Limits...
Effects of acupuncture knife on inflammatory factors and pain in third lumbar vertebrae transverse process syndrome model rats.

Yu JN, Guo CQ, Hu B, Liu NG, Sun HM, Xu H, Wu HX, Guo Y, Liang CX, Chen ZX, Li XH - Evid Based Complement Alternat Med (2014)

Bottom Line: The aim of this paper was to explore the long-term effects and pain relief mechanism of acupuncture knife on third lumbar vertebrae (L3) transverse process syndrome.The tail flick test was used to observe pain threshold.AK pretreatment could alleviate pain through modulating inflammatory response.

View Article: PubMed Central - PubMed

Affiliation: School of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, No. 11 North 3rd Ring East Road, Chaoyang District, Beijing 100029, China.

ABSTRACT
The aim of this paper was to explore the long-term effects and pain relief mechanism of acupuncture knife on third lumbar vertebrae (L3) transverse process syndrome. Forty SD rats were randomized into control, model, electroacupuncture (EA), and acupuncture knife (AK) group. Except control rats, other rats were subjected to an operation to emulate L3 transverse process syndrome. Fourteen days after the operation, EA and AK rats were given electroacupuncture and acupuncture knife treatments, respectively. Fifty-six days after the operation, enzyme-linked immunosorbent assay was used to measure substance P (SP), 5-hydroxytryptamine (5-HT), interleukin-1β (IL-1β), interleukin-10 (IL-10), tumor necrosis factor-α (TNF-α), and transforming growth factor-β (TGF-β) in peripheral blood. The tail flick test was used to observe pain threshold. We found that rats with the simulation operation had significantly higher levels of SP, 5-HT, IL-1, IL-10, TNF-α, and TGF-β, while the AK rats had lower levels. In addition, the pain threshold of AK rats was similar to that of control rats. AK pretreatment could alleviate pain through modulating inflammatory response.

No MeSH data available.


Related in: MedlinePlus

Representative ultrastructure changes of skeletal muscles after treatments in the following groups (n = 10 per group): (a) control, (b) model, (c) EA, and (d) AK.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4269310&req=5

fig1: Representative ultrastructure changes of skeletal muscles after treatments in the following groups (n = 10 per group): (a) control, (b) model, (c) EA, and (d) AK.

Mentions: In the control group, the structure of sarcomeres was well aligned, with orderly thick and thin filaments. A-bank, I-bank, H-bank, M-line, and Z-line were clear and regular. Around the junction of the A-bank and I-bank, there was a sarcoplasmic reticulum with obvious triads. The structure of mitochondria was clear with normal cristae. The sarcoplasmic reticulum distribution was regular with integrated muscle membrane (Figure 1(a)). In the model group, there was excessive empty space between the myofibrils. The structure of myofibrils and sarcomeres was disordered. A-bank, I-bank, H-bank, M-line, and Z-line were irregular or not present. Some thick or thin filaments were fractured. Swelling of mitochondria was observed. The number of irregularly arranged collagenous fibers was increased (Figure 1(b)). In the EA group, the empty space between the myofibrils was smaller than that in the model group. The number of fractured filaments was lower than that in the model group. Collagenous fibers were evident (Figure 1(c)). In the AK group, the empty space between the myofibrils was smaller than that in the model group. A small number of filaments were fractured. The structure of mitochondria was almost normal. Collagenous fibers were observed, but arrangement was orderly (Figure 1(d)).


Effects of acupuncture knife on inflammatory factors and pain in third lumbar vertebrae transverse process syndrome model rats.

Yu JN, Guo CQ, Hu B, Liu NG, Sun HM, Xu H, Wu HX, Guo Y, Liang CX, Chen ZX, Li XH - Evid Based Complement Alternat Med (2014)

Representative ultrastructure changes of skeletal muscles after treatments in the following groups (n = 10 per group): (a) control, (b) model, (c) EA, and (d) AK.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4269310&req=5

fig1: Representative ultrastructure changes of skeletal muscles after treatments in the following groups (n = 10 per group): (a) control, (b) model, (c) EA, and (d) AK.
Mentions: In the control group, the structure of sarcomeres was well aligned, with orderly thick and thin filaments. A-bank, I-bank, H-bank, M-line, and Z-line were clear and regular. Around the junction of the A-bank and I-bank, there was a sarcoplasmic reticulum with obvious triads. The structure of mitochondria was clear with normal cristae. The sarcoplasmic reticulum distribution was regular with integrated muscle membrane (Figure 1(a)). In the model group, there was excessive empty space between the myofibrils. The structure of myofibrils and sarcomeres was disordered. A-bank, I-bank, H-bank, M-line, and Z-line were irregular or not present. Some thick or thin filaments were fractured. Swelling of mitochondria was observed. The number of irregularly arranged collagenous fibers was increased (Figure 1(b)). In the EA group, the empty space between the myofibrils was smaller than that in the model group. The number of fractured filaments was lower than that in the model group. Collagenous fibers were evident (Figure 1(c)). In the AK group, the empty space between the myofibrils was smaller than that in the model group. A small number of filaments were fractured. The structure of mitochondria was almost normal. Collagenous fibers were observed, but arrangement was orderly (Figure 1(d)).

Bottom Line: The aim of this paper was to explore the long-term effects and pain relief mechanism of acupuncture knife on third lumbar vertebrae (L3) transverse process syndrome.The tail flick test was used to observe pain threshold.AK pretreatment could alleviate pain through modulating inflammatory response.

View Article: PubMed Central - PubMed

Affiliation: School of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, No. 11 North 3rd Ring East Road, Chaoyang District, Beijing 100029, China.

ABSTRACT
The aim of this paper was to explore the long-term effects and pain relief mechanism of acupuncture knife on third lumbar vertebrae (L3) transverse process syndrome. Forty SD rats were randomized into control, model, electroacupuncture (EA), and acupuncture knife (AK) group. Except control rats, other rats were subjected to an operation to emulate L3 transverse process syndrome. Fourteen days after the operation, EA and AK rats were given electroacupuncture and acupuncture knife treatments, respectively. Fifty-six days after the operation, enzyme-linked immunosorbent assay was used to measure substance P (SP), 5-hydroxytryptamine (5-HT), interleukin-1β (IL-1β), interleukin-10 (IL-10), tumor necrosis factor-α (TNF-α), and transforming growth factor-β (TGF-β) in peripheral blood. The tail flick test was used to observe pain threshold. We found that rats with the simulation operation had significantly higher levels of SP, 5-HT, IL-1, IL-10, TNF-α, and TGF-β, while the AK rats had lower levels. In addition, the pain threshold of AK rats was similar to that of control rats. AK pretreatment could alleviate pain through modulating inflammatory response.

No MeSH data available.


Related in: MedlinePlus