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Long distance movement of an Arabidopsis Translationally Controlled Tumor Protein (AtTCTP2) mRNA and protein in tobacco.

Toscano-Morales R, Xoconostle-Cázares B, Martínez-Navarro AC, Ruiz-Medrano R - Front Plant Sci (2014)

Bottom Line: The results indicate that both AtTCTP2 mRNA and protein are capable of moving long distance in both directions (stock-scion and scion-stock) with a tendency for movement from source to sink tissue (stock to scion).In addition, the protein localization pattern in transgenic aerial and primary roots was basically the same, indicating specific nuclear destination in roots, but also in leaves.These findings provide an approach to understand the role of long-distance movement in the function of plant TCTPs, supporting the notion that some of these act in a non-cell autonomous manner, as the human counterpart, the Histamine Releasing Factor (HRF).

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology and Bioengineering, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional Mexico, Mexico.

ABSTRACT
Translationally Controlled Tumor Protein (TCTP) is an almost ubiquitous protein found in eukaryotes, fundamental for the regulation of development and general growth. The multiple functions of TCTP have been inferred from its involvement in several cell pathways, but the specific function of TCTP is still not known in detail. On the other hand, TCTP seems to respond to a plethora of external signals, and appears to be regulated at the transcriptional and/or translational levels by mechanisms yet to be determined. In the present work, we analyzed the capacity of AtTCTP2 gene products (mRNA and protein) to translocate long distance through tobacco heterografts (transgenic/WT and WT/transgenic). The results indicate that both AtTCTP2 mRNA and protein are capable of moving long distance in both directions (stock-scion and scion-stock) with a tendency for movement from source to sink tissue (stock to scion). Interestingly, aerial roots emerged only in heterografts where the protein was detected in both stock and scion, suggesting a correlation between the presence of AtTCTP2 and aerial root appearance. More detailed analysis showed that these aerial roots harbored the transgene and expressed both transcript and protein. In addition, the protein localization pattern in transgenic aerial and primary roots was basically the same, indicating specific nuclear destination in roots, but also in leaves. These findings provide an approach to understand the role of long-distance movement in the function of plant TCTPs, supporting the notion that some of these act in a non-cell autonomous manner, as the human counterpart, the Histamine Releasing Factor (HRF).

No MeSH data available.


Related in: MedlinePlus

AtTCTP2-GFP protein moves long distance in tobacco grafts. Inmunodetection was performed to detect GFP fused to AtTCTP2 from scions and stocks for each graft tested. Panels (A,C,E,F) are examples of the fusion protein long-distance movement (AtTCTP2-GFP = 50 kDa) from rootstock to scion. Panel (B) is the unique example of AtTCTP2-GFP long distance transport from scion to rootstock. Panel (D) is a representative example of lack of AtTCTP2-GFP long-distance movement from scion to rootstock.
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Figure 3: AtTCTP2-GFP protein moves long distance in tobacco grafts. Inmunodetection was performed to detect GFP fused to AtTCTP2 from scions and stocks for each graft tested. Panels (A,C,E,F) are examples of the fusion protein long-distance movement (AtTCTP2-GFP = 50 kDa) from rootstock to scion. Panel (B) is the unique example of AtTCTP2-GFP long distance transport from scion to rootstock. Panel (D) is a representative example of lack of AtTCTP2-GFP long-distance movement from scion to rootstock.

Mentions: The previous results could not discriminate whether the AtTCTP2-GFP transcript, the protein, or both, were able to move across the graft union. In order to answer this question, the presence of the fusion protein was determined by Western blot analysis of total proteins from scions and stocks of transgenic plants. Independent scions and stocks were used in which AtTCTP2 mRNA movement was observed. In all cases, the fusion protein was detected in both WT scion grafted onto a transgenic stock; in the case of WT stock onto which a transgenic scion had been grafted, one out of two AtTCTP2 could be detected in the former (Figure 3). These results indicate that AtTCTP2-GFP moves long distance as both mRNA and protein, and that such movement is not always in the direction from source to sink.


