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Single-shot compressed ultrafast photography at one hundred billion frames per second.

Gao L, Liang J, Li C, Wang LV - Nature (2014)

Bottom Line: Furthermore, akin to traditional photography, CUP is receive-only, and so does not need the specialized active illumination required by other single-shot ultrafast imagers.As a result, CUP can image a variety of luminescent--such as fluorescent or bioluminescent--objects.Given CUP's capability, we expect it to find widespread applications in both fundamental and applied sciences, including biomedical research.

View Article: PubMed Central - PubMed

Affiliation: Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St Louis, Campus Box 1097, One Brookings Drive, St Louis, Missouri 63130, USA.

ABSTRACT
The capture of transient scenes at high imaging speed has been long sought by photographers, with early examples being the well known recording in 1878 of a horse in motion and the 1887 photograph of a supersonic bullet. However, not until the late twentieth century were breakthroughs achieved in demonstrating ultrahigh-speed imaging (more than 10(5) frames per second). In particular, the introduction of electronic imaging sensors based on the charge-coupled device (CCD) or complementary metal-oxide-semiconductor (CMOS) technology revolutionized high-speed photography, enabling acquisition rates of up to 10(7) frames per second. Despite these sensors' widespread impact, further increasing frame rates using CCD or CMOS technology is fundamentally limited by their on-chip storage and electronic readout speed. Here we demonstrate a two-dimensional dynamic imaging technique, compressed ultrafast photography (CUP), which can capture non-repetitive time-evolving events at up to 10(11) frames per second. Compared with existing ultrafast imaging techniques, CUP has the prominent advantage of measuring an x-y-t (x, y, spatial coordinates; t, time) scene with a single camera snapshot, thereby allowing observation of transient events with temporal resolution as tens of picoseconds. Furthermore, akin to traditional photography, CUP is receive-only, and so does not need the specialized active illumination required by other single-shot ultrafast imagers. As a result, CUP can image a variety of luminescent--such as fluorescent or bioluminescent--objects. Using CUP, we visualize four fundamental physical phenomena with single laser shots only: laser pulse reflection and refraction, photon racing in two media, and faster-than-light propagation of non-information (that is, motion that appears faster than the speed of light but cannot convey information). Given CUP's capability, we expect it to find widespread applications in both fundamental and applied sciences, including biomedical research.

No MeSH data available.


Related in: MedlinePlus

Multicolour CUP. a. Custom-built spectral separation unit. b. Representative temporal frames of a pulsed-laser-pumped fluorescence emission process. The pulsed pump laser and fluorescence emission are pseudo-coloured based on their peak emission wavelengths. To explicitly indicate the spatiotemporal pattern of this event, the CUP-reconstructed frames are overlaid with a static background image captured by a monochromatic CCD camera. All temporal frames of this event are provided in Supplementary Video 6. c. Time-lapse pump laser and fluorescence emission intensities averaged within the dashed box in b. The temporal responses of pump laser excitation and fluorescence decay are fitted to a Gaussian function and an exponential function, respectively. The recovered fluorescence lifetime of Rhodamine 6G is 3.8 ns. d. Event function describing the pulsed laser fluorescence excitation. e. Event function describing the fluorescence emission. f. Measured temporal PSF, with a full width at half maximum of ~80 ps. Due to reconstruction artefacts, the PSF has a side lobe and a shoulder extending over a range of 280 ps. g. Simulated temporal responses of these two event functions after being convolved with the temporal PSF. The maxima of these two time-lapse signals are stretched by 200 ps. Scale bar, 10 mm.
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Figure 7: Multicolour CUP. a. Custom-built spectral separation unit. b. Representative temporal frames of a pulsed-laser-pumped fluorescence emission process. The pulsed pump laser and fluorescence emission are pseudo-coloured based on their peak emission wavelengths. To explicitly indicate the spatiotemporal pattern of this event, the CUP-reconstructed frames are overlaid with a static background image captured by a monochromatic CCD camera. All temporal frames of this event are provided in Supplementary Video 6. c. Time-lapse pump laser and fluorescence emission intensities averaged within the dashed box in b. The temporal responses of pump laser excitation and fluorescence decay are fitted to a Gaussian function and an exponential function, respectively. The recovered fluorescence lifetime of Rhodamine 6G is 3.8 ns. d. Event function describing the pulsed laser fluorescence excitation. e. Event function describing the fluorescence emission. f. Measured temporal PSF, with a full width at half maximum of ~80 ps. Due to reconstruction artefacts, the PSF has a side lobe and a shoulder extending over a range of 280 ps. g. Simulated temporal responses of these two event functions after being convolved with the temporal PSF. The maxima of these two time-lapse signals are stretched by 200 ps. Scale bar, 10 mm.


