Limits...
Characteristic Fingerprint Based on Low Polar Constituents for Discrimination of Wolfiporia extensa according to Geographical Origin Using UV Spectroscopy and Chemometrics Methods.

Li Y, Zhang J, Zhao Y, Li Z, Li T, Wang Y - J Anal Methods Chem (2014)

Bottom Line: The results showed that W. extensa samples were well classified according to their geographical origins.The proposed method can fully utilize diversified fingerprint characteristics of sclerotium of W. extensa and requires low-cost equipment and short-time analysis in comparison with other techniques.Meanwhile, this simple and efficient method may serve as a basis for the authentication of other medicinal fungi.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China ; College of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China.

ABSTRACT
The fungus species Wolfiporia extensa has a long history of medicinal usage and has also been commercially used to formulate nutraceuticals and functional foods in certain Asian countries. In the present study, a practical and promising method has been developed to discriminate the dried sclerotium of W. extensa collected from different geographical sites based on UV spectroscopy together with chemometrics methods. Characteristic fingerprint of low polar constituents of sample extracts that originated from chloroform has been obtained in the interval 250-400 nm. Chemometric pattern recognition methods such as partial least squares discriminant analysis (PLS-DA) and hierarchical cluster analysis (HCA) were applied to enhance the authenticity of discrimination of the specimens. The results showed that W. extensa samples were well classified according to their geographical origins. The proposed method can fully utilize diversified fingerprint characteristics of sclerotium of W. extensa and requires low-cost equipment and short-time analysis in comparison with other techniques. Meanwhile, this simple and efficient method may serve as a basis for the authentication of other medicinal fungi.

No MeSH data available.


Three-dimensional wireframe plot of UV spectra of W. extensa sclerotium specimens.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4269309&req=5

fig3: Three-dimensional wireframe plot of UV spectra of W. extensa sclerotium specimens.

Mentions: The UV absorption bands of the presented samples are usually associated with the presence of different chromophores exemplified in conjugated systems as well as other UV-absorbing systems [49]. The UV spectrum of each of the studied W. extensa sclerotium samples was recorded in the region between 190 and 400 nm. On account of the detection range of the UV-visible spectrometer, we chose the wavelengths of absorption peaks arranged from 250 to 400 nm for the sake of avoiding the spectral noise. The UV spectra for all the samples are presented in Figures 3 and 4.


Characteristic Fingerprint Based on Low Polar Constituents for Discrimination of Wolfiporia extensa according to Geographical Origin Using UV Spectroscopy and Chemometrics Methods.

Li Y, Zhang J, Zhao Y, Li Z, Li T, Wang Y - J Anal Methods Chem (2014)

Three-dimensional wireframe plot of UV spectra of W. extensa sclerotium specimens.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4269309&req=5

fig3: Three-dimensional wireframe plot of UV spectra of W. extensa sclerotium specimens.
Mentions: The UV absorption bands of the presented samples are usually associated with the presence of different chromophores exemplified in conjugated systems as well as other UV-absorbing systems [49]. The UV spectrum of each of the studied W. extensa sclerotium samples was recorded in the region between 190 and 400 nm. On account of the detection range of the UV-visible spectrometer, we chose the wavelengths of absorption peaks arranged from 250 to 400 nm for the sake of avoiding the spectral noise. The UV spectra for all the samples are presented in Figures 3 and 4.

Bottom Line: The results showed that W. extensa samples were well classified according to their geographical origins.The proposed method can fully utilize diversified fingerprint characteristics of sclerotium of W. extensa and requires low-cost equipment and short-time analysis in comparison with other techniques.Meanwhile, this simple and efficient method may serve as a basis for the authentication of other medicinal fungi.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China ; College of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China.

ABSTRACT
The fungus species Wolfiporia extensa has a long history of medicinal usage and has also been commercially used to formulate nutraceuticals and functional foods in certain Asian countries. In the present study, a practical and promising method has been developed to discriminate the dried sclerotium of W. extensa collected from different geographical sites based on UV spectroscopy together with chemometrics methods. Characteristic fingerprint of low polar constituents of sample extracts that originated from chloroform has been obtained in the interval 250-400 nm. Chemometric pattern recognition methods such as partial least squares discriminant analysis (PLS-DA) and hierarchical cluster analysis (HCA) were applied to enhance the authenticity of discrimination of the specimens. The results showed that W. extensa samples were well classified according to their geographical origins. The proposed method can fully utilize diversified fingerprint characteristics of sclerotium of W. extensa and requires low-cost equipment and short-time analysis in comparison with other techniques. Meanwhile, this simple and efficient method may serve as a basis for the authentication of other medicinal fungi.

No MeSH data available.