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Glutamic acid decarboxylase 67 expression by a distinct population of mouse vestibular supporting cells.

Tavazzani E, Tritto S, Spaiardi P, Botta L, Manca M, Prigioni I, Masetto S, Russo G - Front Cell Neurosci (2014)

Bottom Line: While no evidence for GAD65 expression was found, GAD67 was detected in a distinct population of peripherally-located supporting cells, but not in hair cells or in centrally-located supporting cells.GABA, on the other hand, was found in all supporting cells.The present result indicate that only a discrete population of supporting cells use GAD67 to synthesize GABA.

View Article: PubMed Central - PubMed

Affiliation: Department of Brain and Behavioral Sciences, University of Pavia Pavia, Italy.

ABSTRACT
The function of the enzyme glutamate decarboxylase (GAD) is to convert glutamate in γ-aminobutyric acid (GABA). Glutamate decarboxylase exists as two major isoforms, termed GAD65 and GAD67, that are usually expressed in GABA-containing neurons in the central nervous system. GAD65 has been proposed to be associated with GABA exocytosis whereas GAD67 with GABA metabolism. In the present immunofluorescence study, we have investigated the presence of the two GAD isoforms in the semicircular canal cristae of wild type and GAD67-GFP knock-in mice. While no evidence for GAD65 expression was found, GAD67 was detected in a distinct population of peripherally-located supporting cells, but not in hair cells or in centrally-located supporting cells. GABA, on the other hand, was found in all supporting cells. The present result indicate that only a discrete population of supporting cells use GAD67 to synthesize GABA. This is the first report of a marker that allows to distinguish two populations of supporting cells in the vestibular epithelium. On the other hand, the lack of GABA and GAD enzymes in hair cells excludes its involvement in afferent transmission.

No MeSH data available.


Related in: MedlinePlus

Calbindin expression in the mouse horizontal crista. (A) Schematic representation showing the plane of the section. (B) Photomicrograph of a longitudinal confocal section showing the cell nuclei (blue; DAPI), GAD67 (green; GFP), and calbindin (red; antibody) expression—merged image. Note that most calbindin immunolabeling is concentrated in the central zone, where it marks several calyx endings.
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Figure 6: Calbindin expression in the mouse horizontal crista. (A) Schematic representation showing the plane of the section. (B) Photomicrograph of a longitudinal confocal section showing the cell nuclei (blue; DAPI), GAD67 (green; GFP), and calbindin (red; antibody) expression—merged image. Note that most calbindin immunolabeling is concentrated in the central zone, where it marks several calyx endings.

Mentions: In some experiments, an antibody for calbindin was also used to show the calyx nerve terminals (Lysakowski et al., 2011). Figure 6 shows such an example in a horizontal crista; note that calbindin antibody (red) clearly stained several afferent calyces, mostly located in the central zone, where GAD67 was conversely absent.


Glutamic acid decarboxylase 67 expression by a distinct population of mouse vestibular supporting cells.

Tavazzani E, Tritto S, Spaiardi P, Botta L, Manca M, Prigioni I, Masetto S, Russo G - Front Cell Neurosci (2014)

Calbindin expression in the mouse horizontal crista. (A) Schematic representation showing the plane of the section. (B) Photomicrograph of a longitudinal confocal section showing the cell nuclei (blue; DAPI), GAD67 (green; GFP), and calbindin (red; antibody) expression—merged image. Note that most calbindin immunolabeling is concentrated in the central zone, where it marks several calyx endings.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4269132&req=5

Figure 6: Calbindin expression in the mouse horizontal crista. (A) Schematic representation showing the plane of the section. (B) Photomicrograph of a longitudinal confocal section showing the cell nuclei (blue; DAPI), GAD67 (green; GFP), and calbindin (red; antibody) expression—merged image. Note that most calbindin immunolabeling is concentrated in the central zone, where it marks several calyx endings.
Mentions: In some experiments, an antibody for calbindin was also used to show the calyx nerve terminals (Lysakowski et al., 2011). Figure 6 shows such an example in a horizontal crista; note that calbindin antibody (red) clearly stained several afferent calyces, mostly located in the central zone, where GAD67 was conversely absent.

Bottom Line: While no evidence for GAD65 expression was found, GAD67 was detected in a distinct population of peripherally-located supporting cells, but not in hair cells or in centrally-located supporting cells.GABA, on the other hand, was found in all supporting cells.The present result indicate that only a discrete population of supporting cells use GAD67 to synthesize GABA.

View Article: PubMed Central - PubMed

Affiliation: Department of Brain and Behavioral Sciences, University of Pavia Pavia, Italy.

ABSTRACT
The function of the enzyme glutamate decarboxylase (GAD) is to convert glutamate in γ-aminobutyric acid (GABA). Glutamate decarboxylase exists as two major isoforms, termed GAD65 and GAD67, that are usually expressed in GABA-containing neurons in the central nervous system. GAD65 has been proposed to be associated with GABA exocytosis whereas GAD67 with GABA metabolism. In the present immunofluorescence study, we have investigated the presence of the two GAD isoforms in the semicircular canal cristae of wild type and GAD67-GFP knock-in mice. While no evidence for GAD65 expression was found, GAD67 was detected in a distinct population of peripherally-located supporting cells, but not in hair cells or in centrally-located supporting cells. GABA, on the other hand, was found in all supporting cells. The present result indicate that only a discrete population of supporting cells use GAD67 to synthesize GABA. This is the first report of a marker that allows to distinguish two populations of supporting cells in the vestibular epithelium. On the other hand, the lack of GABA and GAD enzymes in hair cells excludes its involvement in afferent transmission.

No MeSH data available.


Related in: MedlinePlus