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Long distance movement of an Arabidopsis Translationally Controlled Tumor Protein (AtTCTP2) mRNA and protein in tobacco.

Toscano-Morales R, Xoconostle-Cázares B, Martínez-Navarro AC, Ruiz-Medrano R - Front Plant Sci (2014)

Bottom Line: The results indicate that both AtTCTP2 mRNA and protein are capable of moving long distance in both directions (stock-scion and scion-stock) with a tendency for movement from source to sink tissue (stock to scion).In addition, the protein localization pattern in transgenic aerial and primary roots was basically the same, indicating specific nuclear destination in roots, but also in leaves.These findings provide an approach to understand the role of long-distance movement in the function of plant TCTPs, supporting the notion that some of these act in a non-cell autonomous manner, as the human counterpart, the Histamine Releasing Factor (HRF).

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology and Bioengineering, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional Mexico, Mexico.

ABSTRACT
Translationally Controlled Tumor Protein (TCTP) is an almost ubiquitous protein found in eukaryotes, fundamental for the regulation of development and general growth. The multiple functions of TCTP have been inferred from its involvement in several cell pathways, but the specific function of TCTP is still not known in detail. On the other hand, TCTP seems to respond to a plethora of external signals, and appears to be regulated at the transcriptional and/or translational levels by mechanisms yet to be determined. In the present work, we analyzed the capacity of AtTCTP2 gene products (mRNA and protein) to translocate long distance through tobacco heterografts (transgenic/WT and WT/transgenic). The results indicate that both AtTCTP2 mRNA and protein are capable of moving long distance in both directions (stock-scion and scion-stock) with a tendency for movement from source to sink tissue (stock to scion). Interestingly, aerial roots emerged only in heterografts where the protein was detected in both stock and scion, suggesting a correlation between the presence of AtTCTP2 and aerial root appearance. More detailed analysis showed that these aerial roots harbored the transgene and expressed both transcript and protein. In addition, the protein localization pattern in transgenic aerial and primary roots was basically the same, indicating specific nuclear destination in roots, but also in leaves. These findings provide an approach to understand the role of long-distance movement in the function of plant TCTPs, supporting the notion that some of these act in a non-cell autonomous manner, as the human counterpart, the Histamine Releasing Factor (HRF).

No MeSH data available.


Related in: MedlinePlus

Emergence of adventitious roots associated to long-distance movement of AtTCTP2. Representative images were taken previous tissue collection for RT-PCR and confocal analysis (30 days). (A) WT/AtTCTP2 grafts presented aerial roots in all samples, which is consistent with the protein detection in the same graft samples (see Table 1). (B: left and middle) AtTCTP2/WT grafts produced aerial roots in four out of seven trials, correlating specifically to the presence of AtTCTP2-GFP protein in the same graft samples (see Table 1 and Figure 2). (B: right) Hence, in the samples where no aerial roots were present no protein was observed. (C) The latter is consistent to the WT/WT graft controls.
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Figure 5: Emergence of adventitious roots associated to long-distance movement of AtTCTP2. Representative images were taken previous tissue collection for RT-PCR and confocal analysis (30 days). (A) WT/AtTCTP2 grafts presented aerial roots in all samples, which is consistent with the protein detection in the same graft samples (see Table 1). (B: left and middle) AtTCTP2/WT grafts produced aerial roots in four out of seven trials, correlating specifically to the presence of AtTCTP2-GFP protein in the same graft samples (see Table 1 and Figure 2). (B: right) Hence, in the samples where no aerial roots were present no protein was observed. (C) The latter is consistent to the WT/WT graft controls.

Mentions: Adventitious roots are normally found in some plant species and have a role in adaptation to stress or nutrient deficiency (Drew et al., 1979), as well as for vegetative propagation of some tree species (Naiman and Décamps, 1997). The emergence of aerial roots in the adventitious region in the graft interface was observed in several of the grafting experiments performed (Figure 5). Indeed, such adventitious roots were observed in the sites adjacent to the graft union in all AtTCTP2/WT grafts (Figure 5A), and in some of the WT/AtTCTP2 grafts (Figure 5B) but not in the control (Figure 5C). Interestingly, the appearance of these adventitious roots was specifically associated to the grafts where GFP-associated fluorescence (and hence AtTCTP2) was detected in WT scion or stock, and thus, in which long-distance transport of the protein occurred (Table 1); interestingly, in some cases fluorescence was observed, even though AtTCTP2 mRNA was detected. This suggests that the emergence of these aerial roots is linked to the movement of the protein rather than to movement of the transcript. No GFP-associated fluorescence was observed in equivalent tissues from WT homografts.


