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Optical imaging of absorption and distribution of RITC-SiO2 nanoparticles after oral administration.

Lee CM, Lee TK, Kim DI, Kim YR, Kim MK, Jeong HJ, Sohn MH, Lim ST - Int J Nanomedicine (2014)

Bottom Line: In the ex vivo imaging studies, optical signals were observed mostly in the lungs, liver, pancreas, and kidneys.The 20 nm RITC-SiNPs showed higher uptake in the lungs than the 100 nm RITC-SiNPs.The distribution of the 100 nm RITC-SiNPs in the liver was higher than that of the 20 nm RITC-SiNPs, but the differences in the surface charge behavior were imperceptible.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Chonnam National University, Yeosu, Jeollanam-Do, Republic of Korea.

ABSTRACT

Purpose: In this study, we investigated the absorption and distribution of rhodamine B isothiocyanate (RITC)-incorporated silica oxide nanoparticles(SiNPs) (RITC-SiNPs) after oral exposure, by conducting optical imaging, with a focus on tracking the movement of RITC-SiNPs of different particle size and surface charge.

Methods: RITC-SiNPs (20 or 100 nm; positively or negatively charged) were used to avoid the dissociation of a fluorescent dye from nanoparticles via spontaneous or enzyme-catalyzed reactions in vivo. The changes in the nanoparticle sizes and shapes were investigated in an HCl solution for 6 hours. RITC-SiNPs were orally administered to healthy nude mice at a dose of 100 mg/kg. Optical imaging studies were performed at 2, 4, and 6 hours after oral administration. The mice were sacrificed at 2, 4, 6, and 10 hours post-administration, and ex vivo imaging studies were performed.

Results: The RITC-SiNPs were stable in the HCl solution for 6 hours, without dissociation of RITC from the nanoparticles and without changes in size and shape. RITC-SiNPs flowed into the small intestine from the stomach and gradually moved along the gut during the experiment. In the ex vivo imaging studies, optical signals were observed mostly in the lungs, liver, pancreas, and kidneys. The orally administered RITC-SiNPs, which were absorbed in the systemic circulation, were eliminated from the body into the urine. The 20 nm RITC-SiNPs showed higher uptake in the lungs than the 100 nm RITC-SiNPs. The distribution of the 100 nm RITC-SiNPs in the liver was higher than that of the 20 nm RITC-SiNPs, but the differences in the surface charge behavior were imperceptible.

Conclusion: We demonstrated that the movement of RITC-SiNPs after oral exposure could be traced by optical imaging. Optical imaging has the potential to provide valuable information that will help in understanding the behavior of SiNPs in the body following exposure.

No MeSH data available.


Related in: MedlinePlus

Ex vivo optical images of the major organs in mice that were orally administered with (A) RITC-SiNPs20(+), (B) RITC-SiNPs20(−), (C) RITC-SiNPs100(+), and (D) RITC-SiNPs100(−) at 2, 4, 6, and 10 hours post-administration. (E) The organs were removed from a healthy mouse without administration of RITC-SiNPs and imaged under the same conditions.Abbreviations: Bon, bone; Br, brain; Ht, heart; Kid, kidneys; Li, liver; Lu, lungs; Mus, muscle; Pan, pancreas; RITC-SiNP, rhodamine B isothiocyanate-incorporated silica oxide nanoparticle; RITC-SiNPs20(+), 20 nm positively charged RITC-SiNPs; RITC-SiNPs20(−), 20 nm negatively charged RITC-SiNPs; RITC-SiNPs100(+), 100 nm positively charged RITC-SiNPs; RITC-SiNPs100(−), 100 nm negatively charged RITC-SiNPs; Sp, spleen; h, hours.
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f6-ijn-9-243: Ex vivo optical images of the major organs in mice that were orally administered with (A) RITC-SiNPs20(+), (B) RITC-SiNPs20(−), (C) RITC-SiNPs100(+), and (D) RITC-SiNPs100(−) at 2, 4, 6, and 10 hours post-administration. (E) The organs were removed from a healthy mouse without administration of RITC-SiNPs and imaged under the same conditions.Abbreviations: Bon, bone; Br, brain; Ht, heart; Kid, kidneys; Li, liver; Lu, lungs; Mus, muscle; Pan, pancreas; RITC-SiNP, rhodamine B isothiocyanate-incorporated silica oxide nanoparticle; RITC-SiNPs20(+), 20 nm positively charged RITC-SiNPs; RITC-SiNPs20(−), 20 nm negatively charged RITC-SiNPs; RITC-SiNPs100(+), 100 nm positively charged RITC-SiNPs; RITC-SiNPs100(−), 100 nm negatively charged RITC-SiNPs; Sp, spleen; h, hours.

Mentions: Figure 5 shows the whole-body optical fluorescence images of mice after oral administration of the RITC-SiNPs. The fluorescence signals from the RITC-SiNPs were mainly visualized in the GI tract of the mice after oral administration of the RITC-SiNPs. Ex vivo optical images of the major organs from the mice are presented in Figure 6. Quantification results of ex vivo images were shown in Figure 7. After their oral administration of the RITC-SiNPs, majority of them was found to be distributed in the lungs, liver, pancreas, and kidneys. The RITC-SiNPs with a diameter of 20 nm showed higher uptake in the lungs than the RITC-SiNPs with a diameter of 100 nm. After their oral administration, no differences in the translocation and distribution of RITC-SiNPs were observed because of their surface charges. At 10 hours post-administration, the majority of 20 nm RITC-SiNPs were cleared from the organs, and they were visualized in the kidneys. On the other hand, the 100 nm RITC-SiNPs were distributed mainly in the liver and kidneys, and they were cleared from the organs except for the kidneys at 10 hours after oral administration.


