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Identification of new metabolites of bacterial transformation of indole by gas chromatography-mass spectrometry and high performance liquid chromatography.

Arora PK, Bae H - Int J Anal Chem (2014)

Bottom Line: Arthrobacter sp.SPG transformed indole completely in the presence of an additional carbon source.High performance liquid chromatography and gas chromatography-mass spectrometry detected indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde as biotransformation products.

View Article: PubMed Central - PubMed

Affiliation: School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Republic of Korea.

ABSTRACT
Arthrobacter sp. SPG transformed indole completely in the presence of an additional carbon source. High performance liquid chromatography and gas chromatography-mass spectrometry detected indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde as biotransformation products. This is the first report of the formation of indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde from indole by any bacterium.

No MeSH data available.


Related in: MedlinePlus

Proposed pathway of indole transformation for Arthrobacter sp. SPG.
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Related In: Results  -  Collection


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fig4: Proposed pathway of indole transformation for Arthrobacter sp. SPG.

Mentions: Indole-3-aldehyde and indole-3-glyoxylic acid have been identified as the products of the enzymatic oxidation of indole-3-acetic acid [20]. Stutz [20] also reported the enzymatic formation of indole-3-aldehyde from indole-3-acetic acid via indole-3-glyoxylic acid. We have also detected indole-3-aldehyde and indole-3-glyoxylic acid, which could be formed from indole-3-acetic acid in the indole biotransformation pathway. On the basis of the above discussion, we propose a tryptophan-independent pathway for indole biotransformation. Indole was first converted to indole-3-acetic acid via a tryptophan-independent pathway, which was further oxidized to indole-3-glyoxylic acid and then to indole-3-aldehyde (Figure 4).


Identification of new metabolites of bacterial transformation of indole by gas chromatography-mass spectrometry and high performance liquid chromatography.

Arora PK, Bae H - Int J Anal Chem (2014)

Proposed pathway of indole transformation for Arthrobacter sp. SPG.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4274814&req=5

fig4: Proposed pathway of indole transformation for Arthrobacter sp. SPG.
Mentions: Indole-3-aldehyde and indole-3-glyoxylic acid have been identified as the products of the enzymatic oxidation of indole-3-acetic acid [20]. Stutz [20] also reported the enzymatic formation of indole-3-aldehyde from indole-3-acetic acid via indole-3-glyoxylic acid. We have also detected indole-3-aldehyde and indole-3-glyoxylic acid, which could be formed from indole-3-acetic acid in the indole biotransformation pathway. On the basis of the above discussion, we propose a tryptophan-independent pathway for indole biotransformation. Indole was first converted to indole-3-acetic acid via a tryptophan-independent pathway, which was further oxidized to indole-3-glyoxylic acid and then to indole-3-aldehyde (Figure 4).

Bottom Line: Arthrobacter sp.SPG transformed indole completely in the presence of an additional carbon source.High performance liquid chromatography and gas chromatography-mass spectrometry detected indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde as biotransformation products.

View Article: PubMed Central - PubMed

Affiliation: School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Republic of Korea.

ABSTRACT
Arthrobacter sp. SPG transformed indole completely in the presence of an additional carbon source. High performance liquid chromatography and gas chromatography-mass spectrometry detected indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde as biotransformation products. This is the first report of the formation of indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde from indole by any bacterium.

No MeSH data available.


Related in: MedlinePlus