Long distance movement of an Arabidopsis Translationally Controlled Tumor Protein (AtTCTP2) mRNA and protein in tobacco.

Toscano-Morales R, Xoconostle-Cázares B, Martínez-Navarro AC, Ruiz-Medrano R - Front Plant Sci (2014)

Emergence of adventitious roots associated to long-distance movement of AtTCTP2. Representative images were taken previous tissue collection for RT-PCR and confocal analysis (30 days). (A) WT/AtTCTP2 grafts presented aerial roots in all samples, which is consistent with the protein detection in the same graft samples (see Table 1). (B: left and middle) AtTCTP2/WT grafts produced aerial roots in four out of seven trials, correlating specifically to the presence of AtTCTP2-GFP protein in the same graft samples (see Table 1 and Figure 2). (B: right) Hence, in the samples where no aerial roots were present no protein was observed. (C) The latter is consistent to the WT/WT graft controls.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4269120&req=5

Figure 5: Emergence of adventitious roots associated to long-distance movement of AtTCTP2. Representative images were taken previous tissue collection for RT-PCR and confocal analysis (30 days). (A) WT/AtTCTP2 grafts presented aerial roots in all samples, which is consistent with the protein detection in the same graft samples (see Table 1). (B: left and middle) AtTCTP2/WT grafts produced aerial roots in four out of seven trials, correlating specifically to the presence of AtTCTP2-GFP protein in the same graft samples (see Table 1 and Figure 2). (B: right) Hence, in the samples where no aerial roots were present no protein was observed. (C) The latter is consistent to the WT/WT graft controls.
Mentions: Adventitious roots are normally found in some plant species and have a role in adaptation to stress or nutrient deficiency (Drew et al., 1979), as well as for vegetative propagation of some tree species (Naiman and Décamps, 1997). The emergence of aerial roots in the adventitious region in the graft interface was observed in several of the grafting experiments performed (Figure 5). Indeed, such adventitious roots were observed in the sites adjacent to the graft union in all AtTCTP2/WT grafts (Figure 5A), and in some of the WT/AtTCTP2 grafts (Figure 5B) but not in the control (Figure 5C). Interestingly, the appearance of these adventitious roots was specifically associated to the grafts where GFP-associated fluorescence (and hence AtTCTP2) was detected in WT scion or stock, and thus, in which long-distance transport of the protein occurred (Table 1); interestingly, in some cases fluorescence was observed, even though AtTCTP2 mRNA was detected. This suggests that the emergence of these aerial roots is linked to the movement of the protein rather than to movement of the transcript. No GFP-associated fluorescence was observed in equivalent tissues from WT homografts.

Bottom Line: The results indicate that both AtTCTP2 mRNA and protein are capable of moving long distance in both directions (stock-scion and scion-stock) with a tendency for movement from source to sink tissue (stock to scion).In addition, the protein localization pattern in transgenic aerial and primary roots was basically the same, indicating specific nuclear destination in roots, but also in leaves.These findings provide an approach to understand the role of long-distance movement in the function of plant TCTPs, supporting the notion that some of these act in a non-cell autonomous manner, as the human counterpart, the Histamine Releasing Factor (HRF).

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology and Bioengineering, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional Mexico, Mexico.

ABSTRACT
Translationally Controlled Tumor Protein (TCTP) is an almost ubiquitous protein found in eukaryotes, fundamental for the regulation of development and general growth. The multiple functions of TCTP have been inferred from its involvement in several cell pathways, but the specific function of TCTP is still not known in detail. On the other hand, TCTP seems to respond to a plethora of external signals, and appears to be regulated at the transcriptional and/or translational levels by mechanisms yet to be determined. In the present work, we analyzed the capacity of AtTCTP2 gene products (mRNA and protein) to translocate long distance through tobacco heterografts (transgenic/WT and WT/transgenic). The results indicate that both AtTCTP2 mRNA and protein are capable of moving long distance in both directions (stock-scion and scion-stock) with a tendency for movement from source to sink tissue (stock to scion). Interestingly, aerial roots emerged only in heterografts where the protein was detected in both stock and scion, suggesting a correlation between the presence of AtTCTP2 and aerial root appearance. More detailed analysis showed that these aerial roots harbored the transgene and expressed both transcript and protein. In addition, the protein localization pattern in transgenic aerial and primary roots was basically the same, indicating specific nuclear destination in roots, but also in leaves. These findings provide an approach to understand the role of long-distance movement in the function of plant TCTPs, supporting the notion that some of these act in a non-cell autonomous manner, as the human counterpart, the Histamine Releasing Factor (HRF).

No MeSH data available.


Related in: MedlinePlus