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Molecular characterization of cryptic species of Anopheles barbirostris van der Wulp in China.

Wang Y, Xu J, Ma Y - Parasit Vectors (2014)

Bottom Line: The sequence comparison revealed two cryptic species, corresponding to An. barbirostris A1/clade III and A2/clade IV, respectively.The molecular characterization updated the species composition of the An. barbirostris complex in China.This study distinguished two molecular forms in the An. barbirostris s. l. in China.

View Article: PubMed Central - PubMed

Affiliation: Department of Tropical Infectious Diseases, Second Military Medical University, 800 Xiangyin Rd, Shanghai, 200433, China. wang1yu2yan@126.com.

ABSTRACT

Background: Anopheles barbirostris sensu lato belongs to the Barbirostris subgroup of the subgenus Anopheles that is distributed in Southeast Asia. Different molecular forms have been identified based on the rDNA-ITS2 and mtDNA-COI sequences. Anopheles barbirostris occurs in China. The species status was uncertain due to the lack of molecular characterization. The present study characterized Chinese An. barbirostris using rDNA-ITS2 and mtDNA-COI gene sequences. Two cryptic species were identified.

Findings: DNA was extracted from morphologically identified An. barbirostris specimens collected in Yunnan and Hainan from China, the sequences of rDNA-ITS2 and mtDNA-COI regions of 40 individuals were amplified and analyzed. The sequence comparison revealed two cryptic species, corresponding to An. barbirostris A1/clade III and A2/clade IV, respectively. The molecular characterization updated the species composition of the An. barbirostris complex in China.

Conclusions: This study distinguished two molecular forms in the An. barbirostris s. l. in China.

Show MeSH
Maximum likelihood tree created from COI sequences with the HKY + I substitution model. The referential sequences were clade III (EU797221) and clade IV (EU797257). The bootstrap values are shown at nodes.
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Fig2: Maximum likelihood tree created from COI sequences with the HKY + I substitution model. The referential sequences were clade III (EU797221) and clade IV (EU797257). The bootstrap values are shown at nodes.

Mentions: A 830 bp fragment of the COI gene was used for the analysis in this study. Out of 40 specimens, 38 sequences were almost identical (99-100% identity) to that of the An. barbirostris clade III (EU797221). The remaining two sequences CM5 and CM6 were the same as the An. barbirostris clade IV (EU797257). The intraspecific variation (p-distance) of mtDNA-COI gene sequences was below 0.012, and interspecific variation between An. barbirostris clade III with IV was greater than 0.031. The ML tree using COI sequences was reconstructed by HKY + I model (Figure 2). The specimens were clustered into two clades, corresponding to the An. barbirostris clade III and clade IV, respectively. This is consistent with the findings of the ITS2 analysis.Figure 2


Molecular characterization of cryptic species of Anopheles barbirostris van der Wulp in China.

Wang Y, Xu J, Ma Y - Parasit Vectors (2014)

Maximum likelihood tree created from COI sequences with the HKY + I substitution model. The referential sequences were clade III (EU797221) and clade IV (EU797257). The bootstrap values are shown at nodes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4274727&req=5

Fig2: Maximum likelihood tree created from COI sequences with the HKY + I substitution model. The referential sequences were clade III (EU797221) and clade IV (EU797257). The bootstrap values are shown at nodes.
Mentions: A 830 bp fragment of the COI gene was used for the analysis in this study. Out of 40 specimens, 38 sequences were almost identical (99-100% identity) to that of the An. barbirostris clade III (EU797221). The remaining two sequences CM5 and CM6 were the same as the An. barbirostris clade IV (EU797257). The intraspecific variation (p-distance) of mtDNA-COI gene sequences was below 0.012, and interspecific variation between An. barbirostris clade III with IV was greater than 0.031. The ML tree using COI sequences was reconstructed by HKY + I model (Figure 2). The specimens were clustered into two clades, corresponding to the An. barbirostris clade III and clade IV, respectively. This is consistent with the findings of the ITS2 analysis.Figure 2

Bottom Line: The sequence comparison revealed two cryptic species, corresponding to An. barbirostris A1/clade III and A2/clade IV, respectively.The molecular characterization updated the species composition of the An. barbirostris complex in China.This study distinguished two molecular forms in the An. barbirostris s. l. in China.

View Article: PubMed Central - PubMed

Affiliation: Department of Tropical Infectious Diseases, Second Military Medical University, 800 Xiangyin Rd, Shanghai, 200433, China. wang1yu2yan@126.com.

ABSTRACT

Background: Anopheles barbirostris sensu lato belongs to the Barbirostris subgroup of the subgenus Anopheles that is distributed in Southeast Asia. Different molecular forms have been identified based on the rDNA-ITS2 and mtDNA-COI sequences. Anopheles barbirostris occurs in China. The species status was uncertain due to the lack of molecular characterization. The present study characterized Chinese An. barbirostris using rDNA-ITS2 and mtDNA-COI gene sequences. Two cryptic species were identified.

Findings: DNA was extracted from morphologically identified An. barbirostris specimens collected in Yunnan and Hainan from China, the sequences of rDNA-ITS2 and mtDNA-COI regions of 40 individuals were amplified and analyzed. The sequence comparison revealed two cryptic species, corresponding to An. barbirostris A1/clade III and A2/clade IV, respectively. The molecular characterization updated the species composition of the An. barbirostris complex in China.

Conclusions: This study distinguished two molecular forms in the An. barbirostris s. l. in China.

Show MeSH