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Identification of new metabolites of bacterial transformation of indole by gas chromatography-mass spectrometry and high performance liquid chromatography.

Arora PK, Bae H - Int J Anal Chem (2014)

Bottom Line: Arthrobacter sp.SPG transformed indole completely in the presence of an additional carbon source.High performance liquid chromatography and gas chromatography-mass spectrometry detected indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde as biotransformation products.

View Article: PubMed Central - PubMed

Affiliation: School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Republic of Korea.

ABSTRACT
Arthrobacter sp. SPG transformed indole completely in the presence of an additional carbon source. High performance liquid chromatography and gas chromatography-mass spectrometry detected indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde as biotransformation products. This is the first report of the formation of indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde from indole by any bacterium.

No MeSH data available.


Related in: MedlinePlus

Growth of Arthrobacter sp. SPG in (a) minimal medium containing 10 mM sodium succinate, (b) minimal medium containing 0.5 mM indole, and (c) minimal medium containing both 10 mM sodium succinate and 0.5 mM indole. (d) Indole depletion by Arthrobacter sp. SPG.
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Related In: Results  -  Collection


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fig1: Growth of Arthrobacter sp. SPG in (a) minimal medium containing 10 mM sodium succinate, (b) minimal medium containing 0.5 mM indole, and (c) minimal medium containing both 10 mM sodium succinate and 0.5 mM indole. (d) Indole depletion by Arthrobacter sp. SPG.

Mentions: Arthrobacter sp. SPG was able to transform 0.5 mM indole in the presence of sodium succinate. The growth of strain SPG was measured in minimal medium containing 10 mM sodium succinate and 0.5 mM indole. Strain SPG grew well in minimal medium containing 10 mM sodium succinate as its sole source of carbon and energy. However, there was no bacterial growth on minimal medium containing 0.5 mM indole as its sole source of carbon and energy. The bacterial growth was significantly reduced in medium containing 10 mM sodium succinate and 0.5 mM indole and the maximum optical density of the culture was 0.35 after 40 hours of incubation (Figure 1). Indole depletion was measured by HPLC and it was observed that indole was completely depleted/transformed within 36 h.


Identification of new metabolites of bacterial transformation of indole by gas chromatography-mass spectrometry and high performance liquid chromatography.

Arora PK, Bae H - Int J Anal Chem (2014)

Growth of Arthrobacter sp. SPG in (a) minimal medium containing 10 mM sodium succinate, (b) minimal medium containing 0.5 mM indole, and (c) minimal medium containing both 10 mM sodium succinate and 0.5 mM indole. (d) Indole depletion by Arthrobacter sp. SPG.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4274814&req=5

fig1: Growth of Arthrobacter sp. SPG in (a) minimal medium containing 10 mM sodium succinate, (b) minimal medium containing 0.5 mM indole, and (c) minimal medium containing both 10 mM sodium succinate and 0.5 mM indole. (d) Indole depletion by Arthrobacter sp. SPG.
Mentions: Arthrobacter sp. SPG was able to transform 0.5 mM indole in the presence of sodium succinate. The growth of strain SPG was measured in minimal medium containing 10 mM sodium succinate and 0.5 mM indole. Strain SPG grew well in minimal medium containing 10 mM sodium succinate as its sole source of carbon and energy. However, there was no bacterial growth on minimal medium containing 0.5 mM indole as its sole source of carbon and energy. The bacterial growth was significantly reduced in medium containing 10 mM sodium succinate and 0.5 mM indole and the maximum optical density of the culture was 0.35 after 40 hours of incubation (Figure 1). Indole depletion was measured by HPLC and it was observed that indole was completely depleted/transformed within 36 h.

Bottom Line: Arthrobacter sp.SPG transformed indole completely in the presence of an additional carbon source.High performance liquid chromatography and gas chromatography-mass spectrometry detected indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde as biotransformation products.

View Article: PubMed Central - PubMed

Affiliation: School of Biotechnology, Yeungnam University, Gyeongsan 712-749, Republic of Korea.

ABSTRACT
Arthrobacter sp. SPG transformed indole completely in the presence of an additional carbon source. High performance liquid chromatography and gas chromatography-mass spectrometry detected indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde as biotransformation products. This is the first report of the formation of indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde from indole by any bacterium.

No MeSH data available.


Related in: MedlinePlus