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Effects of subchronic exposure to cadmium and diazinon on testis and epididymis in rats.

Adamkovicova M, Toman R, Cabaj M, Massanyi P, Martiniakova M, Omelka R, Krajcovicova V, Duranova H - ScientificWorldJournal (2014)

Bottom Line: Desquamation of immature germ cells resulted in a significant increase of intraepithelial spaces and reduced tubule volume in all experimental groups.Results did not indicate a synergistic or any additional effect from the simultaneous administration of both toxicants.Further research is needed to determine the significance and the mechanism of the adverse effects.

View Article: PubMed Central - PubMed

Affiliation: Department of Botany and Genetics, Constantine the Philosopher University, Nitra 949 74, Slovakia.

ABSTRACT
The present study aimed to elucidate the structural changes in testis and epididymis of adult rats following subchronic peroral administration of cadmium at 30 mg/L, diazinon at 40 mg/L, cadmium at 30 mg/L, and diazinon at 40 mg/L, respectively. At the end of 90-day experiment, the samples of the testes and epididymis were assayed by qualitative and quantitative histological methods. The testis and epididymis weights increased following exposure to cadmium and simultaneous exposure to cadmium and diazinon. Testicular damage following cadmium and diazinon coexposure was significantly less expressive than in groups with individual administration of these compounds. Cadmium caused a significant thickening of seminiferous epithelium, cellular degeneration, and necrosis. Desquamation of immature germ cells resulted in a significant increase of intraepithelial spaces and reduced tubule volume in all experimental groups. Vascular dilation and congestion were detected in the interstitial tissue. The changes in epididymal histology in the group exposed to cadmium and group exposed simultaneously included a reduction of epithelium, necrotic epithelial cells, vasoconstriction, and interstitial edema together with mononuclear cell infiltration. Results did not indicate a synergistic or any additional effect from the simultaneous administration of both toxicants. Further research is needed to determine the significance and the mechanism of the adverse effects.

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Related in: MedlinePlus

Photomicrographs showing histology of epididymal tissue from control group (a), group exposed to cadmium at a dose of 30 mg/L (b), group exposed to diazinon at a dose of 40 mg/L (c), and group exposed to cadmium at a dose of 30 mg/L and diazinon at a dose of 40 mg/L (d). ET: epididymal tubules, PE: pseudostratified columnar epithelium, IT: interstitial tissue, SM: spermatozoa, BV: blood vessels, CD: cellular debris, and arrows: intraepithelial empty spaces (hematoxylin-eosin, original magnification ×200).
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fig2: Photomicrographs showing histology of epididymal tissue from control group (a), group exposed to cadmium at a dose of 30 mg/L (b), group exposed to diazinon at a dose of 40 mg/L (c), and group exposed to cadmium at a dose of 30 mg/L and diazinon at a dose of 40 mg/L (d). ET: epididymal tubules, PE: pseudostratified columnar epithelium, IT: interstitial tissue, SM: spermatozoa, BV: blood vessels, CD: cellular debris, and arrows: intraepithelial empty spaces (hematoxylin-eosin, original magnification ×200).

Mentions: The cross sections of cauda epididymis obtained from the control group revealed regular and circular tubules with a pseudostratified columnar epithelium that exhibits stereocilia. A dense collection of sperm cells in the tubular lumen was evident. Extratubular space contained blood vessels in the interstitial connective tissue. The epithelium was separated from the connective tissue by an intact basement membrane (Figure 2(a)). Histological analysis did not reveal differences in epididymal morphology of rats, except for the vacuole formation within pseudostratified epithelium following cadmium (Figure 2(b)) and combined cadmium and diazinon exposure in which moreover an enlargement in the interstitial space was found (Figure 2(d)). No marked alteration in epididymal structural integrity was observed in rats after diazinon exposure (Figure 2(c)).


Effects of subchronic exposure to cadmium and diazinon on testis and epididymis in rats.

Adamkovicova M, Toman R, Cabaj M, Massanyi P, Martiniakova M, Omelka R, Krajcovicova V, Duranova H - ScientificWorldJournal (2014)

Photomicrographs showing histology of epididymal tissue from control group (a), group exposed to cadmium at a dose of 30 mg/L (b), group exposed to diazinon at a dose of 40 mg/L (c), and group exposed to cadmium at a dose of 30 mg/L and diazinon at a dose of 40 mg/L (d). ET: epididymal tubules, PE: pseudostratified columnar epithelium, IT: interstitial tissue, SM: spermatozoa, BV: blood vessels, CD: cellular debris, and arrows: intraepithelial empty spaces (hematoxylin-eosin, original magnification ×200).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4273512&req=5

fig2: Photomicrographs showing histology of epididymal tissue from control group (a), group exposed to cadmium at a dose of 30 mg/L (b), group exposed to diazinon at a dose of 40 mg/L (c), and group exposed to cadmium at a dose of 30 mg/L and diazinon at a dose of 40 mg/L (d). ET: epididymal tubules, PE: pseudostratified columnar epithelium, IT: interstitial tissue, SM: spermatozoa, BV: blood vessels, CD: cellular debris, and arrows: intraepithelial empty spaces (hematoxylin-eosin, original magnification ×200).
Mentions: The cross sections of cauda epididymis obtained from the control group revealed regular and circular tubules with a pseudostratified columnar epithelium that exhibits stereocilia. A dense collection of sperm cells in the tubular lumen was evident. Extratubular space contained blood vessels in the interstitial connective tissue. The epithelium was separated from the connective tissue by an intact basement membrane (Figure 2(a)). Histological analysis did not reveal differences in epididymal morphology of rats, except for the vacuole formation within pseudostratified epithelium following cadmium (Figure 2(b)) and combined cadmium and diazinon exposure in which moreover an enlargement in the interstitial space was found (Figure 2(d)). No marked alteration in epididymal structural integrity was observed in rats after diazinon exposure (Figure 2(c)).

Bottom Line: Desquamation of immature germ cells resulted in a significant increase of intraepithelial spaces and reduced tubule volume in all experimental groups.Results did not indicate a synergistic or any additional effect from the simultaneous administration of both toxicants.Further research is needed to determine the significance and the mechanism of the adverse effects.

View Article: PubMed Central - PubMed

Affiliation: Department of Botany and Genetics, Constantine the Philosopher University, Nitra 949 74, Slovakia.

ABSTRACT
The present study aimed to elucidate the structural changes in testis and epididymis of adult rats following subchronic peroral administration of cadmium at 30 mg/L, diazinon at 40 mg/L, cadmium at 30 mg/L, and diazinon at 40 mg/L, respectively. At the end of 90-day experiment, the samples of the testes and epididymis were assayed by qualitative and quantitative histological methods. The testis and epididymis weights increased following exposure to cadmium and simultaneous exposure to cadmium and diazinon. Testicular damage following cadmium and diazinon coexposure was significantly less expressive than in groups with individual administration of these compounds. Cadmium caused a significant thickening of seminiferous epithelium, cellular degeneration, and necrosis. Desquamation of immature germ cells resulted in a significant increase of intraepithelial spaces and reduced tubule volume in all experimental groups. Vascular dilation and congestion were detected in the interstitial tissue. The changes in epididymal histology in the group exposed to cadmium and group exposed simultaneously included a reduction of epithelium, necrotic epithelial cells, vasoconstriction, and interstitial edema together with mononuclear cell infiltration. Results did not indicate a synergistic or any additional effect from the simultaneous administration of both toxicants. Further research is needed to determine the significance and the mechanism of the adverse effects.

Show MeSH
Related in: MedlinePlus