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Reducing X-Ray Induced Oxidative Damages in Fibroblasts with Graphene Oxide.

Qiao Y, Zhang P, Wang C, Ma L, Su M - Nanomaterials (Basel) (2014)

Bottom Line: A major issue of X-ray radiation therapy is that normal cells can be damaged, limiting the amount of X-rays that can be safely delivered to a tumor.A variety of techniques such as cytotoxicity, genotoxicity, oxidative assay, apoptosis, γ-H2AX expression, and micro-nucleus assay have been used to assess the protective effect of GO in cultured fibroblast cells.Thus, low concentration GO can be used as an effective radio-protective agent in occupational and therapeutic settings.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biomedical Engineering, Worcester Polytechnic Institute, Worcester, MA 01609, USA.

ABSTRACT

A major issue of X-ray radiation therapy is that normal cells can be damaged, limiting the amount of X-rays that can be safely delivered to a tumor. This paper describes a new method based on graphene oxide (GO) to protect normal cells from oxidative damage by removing free radicals generated by X-ray radiation using grapheme oxide (GO). A variety of techniques such as cytotoxicity, genotoxicity, oxidative assay, apoptosis, γ-H2AX expression, and micro-nucleus assay have been used to assess the protective effect of GO in cultured fibroblast cells. It is found that although GO at higher concentration (100 and 500 μg/mL) can cause cell death and DNA damage, it can effectively remove oxygen free radicals at a lower concentration of 10 μg/mL. The level of DNA damage and cell death is reduced by 48%, and 39%, respectively. Thus, low concentration GO can be used as an effective radio-protective agent in occupational and therapeutic settings.

No MeSH data available.


Related in: MedlinePlus

Flow cytometry evaluation of cell apoptosis: cells (A); cells treated with GO (B); cells exposed to 1.25 Gy X-ray (C); and cells treated with 10 μg/mL GO and then exposed to 1.25 Gy X-ray (D).
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Figure 5: Flow cytometry evaluation of cell apoptosis: cells (A); cells treated with GO (B); cells exposed to 1.25 Gy X-ray (C); and cells treated with 10 μg/mL GO and then exposed to 1.25 Gy X-ray (D).

Mentions: Annexin V-FITC apoptosis detection kit is used to study X-ray irradiation, GO induced apoptosis, and necrosis of fibroblasts [34]. Four samples are studied: (1) cells; (2) cells treated with 10 μg/mL; (3) cells exposed to 1.25 Gy X-ray and (4) cells treated with 10 μg/mL GO and then exposed to 1.25 Gy X-ray. The percentages of apoptosis are 0% and 0.3% in sample 1 (Figure 5A) and sample 2 (Figure 5B), respectively. Figure 5C shows that 55.1% of cells exposed to X-ray undergo apoptosis. Figure 5D shows that 16.1% of cells treated with GO and then exposed to X-ray undergo apoptosis. The results show that no significant change in apoptosis is observed in GO treated cells. After GO treatment, X-ray radiation can only causes a small amount of cells undergo apoptosis.


Reducing X-Ray Induced Oxidative Damages in Fibroblasts with Graphene Oxide.

Qiao Y, Zhang P, Wang C, Ma L, Su M - Nanomaterials (Basel) (2014)

Flow cytometry evaluation of cell apoptosis: cells (A); cells treated with GO (B); cells exposed to 1.25 Gy X-ray (C); and cells treated with 10 μg/mL GO and then exposed to 1.25 Gy X-ray (D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4269382&req=5

Figure 5: Flow cytometry evaluation of cell apoptosis: cells (A); cells treated with GO (B); cells exposed to 1.25 Gy X-ray (C); and cells treated with 10 μg/mL GO and then exposed to 1.25 Gy X-ray (D).
Mentions: Annexin V-FITC apoptosis detection kit is used to study X-ray irradiation, GO induced apoptosis, and necrosis of fibroblasts [34]. Four samples are studied: (1) cells; (2) cells treated with 10 μg/mL; (3) cells exposed to 1.25 Gy X-ray and (4) cells treated with 10 μg/mL GO and then exposed to 1.25 Gy X-ray. The percentages of apoptosis are 0% and 0.3% in sample 1 (Figure 5A) and sample 2 (Figure 5B), respectively. Figure 5C shows that 55.1% of cells exposed to X-ray undergo apoptosis. Figure 5D shows that 16.1% of cells treated with GO and then exposed to X-ray undergo apoptosis. The results show that no significant change in apoptosis is observed in GO treated cells. After GO treatment, X-ray radiation can only causes a small amount of cells undergo apoptosis.

Bottom Line: A major issue of X-ray radiation therapy is that normal cells can be damaged, limiting the amount of X-rays that can be safely delivered to a tumor.A variety of techniques such as cytotoxicity, genotoxicity, oxidative assay, apoptosis, γ-H2AX expression, and micro-nucleus assay have been used to assess the protective effect of GO in cultured fibroblast cells.Thus, low concentration GO can be used as an effective radio-protective agent in occupational and therapeutic settings.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biomedical Engineering, Worcester Polytechnic Institute, Worcester, MA 01609, USA.

ABSTRACT

A major issue of X-ray radiation therapy is that normal cells can be damaged, limiting the amount of X-rays that can be safely delivered to a tumor. This paper describes a new method based on graphene oxide (GO) to protect normal cells from oxidative damage by removing free radicals generated by X-ray radiation using grapheme oxide (GO). A variety of techniques such as cytotoxicity, genotoxicity, oxidative assay, apoptosis, γ-H2AX expression, and micro-nucleus assay have been used to assess the protective effect of GO in cultured fibroblast cells. It is found that although GO at higher concentration (100 and 500 μg/mL) can cause cell death and DNA damage, it can effectively remove oxygen free radicals at a lower concentration of 10 μg/mL. The level of DNA damage and cell death is reduced by 48%, and 39%, respectively. Thus, low concentration GO can be used as an effective radio-protective agent in occupational and therapeutic settings.

No MeSH data available.


Related in: MedlinePlus