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Antibacterial properties of Acinetobacter baumannii phage Abp1 endolysin (PlyAB1).

Huang G, Shen X, Gong Y, Dong Z, Zhao X, Shen W, Wang J, Hu F, Peng Y - BMC Infect. Dis. (2014)

Bottom Line: Acinetobacter baumannii has emerged as one of the most important hospital-acquired pathogens in the world, because of its resistance to almost all available antibiotic drugs.These isolates were shown to belong to different ST clones by multilocus sequence typing.The results presented here show that PlyAB1 has potential as an antibiotic against drug-resistant A. baumannii.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China. haitao3140@sina.com.

ABSTRACT

Background: Acinetobacter baumannii has emerged as one of the most important hospital-acquired pathogens in the world, because of its resistance to almost all available antibiotic drugs. Endolysins from phages are attracting increasing interest as potential antimicrobial agents, especially for drug-resistant bacteria. We previously isolated and characterized Abp1, a virulent phage targeting the multidrug-resistant A. baumannii strain, AB1.

Methods: To evaluate the antimicrobial potential of endolysin from the Abp1 phage, the endolysin gene plyAB1 was cloned and over-expressed in Escherichia coli, and the lytic activity of the recombinant protein (PlyAB1) was tested by turbidity assessment and bacteria counting assays.

Results: PlyAB1 exhibits a marked lytic activity against A. baumannii AB1, as shown by a decrease in the number of live bacteria following treatment with the enzyme. Moreover, PlyAB1 displayed a highly specific lytic effect against all of the 48 hospital-derived pandrug-resistant A. baumannii isolates that were tested. These isolates were shown to belong to different ST clones by multilocus sequence typing.

Conclusions: The results presented here show that PlyAB1 has potential as an antibiotic against drug-resistant A. baumannii.

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Lytic activity of PlyAB1 against AB1. (A) Lytic activity was determined by OD600 measurements. (B) The concentration of live bacteria decreased from 1.4 × 107 CFU/ml at 0 min to 4.1 × 106 CFU/ml at 30 min.
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Fig3: Lytic activity of PlyAB1 against AB1. (A) Lytic activity was determined by OD600 measurements. (B) The concentration of live bacteria decreased from 1.4 × 107 CFU/ml at 0 min to 4.1 × 106 CFU/ml at 30 min.

Mentions: In Hepes/KOH buffer, A. baumannii AB1 was lysed effectively by the purified PlyAB1 protein. Within 30 min, the OD600 of the reaction between AB1 and PlyAB1 fell from 1.05 to 0.2 (Figure 3A). However, in the negative control group, the OD600 values of the AB1 and reaction buffer mixture showed almost no reduction. The viable bacterial counts results showed that the number of A. baumannii AB1 decreased from 1.4 × 107 CFU/ml to 4.1 × 106 CFU/ml in 30 minutes with 100 μg/ml of PlyAB1 (Figure 3B). The viable bacterial counts decreased by 82.6%.Figure 3


Antibacterial properties of Acinetobacter baumannii phage Abp1 endolysin (PlyAB1).

Huang G, Shen X, Gong Y, Dong Z, Zhao X, Shen W, Wang J, Hu F, Peng Y - BMC Infect. Dis. (2014)

Lytic activity of PlyAB1 against AB1. (A) Lytic activity was determined by OD600 measurements. (B) The concentration of live bacteria decreased from 1.4 × 107 CFU/ml at 0 min to 4.1 × 106 CFU/ml at 30 min.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4274762&req=5

Fig3: Lytic activity of PlyAB1 against AB1. (A) Lytic activity was determined by OD600 measurements. (B) The concentration of live bacteria decreased from 1.4 × 107 CFU/ml at 0 min to 4.1 × 106 CFU/ml at 30 min.
Mentions: In Hepes/KOH buffer, A. baumannii AB1 was lysed effectively by the purified PlyAB1 protein. Within 30 min, the OD600 of the reaction between AB1 and PlyAB1 fell from 1.05 to 0.2 (Figure 3A). However, in the negative control group, the OD600 values of the AB1 and reaction buffer mixture showed almost no reduction. The viable bacterial counts results showed that the number of A. baumannii AB1 decreased from 1.4 × 107 CFU/ml to 4.1 × 106 CFU/ml in 30 minutes with 100 μg/ml of PlyAB1 (Figure 3B). The viable bacterial counts decreased by 82.6%.Figure 3

Bottom Line: Acinetobacter baumannii has emerged as one of the most important hospital-acquired pathogens in the world, because of its resistance to almost all available antibiotic drugs.These isolates were shown to belong to different ST clones by multilocus sequence typing.The results presented here show that PlyAB1 has potential as an antibiotic against drug-resistant A. baumannii.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing, China. haitao3140@sina.com.

ABSTRACT

Background: Acinetobacter baumannii has emerged as one of the most important hospital-acquired pathogens in the world, because of its resistance to almost all available antibiotic drugs. Endolysins from phages are attracting increasing interest as potential antimicrobial agents, especially for drug-resistant bacteria. We previously isolated and characterized Abp1, a virulent phage targeting the multidrug-resistant A. baumannii strain, AB1.

Methods: To evaluate the antimicrobial potential of endolysin from the Abp1 phage, the endolysin gene plyAB1 was cloned and over-expressed in Escherichia coli, and the lytic activity of the recombinant protein (PlyAB1) was tested by turbidity assessment and bacteria counting assays.

Results: PlyAB1 exhibits a marked lytic activity against A. baumannii AB1, as shown by a decrease in the number of live bacteria following treatment with the enzyme. Moreover, PlyAB1 displayed a highly specific lytic effect against all of the 48 hospital-derived pandrug-resistant A. baumannii isolates that were tested. These isolates were shown to belong to different ST clones by multilocus sequence typing.

Conclusions: The results presented here show that PlyAB1 has potential as an antibiotic against drug-resistant A. baumannii.

Show MeSH
Related in: MedlinePlus