the need assessment step, consumer responses were analyzed based on sex, age, education level, and household income. Different sizes of zinc oxide and silica NPs were purchased and coated with citrate, L-serine, and L-arginine in order to modify surface charges (eight different NPs), and each of the NPs were characterized by various techniques, for example, zeta potentials, scanning electron microscopy, and transmission electron microscopy. Evaluation of the "no observed adverse effect level" and systemic toxicities of all NPs were performed by thorough evaluation steps and the toxicokinetics step, which included in vivo studies with zinc oxide and silica NPs. A peer review committee was organized to evaluate and verify the reliability of toxicity tests, and the risk communication step was also needed to convey the current findings to academia, industry, and consumers. Several limitations were encountered in the RT-NASA project, and they are discussed for consideration for improvements in future studies.

No MeSH data available.


Related in: MedlinePlus