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Effect of zinc oxide nanoparticles on dams and embryo-fetal development in rats.

Hong JS, Park MK, Kim MS, Lim JH, Park GJ, Maeng EH, Shin JH, Kim YR, Kim MK, Lee JK, Park JA, Kim JC, Shin HC - Int J Nanomedicine (2014)

Bottom Line: Toxicity in the dams manifested as significantly decreased body weight at 400 mg/kg/day and decreased liver weight, and increased adrenal glands weight at 200 mg/kg/day and 400 mg/kg/day.No significant difference was found in the Zn content of fetal tissue between the control and high-dose groups.These results showed that a 15-day repeated oral dose of ZnO(SM20(-)) was minimally maternotoxic at dose of 200 mg/kg/day and 400 mg/kg/day.

View Article: PubMed Central - PubMed

Affiliation: Health Care Research Laboratory, Korea Testing and Research Institute, Gimpo, Korea ; College of Veterinary Medicine, Konkuk University, Seoul, Korea.

ABSTRACT
This study investigated the potential adverse effects of zinc oxide nanoparticles (ZnO(SM20[-]) NPs; negatively charged, 20 nm) on pregnant dams and embryo-fetal development after maternal exposure over the period of gestational days 5-19 with Sprague Dawley rats. ZnO(SM20(-)) NPs were administered to pregnant rats by gavage at 0 mg/kg/day, 100 mg/kg/day, 200 mg/kg/day, and 400 mg/kg/day. All dams were subjected to caesarean section on gestational day 20, and all the fetuses were examined for external, visceral, and skeletal alterations. Toxicity in the dams manifested as significantly decreased body weight at 400 mg/kg/day and decreased liver weight, and increased adrenal glands weight at 200 mg/kg/day and 400 mg/kg/day. However, no treatment-related difference in the number of corpora lutea, the number of implantation sites, the implantation rate (%), resorption, dead fetuses, litter size, fetal deaths, fetal and placental weights, and sex ratio were observed between the groups. Morphological examinations of the fetuses demonstrated no significant difference in the incidences of abnormalities between the groups. No significant difference was found in the Zn content of fetal tissue between the control and high-dose groups. These results showed that a 15-day repeated oral dose of ZnO(SM20(-)) was minimally maternotoxic at dose of 200 mg/kg/day and 400 mg/kg/day.

No MeSH data available.


Related in: MedlinePlus

Body weight changes of female rats during the gestation period.Notes: Pregnant rats were orally treated with ZnOSM20(−) NPs for 15 days (GD 5–GD 19) with doses of 100 mg/kg/day, 200 mg/kg/day, and 400 mg/kg/day. GD 0 means the day of pregnancy. “GD” is the day after gestation. Statistically different from the vehicle control group; *P<0.05.Abbreviations: GD, gestational day; ZnOSM20(−), 20 nm negatively-charged ZnO; NPs, nanoparticles.
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f2-ijn-9-145: Body weight changes of female rats during the gestation period.Notes: Pregnant rats were orally treated with ZnOSM20(−) NPs for 15 days (GD 5–GD 19) with doses of 100 mg/kg/day, 200 mg/kg/day, and 400 mg/kg/day. GD 0 means the day of pregnancy. “GD” is the day after gestation. Statistically different from the vehicle control group; *P<0.05.Abbreviations: GD, gestational day; ZnOSM20(−), 20 nm negatively-charged ZnO; NPs, nanoparticles.

Mentions: Six of 21 dams from the 100 mg/kg/day group, ten of 23 dams from the 200 mg/kg/day group, and 16 of 25 dams from the 400 mg/kg/day group showed salivation around mouth in terms of their general appearance, but it was transiently observed after treatment. Also, alopecia (localized areas of partial alopecia) was observed in two pregnant rats from the vehicle control group, one from the 100 mg/kg/day group, one from the 200 mg/kg/day group, and two from the 400 mg/kg/day group, starting from 6–10 days after oral administration (data not shown). This clinical sign was not recovered during the treatment period. The changes in body weight during the entire experimental period are shown in Figure 2. As shown in the data of Table 3, the maternal body weight on GD 20 in the high-dose group was significantly decreased when compared with the vehicle control group. The maternal body weight gain during pregnancy (P<0.05) and corrected body weight (P<0.01) were also significantly lower than those for the control group. There was no statistically significant difference in food consumption between the control and treatment groups (Table 4). At the scheduled autopsy, no treatment-related gross finding was observed in the dams from all groups. The absolute and relative organ weights of the pregnant rats, treated with ZnOSM20(−) NPs, are presented in Table 5. The relative liver weight in the 200 mg/kg/day group, and the absolute and relative liver weights in the 400 mg/kg/day group were significantly decreased in a dose-dependent manner in comparison with those of the vehicle control group. Absolute and relative weights of the right adrenal gland in the 200 mg/kg/day and 400 mg/kg/day groups and relative weights of the left adrenal gland in the 400 mg/kg/day group were significantly increased in a dose-dependent manner in comparison with the vehicle control group.


