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Mushroom body output neurons encode valence and guide memory-based action selection in Drosophila.

Aso Y, Sitaraman D, Ichinose T, Kaun KR, Vogt K, Belliart-Guérin G, Plaçais PY, Robie AA, Yamagata N, Schnaitmann C, Rowell WJ, Johnston RM, Ngo TT, Chen N, Korff W, Nitabach MN, Heberlein U, Preat T, Branson KM, Tanimoto H, Rubin GM - Elife (2014)

Bottom Line: The behavioral effects of MBON perturbation are combinatorial, suggesting that the MBON ensemble collectively represents valence.We propose that local, stimulus-specific dopaminergic modulation selectively alters the balance within the MBON network for those stimuli.Our results suggest that valence encoded by the MBON ensemble biases memory-based action selection.

View Article: PubMed Central - PubMed

Affiliation: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.

ABSTRACT
Animals discriminate stimuli, learn their predictive value and use this knowledge to modify their behavior. In Drosophila, the mushroom body (MB) plays a key role in these processes. Sensory stimuli are sparsely represented by ∼2000 Kenyon cells, which converge onto 34 output neurons (MBONs) of 21 types. We studied the role of MBONs in several associative learning tasks and in sleep regulation, revealing the extent to which information flow is segregated into distinct channels and suggesting possible roles for the multi-layered MBON network. We also show that optogenetic activation of MBONs can, depending on cell type, induce repulsion or attraction in flies. The behavioral effects of MBON perturbation are combinatorial, suggesting that the MBON ensemble collectively represents valence. We propose that local, stimulus-specific dopaminergic modulation selectively alters the balance within the MBON network for those stimuli. Our results suggest that valence encoded by the MBON ensemble biases memory-based action selection.

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Expression patterns of split-GAL4s with UAS-CsChrimson.(A–J) Anatomy of MBONs in additional split-GAL4 drivers. See legend to Figure 3A–F for explanation. Full confocal stacks of these images are available at www.janelia.org/split-gal4.DOI:http://dx.doi.org/10.7554/eLife.04580.008
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fig3s2: Expression patterns of split-GAL4s with UAS-CsChrimson.(A–J) Anatomy of MBONs in additional split-GAL4 drivers. See legend to Figure 3A–F for explanation. Full confocal stacks of these images are available at www.janelia.org/split-gal4.DOI:http://dx.doi.org/10.7554/eLife.04580.008


Mushroom body output neurons encode valence and guide memory-based action selection in Drosophila.

Aso Y, Sitaraman D, Ichinose T, Kaun KR, Vogt K, Belliart-Guérin G, Plaçais PY, Robie AA, Yamagata N, Schnaitmann C, Rowell WJ, Johnston RM, Ngo TT, Chen N, Korff W, Nitabach MN, Heberlein U, Preat T, Branson KM, Tanimoto H, Rubin GM - Elife (2014)

Expression patterns of split-GAL4s with UAS-CsChrimson.(A–J) Anatomy of MBONs in additional split-GAL4 drivers. See legend to Figure 3A–F for explanation. Full confocal stacks of these images are available at www.janelia.org/split-gal4.DOI:http://dx.doi.org/10.7554/eLife.04580.008
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4273436&req=5

fig3s2: Expression patterns of split-GAL4s with UAS-CsChrimson.(A–J) Anatomy of MBONs in additional split-GAL4 drivers. See legend to Figure 3A–F for explanation. Full confocal stacks of these images are available at www.janelia.org/split-gal4.DOI:http://dx.doi.org/10.7554/eLife.04580.008
Bottom Line: The behavioral effects of MBON perturbation are combinatorial, suggesting that the MBON ensemble collectively represents valence.We propose that local, stimulus-specific dopaminergic modulation selectively alters the balance within the MBON network for those stimuli.Our results suggest that valence encoded by the MBON ensemble biases memory-based action selection.

View Article: PubMed Central - PubMed

Affiliation: Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.

ABSTRACT
Animals discriminate stimuli, learn their predictive value and use this knowledge to modify their behavior. In Drosophila, the mushroom body (MB) plays a key role in these processes. Sensory stimuli are sparsely represented by ∼2000 Kenyon cells, which converge onto 34 output neurons (MBONs) of 21 types. We studied the role of MBONs in several associative learning tasks and in sleep regulation, revealing the extent to which information flow is segregated into distinct channels and suggesting possible roles for the multi-layered MBON network. We also show that optogenetic activation of MBONs can, depending on cell type, induce repulsion or attraction in flies. The behavioral effects of MBON perturbation are combinatorial, suggesting that the MBON ensemble collectively represents valence. We propose that local, stimulus-specific dopaminergic modulation selectively alters the balance within the MBON network for those stimuli. Our results suggest that valence encoded by the MBON ensemble biases memory-based action selection.

Show MeSH