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Effect of zinc oxide nanoparticles on dams and embryo-fetal development in rats.

Hong JS, Park MK, Kim MS, Lim JH, Park GJ, Maeng EH, Shin JH, Kim YR, Kim MK, Lee JK, Park JA, Kim JC, Shin HC - Int J Nanomedicine (2014)

Bottom Line: Toxicity in the dams manifested as significantly decreased body weight at 400 mg/kg/day and decreased liver weight, and increased adrenal glands weight at 200 mg/kg/day and 400 mg/kg/day.No significant difference was found in the Zn content of fetal tissue between the control and high-dose groups.These results showed that a 15-day repeated oral dose of ZnO(SM20(-)) was minimally maternotoxic at dose of 200 mg/kg/day and 400 mg/kg/day.

View Article: PubMed Central - PubMed

Affiliation: Health Care Research Laboratory, Korea Testing and Research Institute, Gimpo, Korea ; College of Veterinary Medicine, Konkuk University, Seoul, Korea.

ABSTRACT
This study investigated the potential adverse effects of zinc oxide nanoparticles (ZnO(SM20[-]) NPs; negatively charged, 20 nm) on pregnant dams and embryo-fetal development after maternal exposure over the period of gestational days 5-19 with Sprague Dawley rats. ZnO(SM20(-)) NPs were administered to pregnant rats by gavage at 0 mg/kg/day, 100 mg/kg/day, 200 mg/kg/day, and 400 mg/kg/day. All dams were subjected to caesarean section on gestational day 20, and all the fetuses were examined for external, visceral, and skeletal alterations. Toxicity in the dams manifested as significantly decreased body weight at 400 mg/kg/day and decreased liver weight, and increased adrenal glands weight at 200 mg/kg/day and 400 mg/kg/day. However, no treatment-related difference in the number of corpora lutea, the number of implantation sites, the implantation rate (%), resorption, dead fetuses, litter size, fetal deaths, fetal and placental weights, and sex ratio were observed between the groups. Morphological examinations of the fetuses demonstrated no significant difference in the incidences of abnormalities between the groups. No significant difference was found in the Zn content of fetal tissue between the control and high-dose groups. These results showed that a 15-day repeated oral dose of ZnO(SM20(-)) was minimally maternotoxic at dose of 200 mg/kg/day and 400 mg/kg/day.

No MeSH data available.


Related in: MedlinePlus

The total Zn levels measured with ICP-AES.Notes: To investigate the placenta transfer of ZnOSM20(−) NPs in vivo, four extra female rats were used in the nontreatment control group (n=2) and 400 mg/kg/day groups (ZnOSM20[−] NPs; n=2), respectively. Dosing occurred on GDs 5–19 in the same manner as for the main study animals.Abbreviations: ZnOSM20(−), 20 nm negatively-charged ZnO; NPs, nanoparticles; ICP-AES, inductive coupled plasma atomic emission spectrometry; n, number; GD, gestational day.
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f1-ijn-9-145: The total Zn levels measured with ICP-AES.Notes: To investigate the placenta transfer of ZnOSM20(−) NPs in vivo, four extra female rats were used in the nontreatment control group (n=2) and 400 mg/kg/day groups (ZnOSM20[−] NPs; n=2), respectively. Dosing occurred on GDs 5–19 in the same manner as for the main study animals.Abbreviations: ZnOSM20(−), 20 nm negatively-charged ZnO; NPs, nanoparticles; ICP-AES, inductive coupled plasma atomic emission spectrometry; n, number; GD, gestational day.

Mentions: The Zn concentrations in fetal tissues are shown in Figure 1. The measured total Zn levels with ICP-AES was 14.44±0.37 μg/g (mean ± standard deviation) for the control group, and 19.02±0.60 μg/g (ZnOSM20[−] NPs) for the 400 mg/kg/day group (Table 2). The Zn contents in fetuses after in utero exposure to ZnOSM20(−) NPs were not significantly different from the Zn contents in control fetuses.


