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Reducing X-Ray Induced Oxidative Damages in Fibroblasts with Graphene Oxide.

Qiao Y, Zhang P, Wang C, Ma L, Su M - Nanomaterials (Basel) (2014)

Bottom Line: A major issue of X-ray radiation therapy is that normal cells can be damaged, limiting the amount of X-rays that can be safely delivered to a tumor.A variety of techniques such as cytotoxicity, genotoxicity, oxidative assay, apoptosis, γ-H2AX expression, and micro-nucleus assay have been used to assess the protective effect of GO in cultured fibroblast cells.Thus, low concentration GO can be used as an effective radio-protective agent in occupational and therapeutic settings.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biomedical Engineering, Worcester Polytechnic Institute, Worcester, MA 01609, USA.

ABSTRACT

A major issue of X-ray radiation therapy is that normal cells can be damaged, limiting the amount of X-rays that can be safely delivered to a tumor. This paper describes a new method based on graphene oxide (GO) to protect normal cells from oxidative damage by removing free radicals generated by X-ray radiation using grapheme oxide (GO). A variety of techniques such as cytotoxicity, genotoxicity, oxidative assay, apoptosis, γ-H2AX expression, and micro-nucleus assay have been used to assess the protective effect of GO in cultured fibroblast cells. It is found that although GO at higher concentration (100 and 500 μg/mL) can cause cell death and DNA damage, it can effectively remove oxygen free radicals at a lower concentration of 10 μg/mL. The level of DNA damage and cell death is reduced by 48%, and 39%, respectively. Thus, low concentration GO can be used as an effective radio-protective agent in occupational and therapeutic settings.

No MeSH data available.


Related in: MedlinePlus

Optical images of cells (A) and cells treated with graphene oxide (GO) for 24 h (B); cytotoxicity of cells treated with different concentrations of GO (C); and exposed to different dose of X-ray (D). “*” (p < 0.05) and “**” (p < 0.01) represent significant difference and extra significant difference, respectively.
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Figure 1: Optical images of cells (A) and cells treated with graphene oxide (GO) for 24 h (B); cytotoxicity of cells treated with different concentrations of GO (C); and exposed to different dose of X-ray (D). “*” (p < 0.05) and “**” (p < 0.01) represent significant difference and extra significant difference, respectively.

Mentions: Figure 1A,B shows optical images of fibroblasts cultures that were treated with 0 and 10 μg/mL GO for 24 h at 37 °C. Compared to 1A, the morphology of cells in 1B does not change, though many GO particles are attached on the cell surface. After replacing GO-containing medium, cells are rinsed by PBS for three times and exposed to X-ray radiation. MTT assay results show that viability of GO-treated cells decrease as GO concentration increases from 1 to 500 μg/mL (Figure 1C). At low concentration (1 and 10 μg/mL), GO does not have significant toxicity [28,29]. At high concentrations, 100 μg/mL and 500 μg/mL, cell viability decreases, where 30% and 48% of cells are killed respectively. In order to test cytotoxicity induced by X-ray radiation, fibroblasts are exposed to different doses of X-ray radiation. Figure 1D shows cell viability decreases as X-ray dose increases from 0 to 2.5 Gy. When the dose is in the range from 0 to 0.75 Gy, cell viability shows significant difference decreases (p < 0.05); while when the dose is higher than 1.25 Gy, cell viability shows extra significant difference decreases (p < 0.01). As low dose and low GO concentration can decrease side effects, 10 μg/mL GO and 1.25 Gy X-ray have been chosen in the following experiment.


Reducing X-Ray Induced Oxidative Damages in Fibroblasts with Graphene Oxide.

Qiao Y, Zhang P, Wang C, Ma L, Su M - Nanomaterials (Basel) (2014)

Optical images of cells (A) and cells treated with graphene oxide (GO) for 24 h (B); cytotoxicity of cells treated with different concentrations of GO (C); and exposed to different dose of X-ray (D). “*” (p < 0.05) and “**” (p < 0.01) represent significant difference and extra significant difference, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4269382&req=5

Figure 1: Optical images of cells (A) and cells treated with graphene oxide (GO) for 24 h (B); cytotoxicity of cells treated with different concentrations of GO (C); and exposed to different dose of X-ray (D). “*” (p < 0.05) and “**” (p < 0.01) represent significant difference and extra significant difference, respectively.
Mentions: Figure 1A,B shows optical images of fibroblasts cultures that were treated with 0 and 10 μg/mL GO for 24 h at 37 °C. Compared to 1A, the morphology of cells in 1B does not change, though many GO particles are attached on the cell surface. After replacing GO-containing medium, cells are rinsed by PBS for three times and exposed to X-ray radiation. MTT assay results show that viability of GO-treated cells decrease as GO concentration increases from 1 to 500 μg/mL (Figure 1C). At low concentration (1 and 10 μg/mL), GO does not have significant toxicity [28,29]. At high concentrations, 100 μg/mL and 500 μg/mL, cell viability decreases, where 30% and 48% of cells are killed respectively. In order to test cytotoxicity induced by X-ray radiation, fibroblasts are exposed to different doses of X-ray radiation. Figure 1D shows cell viability decreases as X-ray dose increases from 0 to 2.5 Gy. When the dose is in the range from 0 to 0.75 Gy, cell viability shows significant difference decreases (p < 0.05); while when the dose is higher than 1.25 Gy, cell viability shows extra significant difference decreases (p < 0.01). As low dose and low GO concentration can decrease side effects, 10 μg/mL GO and 1.25 Gy X-ray have been chosen in the following experiment.

Bottom Line: A major issue of X-ray radiation therapy is that normal cells can be damaged, limiting the amount of X-rays that can be safely delivered to a tumor.A variety of techniques such as cytotoxicity, genotoxicity, oxidative assay, apoptosis, γ-H2AX expression, and micro-nucleus assay have been used to assess the protective effect of GO in cultured fibroblast cells.Thus, low concentration GO can be used as an effective radio-protective agent in occupational and therapeutic settings.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biomedical Engineering, Worcester Polytechnic Institute, Worcester, MA 01609, USA.

ABSTRACT

A major issue of X-ray radiation therapy is that normal cells can be damaged, limiting the amount of X-rays that can be safely delivered to a tumor. This paper describes a new method based on graphene oxide (GO) to protect normal cells from oxidative damage by removing free radicals generated by X-ray radiation using grapheme oxide (GO). A variety of techniques such as cytotoxicity, genotoxicity, oxidative assay, apoptosis, γ-H2AX expression, and micro-nucleus assay have been used to assess the protective effect of GO in cultured fibroblast cells. It is found that although GO at higher concentration (100 and 500 μg/mL) can cause cell death and DNA damage, it can effectively remove oxygen free radicals at a lower concentration of 10 μg/mL. The level of DNA damage and cell death is reduced by 48%, and 39%, respectively. Thus, low concentration GO can be used as an effective radio-protective agent in occupational and therapeutic settings.

No MeSH data available.


Related in: MedlinePlus