Long distance movement of an Arabidopsis Translationally Controlled Tumor Protein (AtTCTP2) mRNA and protein in tobacco.

Toscano-Morales R, Xoconostle-Cázares B, Martínez-Navarro AC, Ruiz-Medrano R - Front Plant Sci (2014)

AtTCTP2-GFP protein moves long distance in tobacco grafts. Inmunodetection was performed to detect GFP fused to AtTCTP2 from scions and stocks for each graft tested. Panels (A,C,E,F) are examples of the fusion protein long-distance movement (AtTCTP2-GFP = 50 kDa) from rootstock to scion. Panel (B) is the unique example of AtTCTP2-GFP long distance transport from scion to rootstock. Panel (D) is a representative example of lack of AtTCTP2-GFP long-distance movement from scion to rootstock.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4269120&req=5

Figure 3: AtTCTP2-GFP protein moves long distance in tobacco grafts. Inmunodetection was performed to detect GFP fused to AtTCTP2 from scions and stocks for each graft tested. Panels (A,C,E,F) are examples of the fusion protein long-distance movement (AtTCTP2-GFP = 50 kDa) from rootstock to scion. Panel (B) is the unique example of AtTCTP2-GFP long distance transport from scion to rootstock. Panel (D) is a representative example of lack of AtTCTP2-GFP long-distance movement from scion to rootstock.
Mentions: The previous results could not discriminate whether the AtTCTP2-GFP transcript, the protein, or both, were able to move across the graft union. In order to answer this question, the presence of the fusion protein was determined by Western blot analysis of total proteins from scions and stocks of transgenic plants. Independent scions and stocks were used in which AtTCTP2 mRNA movement was observed. In all cases, the fusion protein was detected in both WT scion grafted onto a transgenic stock; in the case of WT stock onto which a transgenic scion had been grafted, one out of two AtTCTP2 could be detected in the former (Figure 3). These results indicate that AtTCTP2-GFP moves long distance as both mRNA and protein, and that such movement is not always in the direction from source to sink.

Bottom Line: The results indicate that both AtTCTP2 mRNA and protein are capable of moving long distance in both directions (stock-scion and scion-stock) with a tendency for movement from source to sink tissue (stock to scion).In addition, the protein localization pattern in transgenic aerial and primary roots was basically the same, indicating specific nuclear destination in roots, but also in leaves.These findings provide an approach to understand the role of long-distance movement in the function of plant TCTPs, supporting the notion that some of these act in a non-cell autonomous manner, as the human counterpart, the Histamine Releasing Factor (HRF).

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology and Bioengineering, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional Mexico, Mexico.

ABSTRACT
Translationally Controlled Tumor Protein (TCTP) is an almost ubiquitous protein found in eukaryotes, fundamental for the regulation of development and general growth. The multiple functions of TCTP have been inferred from its involvement in several cell pathways, but the specific function of TCTP is still not known in detail. On the other hand, TCTP seems to respond to a plethora of external signals, and appears to be regulated at the transcriptional and/or translational levels by mechanisms yet to be determined. In the present work, we analyzed the capacity of AtTCTP2 gene products (mRNA and protein) to translocate long distance through tobacco heterografts (transgenic/WT and WT/transgenic). The results indicate that both AtTCTP2 mRNA and protein are capable of moving long distance in both directions (stock-scion and scion-stock) with a tendency for movement from source to sink tissue (stock to scion). Interestingly, aerial roots emerged only in heterografts where the protein was detected in both stock and scion, suggesting a correlation between the presence of AtTCTP2 and aerial root appearance. More detailed analysis showed that these aerial roots harbored the transgene and expressed both transcript and protein. In addition, the protein localization pattern in transgenic aerial and primary roots was basically the same, indicating specific nuclear destination in roots, but also in leaves. These findings provide an approach to understand the role of long-distance movement in the function of plant TCTPs, supporting the notion that some of these act in a non-cell autonomous manner, as the human counterpart, the Histamine Releasing Factor (HRF).

No MeSH data available.


Related in: MedlinePlus