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Molecular characterization of cryptic species of Anopheles barbirostris van der Wulp in China.

Wang Y, Xu J, Ma Y - Parasit Vectors (2014)

Bottom Line: The sequence comparison revealed two cryptic species, corresponding to An. barbirostris A1/clade III and A2/clade IV, respectively.The molecular characterization updated the species composition of the An. barbirostris complex in China.This study distinguished two molecular forms in the An. barbirostris s. l. in China.

View Article: PubMed Central - PubMed

Affiliation: Department of Tropical Infectious Diseases, Second Military Medical University, 800 Xiangyin Rd, Shanghai, 200433, China. wang1yu2yan@126.com.

ABSTRACT

Background: Anopheles barbirostris sensu lato belongs to the Barbirostris subgroup of the subgenus Anopheles that is distributed in Southeast Asia. Different molecular forms have been identified based on the rDNA-ITS2 and mtDNA-COI sequences. Anopheles barbirostris occurs in China. The species status was uncertain due to the lack of molecular characterization. The present study characterized Chinese An. barbirostris using rDNA-ITS2 and mtDNA-COI gene sequences. Two cryptic species were identified.

Findings: DNA was extracted from morphologically identified An. barbirostris specimens collected in Yunnan and Hainan from China, the sequences of rDNA-ITS2 and mtDNA-COI regions of 40 individuals were amplified and analyzed. The sequence comparison revealed two cryptic species, corresponding to An. barbirostris A1/clade III and A2/clade IV, respectively. The molecular characterization updated the species composition of the An. barbirostris complex in China.

Conclusions: This study distinguished two molecular forms in the An. barbirostris s. l. in China.

Show MeSH
Maximum likelihood tree created from ITS2 sequences with the GTR substitution model. The referential sequences were A1 (AB331555) and clade III (EU812782), A2 (AB331551) and clade IV (EU812794). The bootstrap values were shown at nodes.
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Fig1: Maximum likelihood tree created from ITS2 sequences with the GTR substitution model. The referential sequences were A1 (AB331555) and clade III (EU812782), A2 (AB331551) and clade IV (EU812794). The bootstrap values were shown at nodes.

Mentions: The relationships of the specimens were further analyzed by maximum likelihood. Different models were tested for making a ML tree. FigureĀ 1 presented a ML tree that was reconstructed by GTR model. Topology of the tree showed two clades, corresponding to An. barbirostris A1/clade III and A2/clade IV, respectively. This clearly indicates that there are at least two molecular forms in the An. barbirostris complex in China.Figure 1


Molecular characterization of cryptic species of Anopheles barbirostris van der Wulp in China.

Wang Y, Xu J, Ma Y - Parasit Vectors (2014)

Maximum likelihood tree created from ITS2 sequences with the GTR substitution model. The referential sequences were A1 (AB331555) and clade III (EU812782), A2 (AB331551) and clade IV (EU812794). The bootstrap values were shown at nodes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4274727&req=5

Fig1: Maximum likelihood tree created from ITS2 sequences with the GTR substitution model. The referential sequences were A1 (AB331555) and clade III (EU812782), A2 (AB331551) and clade IV (EU812794). The bootstrap values were shown at nodes.
Mentions: The relationships of the specimens were further analyzed by maximum likelihood. Different models were tested for making a ML tree. FigureĀ 1 presented a ML tree that was reconstructed by GTR model. Topology of the tree showed two clades, corresponding to An. barbirostris A1/clade III and A2/clade IV, respectively. This clearly indicates that there are at least two molecular forms in the An. barbirostris complex in China.Figure 1

Bottom Line: The sequence comparison revealed two cryptic species, corresponding to An. barbirostris A1/clade III and A2/clade IV, respectively.The molecular characterization updated the species composition of the An. barbirostris complex in China.This study distinguished two molecular forms in the An. barbirostris s. l. in China.

View Article: PubMed Central - PubMed

Affiliation: Department of Tropical Infectious Diseases, Second Military Medical University, 800 Xiangyin Rd, Shanghai, 200433, China. wang1yu2yan@126.com.

ABSTRACT

Background: Anopheles barbirostris sensu lato belongs to the Barbirostris subgroup of the subgenus Anopheles that is distributed in Southeast Asia. Different molecular forms have been identified based on the rDNA-ITS2 and mtDNA-COI sequences. Anopheles barbirostris occurs in China. The species status was uncertain due to the lack of molecular characterization. The present study characterized Chinese An. barbirostris using rDNA-ITS2 and mtDNA-COI gene sequences. Two cryptic species were identified.

Findings: DNA was extracted from morphologically identified An. barbirostris specimens collected in Yunnan and Hainan from China, the sequences of rDNA-ITS2 and mtDNA-COI regions of 40 individuals were amplified and analyzed. The sequence comparison revealed two cryptic species, corresponding to An. barbirostris A1/clade III and A2/clade IV, respectively. The molecular characterization updated the species composition of the An. barbirostris complex in China.

Conclusions: This study distinguished two molecular forms in the An. barbirostris s. l. in China.

Show MeSH