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Identification of new metabolites of bacterial transformation of indole by gas chromatography-mass spectrometry and high performance liquid chromatography.

Arora PK, Bae H - Int J Anal Chem (2014)

Bottom Line: Arthrobacter sp.SPG transformed indole completely in the presence of an additional carbon source.High performance liquid chromatography and gas chromatography-mass spectrometry detected indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde as biotransformation products.

View Article: PubMed Central - PubMed

Affiliation: School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Republic of Korea.

ABSTRACT
Arthrobacter sp. SPG transformed indole completely in the presence of an additional carbon source. High performance liquid chromatography and gas chromatography-mass spectrometry detected indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde as biotransformation products. This is the first report of the formation of indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde from indole by any bacterium.

No MeSH data available.


Related in: MedlinePlus

HPLC analysis of samples of indole biotransformation by Arthrobacter sp. SPG. (a) 8 h sample showing only peak for indole and (b) 36 h sample showing complete bacterial transformation of indole into indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde.
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Related In: Results  -  Collection


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fig2: HPLC analysis of samples of indole biotransformation by Arthrobacter sp. SPG. (a) 8 h sample showing only peak for indole and (b) 36 h sample showing complete bacterial transformation of indole into indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde.

Mentions: HPLC analysis also showed the complete transformation of indole into three metabolites (Figure 2). In the sample of 8 h, only peak of indole was detected, whereas in the sample of 36 h, indole was completely depleted with appearance of three metabolites. These metabolites were identified as indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde on the basis of the comparison of their retention times with those of authentic standards. The retention times of these three metabolites were 9.60, 12.0, and 7.48 min, respectively, whereas retention time of the indole was 4.25 min.


Identification of new metabolites of bacterial transformation of indole by gas chromatography-mass spectrometry and high performance liquid chromatography.

Arora PK, Bae H - Int J Anal Chem (2014)

HPLC analysis of samples of indole biotransformation by Arthrobacter sp. SPG. (a) 8 h sample showing only peak for indole and (b) 36 h sample showing complete bacterial transformation of indole into indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4274814&req=5

fig2: HPLC analysis of samples of indole biotransformation by Arthrobacter sp. SPG. (a) 8 h sample showing only peak for indole and (b) 36 h sample showing complete bacterial transformation of indole into indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde.
Mentions: HPLC analysis also showed the complete transformation of indole into three metabolites (Figure 2). In the sample of 8 h, only peak of indole was detected, whereas in the sample of 36 h, indole was completely depleted with appearance of three metabolites. These metabolites were identified as indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde on the basis of the comparison of their retention times with those of authentic standards. The retention times of these three metabolites were 9.60, 12.0, and 7.48 min, respectively, whereas retention time of the indole was 4.25 min.

Bottom Line: Arthrobacter sp.SPG transformed indole completely in the presence of an additional carbon source.High performance liquid chromatography and gas chromatography-mass spectrometry detected indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde as biotransformation products.

View Article: PubMed Central - PubMed

Affiliation: School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Republic of Korea.

ABSTRACT
Arthrobacter sp. SPG transformed indole completely in the presence of an additional carbon source. High performance liquid chromatography and gas chromatography-mass spectrometry detected indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde as biotransformation products. This is the first report of the formation of indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde from indole by any bacterium.

No MeSH data available.


Related in: MedlinePlus