Single-shot compressed ultrafast photography at one hundred billion frames per second.

Gao L, Liang J, Li C, Wang LV - Nature (2014)

Multicolour CUP. a. Custom-built spectral separation unit. b. Representative temporal frames of a pulsed-laser-pumped fluorescence emission process. The pulsed pump laser and fluorescence emission are pseudo-coloured based on their peak emission wavelengths. To explicitly indicate the spatiotemporal pattern of this event, the CUP-reconstructed frames are overlaid with a static background image captured by a monochromatic CCD camera. All temporal frames of this event are provided in Supplementary Video 6. c. Time-lapse pump laser and fluorescence emission intensities averaged within the dashed box in b. The temporal responses of pump laser excitation and fluorescence decay are fitted to a Gaussian function and an exponential function, respectively. The recovered fluorescence lifetime of Rhodamine 6G is 3.8 ns. d. Event function describing the pulsed laser fluorescence excitation. e. Event function describing the fluorescence emission. f. Measured temporal PSF, with a full width at half maximum of ~80 ps. Due to reconstruction artefacts, the PSF has a side lobe and a shoulder extending over a range of 280 ps. g. Simulated temporal responses of these two event functions after being convolved with the temporal PSF. The maxima of these two time-lapse signals are stretched by 200 ps. Scale bar, 10 mm.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4270006&req=5

Figure 7: Multicolour CUP. a. Custom-built spectral separation unit. b. Representative temporal frames of a pulsed-laser-pumped fluorescence emission process. The pulsed pump laser and fluorescence emission are pseudo-coloured based on their peak emission wavelengths. To explicitly indicate the spatiotemporal pattern of this event, the CUP-reconstructed frames are overlaid with a static background image captured by a monochromatic CCD camera. All temporal frames of this event are provided in Supplementary Video 6. c. Time-lapse pump laser and fluorescence emission intensities averaged within the dashed box in b. The temporal responses of pump laser excitation and fluorescence decay are fitted to a Gaussian function and an exponential function, respectively. The recovered fluorescence lifetime of Rhodamine 6G is 3.8 ns. d. Event function describing the pulsed laser fluorescence excitation. e. Event function describing the fluorescence emission. f. Measured temporal PSF, with a full width at half maximum of ~80 ps. Due to reconstruction artefacts, the PSF has a side lobe and a shoulder extending over a range of 280 ps. g. Simulated temporal responses of these two event functions after being convolved with the temporal PSF. The maxima of these two time-lapse signals are stretched by 200 ps. Scale bar, 10 mm.
Bottom Line: Furthermore, akin to traditional photography, CUP is receive-only, and so does not need the specialized active illumination required by other single-shot ultrafast imagers.As a result, CUP can image a variety of luminescent--such as fluorescent or bioluminescent--objects.Given CUP's capability, we expect it to find widespread applications in both fundamental and applied sciences, including biomedical research.

View Article: PubMed Central - PubMed

Affiliation: Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St Louis, Campus Box 1097, One Brookings Drive, St Louis, Missouri 63130, USA.

ABSTRACT
The capture of transient scenes at high imaging speed has been long sought by photographers, with early examples being the well known recording in 1878 of a horse in motion and the 1887 photograph of a supersonic bullet. However, not until the late twentieth century were breakthroughs achieved in demonstrating ultrahigh-speed imaging (more than 10(5) frames per second). In particular, the introduction of electronic imaging sensors based on the charge-coupled device (CCD) or complementary metal-oxide-semiconductor (CMOS) technology revolutionized high-speed photography, enabling acquisition rates of up to 10(7) frames per second. Despite these sensors' widespread impact, further increasing frame rates using CCD or CMOS technology is fundamentally limited by their on-chip storage and electronic readout speed. Here we demonstrate a two-dimensional dynamic imaging technique, compressed ultrafast photography (CUP), which can capture non-repetitive time-evolving events at up to 10(11) frames per second. Compared with existing ultrafast imaging techniques, CUP has the prominent advantage of measuring an x-y-t (x, y, spatial coordinates; t, time) scene with a single camera snapshot, thereby allowing observation of transient events with temporal resolution as tens of picoseconds. Furthermore, akin to traditional photography, CUP is receive-only, and so does not need the specialized active illumination required by other single-shot ultrafast imagers. As a result, CUP can image a variety of luminescent--such as fluorescent or bioluminescent--objects. Using CUP, we visualize four fundamental physical phenomena with single laser shots only: laser pulse reflection and refraction, photon racing in two media, and faster-than-light propagation of non-information (that is, motion that appears faster than the speed of light but cannot convey information). Given CUP's capability, we expect it to find widespread applications in both fundamental and applied sciences, including biomedical research.

No MeSH data available.


Related in: MedlinePlus