Effect of zinc oxide nanoparticles on dams and embryo-fetal development in rats.

Hong JS, Park MK, Kim MS, Lim JH, Park GJ, Maeng EH, Shin JH, Kim YR, Kim MK, Lee JK, Park JA, Kim JC, Shin HC - Int J Nanomedicine (2014)

The total Zn levels measured with ICP-AES.Notes: To investigate the placenta transfer of ZnOSM20(−) NPs in vivo, four extra female rats were used in the nontreatment control group (n=2) and 400 mg/kg/day groups (ZnOSM20[−] NPs; n=2), respectively. Dosing occurred on GDs 5–19 in the same manner as for the main study animals.Abbreviations: ZnOSM20(−), 20 nm negatively-charged ZnO; NPs, nanoparticles; ICP-AES, inductive coupled plasma atomic emission spectrometry; n, number; GD, gestational day.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4279755&req=5

f1-ijn-9-145: The total Zn levels measured with ICP-AES.Notes: To investigate the placenta transfer of ZnOSM20(−) NPs in vivo, four extra female rats were used in the nontreatment control group (n=2) and 400 mg/kg/day groups (ZnOSM20[−] NPs; n=2), respectively. Dosing occurred on GDs 5–19 in the same manner as for the main study animals.Abbreviations: ZnOSM20(−), 20 nm negatively-charged ZnO; NPs, nanoparticles; ICP-AES, inductive coupled plasma atomic emission spectrometry; n, number; GD, gestational day.
Mentions: The Zn concentrations in fetal tissues are shown in Figure 1. The measured total Zn levels with ICP-AES was 14.44±0.37 μg/g (mean ± standard deviation) for the control group, and 19.02±0.60 μg/g (ZnOSM20[−] NPs) for the 400 mg/kg/day group (Table 2). The Zn contents in fetuses after in utero exposure to ZnOSM20(−) NPs were not significantly different from the Zn contents in control fetuses.

Bottom Line: Toxicity in the dams manifested as significantly decreased body weight at 400 mg/kg/day and decreased liver weight, and increased adrenal glands weight at 200 mg/kg/day and 400 mg/kg/day.No significant difference was found in the Zn content of fetal tissue between the control and high-dose groups.These results showed that a 15-day repeated oral dose of ZnO(SM20(-)) was minimally maternotoxic at dose of 200 mg/kg/day and 400 mg/kg/day.

View Article: PubMed Central - PubMed

Affiliation: Health Care Research Laboratory, Korea Testing and Research Institute, Gimpo, Korea ; College of Veterinary Medicine, Konkuk University, Seoul, Korea.

ABSTRACT
This study investigated the potential adverse effects of zinc oxide nanoparticles (ZnO(SM20[-]) NPs; negatively charged, 20 nm) on pregnant dams and embryo-fetal development after maternal exposure over the period of gestational days 5-19 with Sprague Dawley rats. ZnO(SM20(-)) NPs were administered to pregnant rats by gavage at 0 mg/kg/day, 100 mg/kg/day, 200 mg/kg/day, and 400 mg/kg/day. All dams were subjected to caesarean section on gestational day 20, and all the fetuses were examined for external, visceral, and skeletal alterations. Toxicity in the dams manifested as significantly decreased body weight at 400 mg/kg/day and decreased liver weight, and increased adrenal glands weight at 200 mg/kg/day and 400 mg/kg/day. However, no treatment-related difference in the number of corpora lutea, the number of implantation sites, the implantation rate (%), resorption, dead fetuses, litter size, fetal deaths, fetal and placental weights, and sex ratio were observed between the groups. Morphological examinations of the fetuses demonstrated no significant difference in the incidences of abnormalities between the groups. No significant difference was found in the Zn content of fetal tissue between the control and high-dose groups. These results showed that a 15-day repeated oral dose of ZnO(SM20(-)) was minimally maternotoxic at dose of 200 mg/kg/day and 400 mg/kg/day.

No MeSH data available.


Related in: MedlinePlus