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Antibacterial properties of Acinetobacter baumannii phage Abp1 endolysin (PlyAB1).

Huang G, Shen X, Gong Y, Dong Z, Zhao X, Shen W, Wang J, Hu F, Peng Y - BMC Infect. Dis. (2014)

Bottom Line: Acinetobacter baumannii has emerged as one of the most important hospital-acquired pathogens in the world, because of its resistance to almost all available antibiotic drugs.These isolates were shown to belong to different ST clones by multilocus sequence typing.The results presented here show that PlyAB1 has potential as an antibiotic against drug-resistant A. baumannii.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China. haitao3140@sina.com.

ABSTRACT

Background: Acinetobacter baumannii has emerged as one of the most important hospital-acquired pathogens in the world, because of its resistance to almost all available antibiotic drugs. Endolysins from phages are attracting increasing interest as potential antimicrobial agents, especially for drug-resistant bacteria. We previously isolated and characterized Abp1, a virulent phage targeting the multidrug-resistant A. baumannii strain, AB1.

Methods: To evaluate the antimicrobial potential of endolysin from the Abp1 phage, the endolysin gene plyAB1 was cloned and over-expressed in Escherichia coli, and the lytic activity of the recombinant protein (PlyAB1) was tested by turbidity assessment and bacteria counting assays.

Results: PlyAB1 exhibits a marked lytic activity against A. baumannii AB1, as shown by a decrease in the number of live bacteria following treatment with the enzyme. Moreover, PlyAB1 displayed a highly specific lytic effect against all of the 48 hospital-derived pandrug-resistant A. baumannii isolates that were tested. These isolates were shown to belong to different ST clones by multilocus sequence typing.

Conclusions: The results presented here show that PlyAB1 has potential as an antibiotic against drug-resistant A. baumannii.

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Related in: MedlinePlus

Characterization of clinical pandrug-resistantA. baumanniiisolates (PDRAB). The 48 PDRAB isolates are grouped into seven different MLST types.
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Fig4: Characterization of clinical pandrug-resistantA. baumanniiisolates (PDRAB). The 48 PDRAB isolates are grouped into seven different MLST types.

Mentions: The blaOXA-51-like gene is a species-specific gene of A. baumannii and was used here for identification. The 48 A. baumannii isolates were all found to harbor this gene. Of the 19 antibiotics tested, all 48 of the clinical strains were only sensitive to polymyxin B. However, they showed general resistance to cephalosporins, carbapenems, tetracyclines, aminoglycosides, sulfonamides, and quinolones. By definition, this shows all of the clinical A. baumannii strains are PDRAB. The PDRAB isolates could be grouped into seven existing MLST types (FigureĀ 4). ST368 dominated, while 27 isolates belonged to ST368. Other MLST types included ST369 (8), ST191 (4), ST195 (4), ST208 (3), ST381 (1), and ST205 (1).Figure 4


Antibacterial properties of Acinetobacter baumannii phage Abp1 endolysin (PlyAB1).

Huang G, Shen X, Gong Y, Dong Z, Zhao X, Shen W, Wang J, Hu F, Peng Y - BMC Infect. Dis. (2014)

Characterization of clinical pandrug-resistantA. baumanniiisolates (PDRAB). The 48 PDRAB isolates are grouped into seven different MLST types.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4274762&req=5

Fig4: Characterization of clinical pandrug-resistantA. baumanniiisolates (PDRAB). The 48 PDRAB isolates are grouped into seven different MLST types.
Mentions: The blaOXA-51-like gene is a species-specific gene of A. baumannii and was used here for identification. The 48 A. baumannii isolates were all found to harbor this gene. Of the 19 antibiotics tested, all 48 of the clinical strains were only sensitive to polymyxin B. However, they showed general resistance to cephalosporins, carbapenems, tetracyclines, aminoglycosides, sulfonamides, and quinolones. By definition, this shows all of the clinical A. baumannii strains are PDRAB. The PDRAB isolates could be grouped into seven existing MLST types (FigureĀ 4). ST368 dominated, while 27 isolates belonged to ST368. Other MLST types included ST369 (8), ST191 (4), ST195 (4), ST208 (3), ST381 (1), and ST205 (1).Figure 4

Bottom Line: Acinetobacter baumannii has emerged as one of the most important hospital-acquired pathogens in the world, because of its resistance to almost all available antibiotic drugs.These isolates were shown to belong to different ST clones by multilocus sequence typing.The results presented here show that PlyAB1 has potential as an antibiotic against drug-resistant A. baumannii.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China. haitao3140@sina.com.

ABSTRACT

Background: Acinetobacter baumannii has emerged as one of the most important hospital-acquired pathogens in the world, because of its resistance to almost all available antibiotic drugs. Endolysins from phages are attracting increasing interest as potential antimicrobial agents, especially for drug-resistant bacteria. We previously isolated and characterized Abp1, a virulent phage targeting the multidrug-resistant A. baumannii strain, AB1.

Methods: To evaluate the antimicrobial potential of endolysin from the Abp1 phage, the endolysin gene plyAB1 was cloned and over-expressed in Escherichia coli, and the lytic activity of the recombinant protein (PlyAB1) was tested by turbidity assessment and bacteria counting assays.

Results: PlyAB1 exhibits a marked lytic activity against A. baumannii AB1, as shown by a decrease in the number of live bacteria following treatment with the enzyme. Moreover, PlyAB1 displayed a highly specific lytic effect against all of the 48 hospital-derived pandrug-resistant A. baumannii isolates that were tested. These isolates were shown to belong to different ST clones by multilocus sequence typing.

Conclusions: The results presented here show that PlyAB1 has potential as an antibiotic against drug-resistant A. baumannii.

Show MeSH
Related in: MedlinePlus