Optical imaging of absorption and distribution of RITC-SiO2 nanoparticles after oral administration.

Lee CM, Lee TK, Kim DI, Kim YR, Kim MK, Jeong HJ, Sohn MH, Lim ST - Int J Nanomedicine (2014)

Ex vivo optical images of the major organs in mice that were orally administered with (A) RITC-SiNPs20(+), (B) RITC-SiNPs20(−), (C) RITC-SiNPs100(+), and (D) RITC-SiNPs100(−) at 2, 4, 6, and 10 hours post-administration. (E) The organs were removed from a healthy mouse without administration of RITC-SiNPs and imaged under the same conditions.Abbreviations: Bon, bone; Br, brain; Ht, heart; Kid, kidneys; Li, liver; Lu, lungs; Mus, muscle; Pan, pancreas; RITC-SiNP, rhodamine B isothiocyanate-incorporated silica oxide nanoparticle; RITC-SiNPs20(+), 20 nm positively charged RITC-SiNPs; RITC-SiNPs20(−), 20 nm negatively charged RITC-SiNPs; RITC-SiNPs100(+), 100 nm positively charged RITC-SiNPs; RITC-SiNPs100(−), 100 nm negatively charged RITC-SiNPs; Sp, spleen; h, hours.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4279756&req=5

f6-ijn-9-243: Ex vivo optical images of the major organs in mice that were orally administered with (A) RITC-SiNPs20(+), (B) RITC-SiNPs20(−), (C) RITC-SiNPs100(+), and (D) RITC-SiNPs100(−) at 2, 4, 6, and 10 hours post-administration. (E) The organs were removed from a healthy mouse without administration of RITC-SiNPs and imaged under the same conditions.Abbreviations: Bon, bone; Br, brain; Ht, heart; Kid, kidneys; Li, liver; Lu, lungs; Mus, muscle; Pan, pancreas; RITC-SiNP, rhodamine B isothiocyanate-incorporated silica oxide nanoparticle; RITC-SiNPs20(+), 20 nm positively charged RITC-SiNPs; RITC-SiNPs20(−), 20 nm negatively charged RITC-SiNPs; RITC-SiNPs100(+), 100 nm positively charged RITC-SiNPs; RITC-SiNPs100(−), 100 nm negatively charged RITC-SiNPs; Sp, spleen; h, hours.
Mentions: Figure 5 shows the whole-body optical fluorescence images of mice after oral administration of the RITC-SiNPs. The fluorescence signals from the RITC-SiNPs were mainly visualized in the GI tract of the mice after oral administration of the RITC-SiNPs. Ex vivo optical images of the major organs from the mice are presented in Figure 6. Quantification results of ex vivo images were shown in Figure 7. After their oral administration of the RITC-SiNPs, majority of them was found to be distributed in the lungs, liver, pancreas, and kidneys. The RITC-SiNPs with a diameter of 20 nm showed higher uptake in the lungs than the RITC-SiNPs with a diameter of 100 nm. After their oral administration, no differences in the translocation and distribution of RITC-SiNPs were observed because of their surface charges. At 10 hours post-administration, the majority of 20 nm RITC-SiNPs were cleared from the organs, and they were visualized in the kidneys. On the other hand, the 100 nm RITC-SiNPs were distributed mainly in the liver and kidneys, and they were cleared from the organs except for the kidneys at 10 hours after oral administration.

Bottom Line: In the ex vivo imaging studies, optical signals were observed mostly in the lungs, liver, pancreas, and kidneys.The 20 nm RITC-SiNPs showed higher uptake in the lungs than the 100 nm RITC-SiNPs.The distribution of the 100 nm RITC-SiNPs in the liver was higher than that of the 20 nm RITC-SiNPs, but the differences in the surface charge behavior were imperceptible.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Chonnam National University, Yeosu, Jeollanam-Do, Republic of Korea.

ABSTRACT

Purpose: In this study, we investigated the absorption and distribution of rhodamine B isothiocyanate (RITC)-incorporated silica oxide nanoparticles(SiNPs) (RITC-SiNPs) after oral exposure, by conducting optical imaging, with a focus on tracking the movement of RITC-SiNPs of different particle size and surface charge.

Methods: RITC-SiNPs (20 or 100 nm; positively or negatively charged) were used to avoid the dissociation of a fluorescent dye from nanoparticles via spontaneous or enzyme-catalyzed reactions in vivo. The changes in the nanoparticle sizes and shapes were investigated in an HCl solution for 6 hours. RITC-SiNPs were orally administered to healthy nude mice at a dose of 100 mg/kg. Optical imaging studies were performed at 2, 4, and 6 hours after oral administration. The mice were sacrificed at 2, 4, 6, and 10 hours post-administration, and ex vivo imaging studies were performed.

Results: The RITC-SiNPs were stable in the HCl solution for 6 hours, without dissociation of RITC from the nanoparticles and without changes in size and shape. RITC-SiNPs flowed into the small intestine from the stomach and gradually moved along the gut during the experiment. In the ex vivo imaging studies, optical signals were observed mostly in the lungs, liver, pancreas, and kidneys. The orally administered RITC-SiNPs, which were absorbed in the systemic circulation, were eliminated from the body into the urine. The 20 nm RITC-SiNPs showed higher uptake in the lungs than the 100 nm RITC-SiNPs. The distribution of the 100 nm RITC-SiNPs in the liver was higher than that of the 20 nm RITC-SiNPs, but the differences in the surface charge behavior were imperceptible.

Conclusion: We demonstrated that the movement of RITC-SiNPs after oral exposure could be traced by optical imaging. Optical imaging has the potential to provide valuable information that will help in understanding the behavior of SiNPs in the body following exposure.

No MeSH data available.


Related in: MedlinePlus