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Setae from larvae of the northern processionary moth (Thaumetopoea pinivora, TP) stimulate proliferation of human blood lymphocytes in vitro.

Holm G, Andersson M, Ekberg M, Fagrell B, Sjöberg J, Bottai M, Björkholm M - PLoS ONE (2014)

Bottom Line: Lymphoproliferation was measured as uptake of 3H-thymidine.In conclusion, setae contain molecules that in the presence of monocytes activate human T-lymphocytes to proliferation.The nature of such molecules remains to be defined.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, Division of Hematology, Center for Molecular Medicine, Karolinska University Hospital Solna and Karolinska Institutet, Stockholm, Sweden.

ABSTRACT
Larvae of the Northern pine processionary moth (Thaumetopoea pinivora, TP) carry microscopic needles (setae), which by penetrating skin and mucous membranes, may cause inflammatory/immune derived symptoms in man. In the present study the stimulatory effects of setae on human blood lymphocytes in vitro was investigated. Blood mononuclear cells were separated from venous blood or buffy coat of ten healthy individuals, six previously exposed to setae and four with no known exposure. Lymphoproliferation was measured as uptake of 3H-thymidine. Setae were prepared from TP larvae. Setae and saline setae extracts stimulated proliferation of T-lymphocytes in the presence of monocytic cells. Stimulation was pronounced in cells from persons who had been exposed to setae, and weak in cells from non-exposed donors. Chitin also induced lymphocyte proliferation in most donors, but to a lesser extent and independently of donor's previous exposure to setae. In conclusion, setae contain molecules that in the presence of monocytes activate human T-lymphocytes to proliferation. The antigenic nature of stimulatory molecules was supported by the significantly stronger lymphocyte response in persons previously exposed to setae than in non-exposed donors. The nature of such molecules remains to be defined.

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Related in: MedlinePlus

Cytospin preparations of PBMC after 6 days of incubation.A and B represents a donor earlier exposed to setae, C and D illustrates not earlier exposed donors. The microscopic view indicated no difference between exposed and non-exposed donors. In A and C mononuclear are seen cells attached to the setae B and D the shows patterns of disintegrated seta. The cultured PBMC and setae were collected by cytospin and stained with May-Grunwald Giemsa.
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pone-0113977-g003: Cytospin preparations of PBMC after 6 days of incubation.A and B represents a donor earlier exposed to setae, C and D illustrates not earlier exposed donors. The microscopic view indicated no difference between exposed and non-exposed donors. In A and C mononuclear are seen cells attached to the setae B and D the shows patterns of disintegrated seta. The cultured PBMC and setae were collected by cytospin and stained with May-Grunwald Giemsa.

Mentions: Using phase contrast microscopy mononuclear cells were seen to adhere to setae within 24 hours of culture and some cells are stretched along the setae (Fig. 1) that had been prepared from setae mirrors of the larvae (Fig. 2A). No damage of setae was noted at this time of incubation. As observed in stained cytospin preparations, some of the cells seemed to adhere to setae via cellular protrusions. After 72 hours of culture, large cells with pale and large nuclei and irregular cytoplasms with protrusions were seen in close contact with setae (Fig. 2C). Such setae often showed signs of disintegration (Fig. 2C). Fig. 3 illustrates the interaction between setae and PBMC from a donor earlier exposed for setae (A and B) as well as from donors never exposed to setae (C and D) after 6 days of incubation. The microscopy images showed a similar pattern of adhesion and setae damage independent of whether the donors had been exposed to setae or not (Fig. 3). In summary, mononuclear cells adhere to setae within 24 hours of incubation. Damage of seta was first observed after 72 hours of culture and proceed to disintegration of the majority of setae after 6 days.


Setae from larvae of the northern processionary moth (Thaumetopoea pinivora, TP) stimulate proliferation of human blood lymphocytes in vitro.

Holm G, Andersson M, Ekberg M, Fagrell B, Sjöberg J, Bottai M, Björkholm M - PLoS ONE (2014)

Cytospin preparations of PBMC after 6 days of incubation.A and B represents a donor earlier exposed to setae, C and D illustrates not earlier exposed donors. The microscopic view indicated no difference between exposed and non-exposed donors. In A and C mononuclear are seen cells attached to the setae B and D the shows patterns of disintegrated seta. The cultured PBMC and setae were collected by cytospin and stained with May-Grunwald Giemsa.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4273998&req=5

pone-0113977-g003: Cytospin preparations of PBMC after 6 days of incubation.A and B represents a donor earlier exposed to setae, C and D illustrates not earlier exposed donors. The microscopic view indicated no difference between exposed and non-exposed donors. In A and C mononuclear are seen cells attached to the setae B and D the shows patterns of disintegrated seta. The cultured PBMC and setae were collected by cytospin and stained with May-Grunwald Giemsa.
Mentions: Using phase contrast microscopy mononuclear cells were seen to adhere to setae within 24 hours of culture and some cells are stretched along the setae (Fig. 1) that had been prepared from setae mirrors of the larvae (Fig. 2A). No damage of setae was noted at this time of incubation. As observed in stained cytospin preparations, some of the cells seemed to adhere to setae via cellular protrusions. After 72 hours of culture, large cells with pale and large nuclei and irregular cytoplasms with protrusions were seen in close contact with setae (Fig. 2C). Such setae often showed signs of disintegration (Fig. 2C). Fig. 3 illustrates the interaction between setae and PBMC from a donor earlier exposed for setae (A and B) as well as from donors never exposed to setae (C and D) after 6 days of incubation. The microscopy images showed a similar pattern of adhesion and setae damage independent of whether the donors had been exposed to setae or not (Fig. 3). In summary, mononuclear cells adhere to setae within 24 hours of incubation. Damage of seta was first observed after 72 hours of culture and proceed to disintegration of the majority of setae after 6 days.

Bottom Line: Lymphoproliferation was measured as uptake of 3H-thymidine.In conclusion, setae contain molecules that in the presence of monocytes activate human T-lymphocytes to proliferation.The nature of such molecules remains to be defined.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine, Division of Hematology, Center for Molecular Medicine, Karolinska University Hospital Solna and Karolinska Institutet, Stockholm, Sweden.

ABSTRACT
Larvae of the Northern pine processionary moth (Thaumetopoea pinivora, TP) carry microscopic needles (setae), which by penetrating skin and mucous membranes, may cause inflammatory/immune derived symptoms in man. In the present study the stimulatory effects of setae on human blood lymphocytes in vitro was investigated. Blood mononuclear cells were separated from venous blood or buffy coat of ten healthy individuals, six previously exposed to setae and four with no known exposure. Lymphoproliferation was measured as uptake of 3H-thymidine. Setae were prepared from TP larvae. Setae and saline setae extracts stimulated proliferation of T-lymphocytes in the presence of monocytic cells. Stimulation was pronounced in cells from persons who had been exposed to setae, and weak in cells from non-exposed donors. Chitin also induced lymphocyte proliferation in most donors, but to a lesser extent and independently of donor's previous exposure to setae. In conclusion, setae contain molecules that in the presence of monocytes activate human T-lymphocytes to proliferation. The antigenic nature of stimulatory molecules was supported by the significantly stronger lymphocyte response in persons previously exposed to setae than in non-exposed donors. The nature of such molecules remains to be defined.

Show MeSH
Related in: MedlinePlus