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Homeostasis in C. elegans sleep is characterized by two behaviorally and genetically distinct mechanisms.

Nagy S, Tramm N, Sanders J, Iwanir S, Shirley IA, Levine E, Biron D - Elife (2014)

Bottom Line: This response to strong stimuli required the function of the DAF-16/FOXO transcription factor in neurons, but not that of NPR-1.Conversely, response to weak stimuli did not require the function of DAF-16/FOXO.These findings suggest that routine homeostatic stabilization of sleep may be distinct from homeostatic compensation following a strong disturbance.

View Article: PubMed Central - PubMed

Affiliation: Institute for Biophysical Dynamics, University of Chicago, Chicago, United States.

ABSTRACT
Biological homeostasis invokes modulatory responses aimed at stabilizing internal conditions. Using tunable photo- and mechano-stimulation, we identified two distinct categories of homeostatic responses during the sleep-like state of Caenorhabditis elegans (lethargus). In the presence of weak or no stimuli, extended motion caused a subsequent extension of quiescence. The neuropeptide Y receptor homolog, NPR-1, and an inhibitory neuropeptide known to activate it, FLP-18, were required for this process. In the presence of strong stimuli, the correlations between motion and quiescence were disrupted for several minutes but homeostasis manifested as an overall elevation of the time spent in quiescence. This response to strong stimuli required the function of the DAF-16/FOXO transcription factor in neurons, but not that of NPR-1. Conversely, response to weak stimuli did not require the function of DAF-16/FOXO. These findings suggest that routine homeostatic stabilization of sleep may be distinct from homeostatic compensation following a strong disturbance.

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Micro-homeostasis in undisturbed Hawaiian wild-isolates.The durations of bouts of quiescence, as well as pairwise correlations, were measured for Hawaiian animals and unc-31 mutants as a function of time during L4 lethargus. The detailed comparison reveals that the duration of bouts cannot in and of themselves account for the strength of the pairwise correlations (see Figure 7A for details).DOI:http://dx.doi.org/10.7554/eLife.04380.014
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fig6s2: Micro-homeostasis in undisturbed Hawaiian wild-isolates.The durations of bouts of quiescence, as well as pairwise correlations, were measured for Hawaiian animals and unc-31 mutants as a function of time during L4 lethargus. The detailed comparison reveals that the duration of bouts cannot in and of themselves account for the strength of the pairwise correlations (see Figure 7A for details).DOI:http://dx.doi.org/10.7554/eLife.04380.014


Homeostasis in C. elegans sleep is characterized by two behaviorally and genetically distinct mechanisms.

Nagy S, Tramm N, Sanders J, Iwanir S, Shirley IA, Levine E, Biron D - Elife (2014)

Micro-homeostasis in undisturbed Hawaiian wild-isolates.The durations of bouts of quiescence, as well as pairwise correlations, were measured for Hawaiian animals and unc-31 mutants as a function of time during L4 lethargus. The detailed comparison reveals that the duration of bouts cannot in and of themselves account for the strength of the pairwise correlations (see Figure 7A for details).DOI:http://dx.doi.org/10.7554/eLife.04380.014
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4273442&req=5

fig6s2: Micro-homeostasis in undisturbed Hawaiian wild-isolates.The durations of bouts of quiescence, as well as pairwise correlations, were measured for Hawaiian animals and unc-31 mutants as a function of time during L4 lethargus. The detailed comparison reveals that the duration of bouts cannot in and of themselves account for the strength of the pairwise correlations (see Figure 7A for details).DOI:http://dx.doi.org/10.7554/eLife.04380.014
Bottom Line: This response to strong stimuli required the function of the DAF-16/FOXO transcription factor in neurons, but not that of NPR-1.Conversely, response to weak stimuli did not require the function of DAF-16/FOXO.These findings suggest that routine homeostatic stabilization of sleep may be distinct from homeostatic compensation following a strong disturbance.

View Article: PubMed Central - PubMed

Affiliation: Institute for Biophysical Dynamics, University of Chicago, Chicago, United States.

ABSTRACT
Biological homeostasis invokes modulatory responses aimed at stabilizing internal conditions. Using tunable photo- and mechano-stimulation, we identified two distinct categories of homeostatic responses during the sleep-like state of Caenorhabditis elegans (lethargus). In the presence of weak or no stimuli, extended motion caused a subsequent extension of quiescence. The neuropeptide Y receptor homolog, NPR-1, and an inhibitory neuropeptide known to activate it, FLP-18, were required for this process. In the presence of strong stimuli, the correlations between motion and quiescence were disrupted for several minutes but homeostasis manifested as an overall elevation of the time spent in quiescence. This response to strong stimuli required the function of the DAF-16/FOXO transcription factor in neurons, but not that of NPR-1. Conversely, response to weak stimuli did not require the function of DAF-16/FOXO. These findings suggest that routine homeostatic stabilization of sleep may be distinct from homeostatic compensation following a strong disturbance.

Show MeSH