Effect of zinc oxide nanoparticles on dams and embryo-fetal development in rats.

Hong JS, Park MK, Kim MS, Lim JH, Park GJ, Maeng EH, Shin JH, Kim YR, Kim MK, Lee JK, Park JA, Kim JC, Shin HC - Int J Nanomedicine (2014)

Body weight changes of female rats during the gestation period.Notes: Pregnant rats were orally treated with ZnOSM20(−) NPs for 15 days (GD 5–GD 19) with doses of 100 mg/kg/day, 200 mg/kg/day, and 400 mg/kg/day. GD 0 means the day of pregnancy. “GD” is the day after gestation. Statistically different from the vehicle control group; *P<0.05.Abbreviations: GD, gestational day; ZnOSM20(−), 20 nm negatively-charged ZnO; NPs, nanoparticles.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4279755&req=5

f2-ijn-9-145: Body weight changes of female rats during the gestation period.Notes: Pregnant rats were orally treated with ZnOSM20(−) NPs for 15 days (GD 5–GD 19) with doses of 100 mg/kg/day, 200 mg/kg/day, and 400 mg/kg/day. GD 0 means the day of pregnancy. “GD” is the day after gestation. Statistically different from the vehicle control group; *P<0.05.Abbreviations: GD, gestational day; ZnOSM20(−), 20 nm negatively-charged ZnO; NPs, nanoparticles.
Mentions: Six of 21 dams from the 100 mg/kg/day group, ten of 23 dams from the 200 mg/kg/day group, and 16 of 25 dams from the 400 mg/kg/day group showed salivation around mouth in terms of their general appearance, but it was transiently observed after treatment. Also, alopecia (localized areas of partial alopecia) was observed in two pregnant rats from the vehicle control group, one from the 100 mg/kg/day group, one from the 200 mg/kg/day group, and two from the 400 mg/kg/day group, starting from 6–10 days after oral administration (data not shown). This clinical sign was not recovered during the treatment period. The changes in body weight during the entire experimental period are shown in Figure 2. As shown in the data of Table 3, the maternal body weight on GD 20 in the high-dose group was significantly decreased when compared with the vehicle control group. The maternal body weight gain during pregnancy (P<0.05) and corrected body weight (P<0.01) were also significantly lower than those for the control group. There was no statistically significant difference in food consumption between the control and treatment groups (Table 4). At the scheduled autopsy, no treatment-related gross finding was observed in the dams from all groups. The absolute and relative organ weights of the pregnant rats, treated with ZnOSM20(−) NPs, are presented in Table 5. The relative liver weight in the 200 mg/kg/day group, and the absolute and relative liver weights in the 400 mg/kg/day group were significantly decreased in a dose-dependent manner in comparison with those of the vehicle control group. Absolute and relative weights of the right adrenal gland in the 200 mg/kg/day and 400 mg/kg/day groups and relative weights of the left adrenal gland in the 400 mg/kg/day group were significantly increased in a dose-dependent manner in comparison with the vehicle control group.

Bottom Line: Toxicity in the dams manifested as significantly decreased body weight at 400 mg/kg/day and decreased liver weight, and increased adrenal glands weight at 200 mg/kg/day and 400 mg/kg/day.No significant difference was found in the Zn content of fetal tissue between the control and high-dose groups.These results showed that a 15-day repeated oral dose of ZnO(SM20(-)) was minimally maternotoxic at dose of 200 mg/kg/day and 400 mg/kg/day.

View Article: PubMed Central - PubMed

Affiliation: Health Care Research Laboratory, Korea Testing and Research Institute, Gimpo, Korea ; College of Veterinary Medicine, Konkuk University, Seoul, Korea.

ABSTRACT
This study investigated the potential adverse effects of zinc oxide nanoparticles (ZnO(SM20[-]) NPs; negatively charged, 20 nm) on pregnant dams and embryo-fetal development after maternal exposure over the period of gestational days 5-19 with Sprague Dawley rats. ZnO(SM20(-)) NPs were administered to pregnant rats by gavage at 0 mg/kg/day, 100 mg/kg/day, 200 mg/kg/day, and 400 mg/kg/day. All dams were subjected to caesarean section on gestational day 20, and all the fetuses were examined for external, visceral, and skeletal alterations. Toxicity in the dams manifested as significantly decreased body weight at 400 mg/kg/day and decreased liver weight, and increased adrenal glands weight at 200 mg/kg/day and 400 mg/kg/day. However, no treatment-related difference in the number of corpora lutea, the number of implantation sites, the implantation rate (%), resorption, dead fetuses, litter size, fetal deaths, fetal and placental weights, and sex ratio were observed between the groups. Morphological examinations of the fetuses demonstrated no significant difference in the incidences of abnormalities between the groups. No significant difference was found in the Zn content of fetal tissue between the control and high-dose groups. These results showed that a 15-day repeated oral dose of ZnO(SM20(-)) was minimally maternotoxic at dose of 200 mg/kg/day and 400 mg/kg/day.

No MeSH data available.


Related in: MedlinePlus