Limits...
The impact of sulfate restriction on seed yield and quality of winter oilseed rape depends on the ability to remobilize sulfate from vegetative tissues to reproductive organs.

Girondé A, Dubousset L, Trouverie J, Etienne P, Avice JC - Front Plant Sci (2014)

Bottom Line: In response to S limitation at the bolting stage, the seed yield and quality were dramatically reduced compared to control plants.When S limitation occurred at the early flowering stage, oilseed rape can optimize the mobilization of sulfate reserves from vegetative organs (leaves and stem) to satisfy the demand of seeds and maintain the seed yield and quality.Our study also revealed that the stem may act as a transient storage organ for remobilized S coming from source leaves before its utilization by seeds.

View Article: PubMed Central - PubMed

Affiliation: Normandie University Caen, France ; Université de Caen Basse Normandie, UMR INRA-UCBN 950 Ecophysiologie Végétale, Agronomie and Nutritions N.C.S. Caen, France ; Institut National de la Recherche Agronomique, UMR INRA-UCBN 950 Ecophysiologie Végétale, Agronomie and Nutritions N.C.S. Caen, France.

ABSTRACT
Our current knowledge about sulfur (S) management by winter oilseed rape to satisfy the S demand of developing seeds is still scarce, particularly in relation to S restriction. Our goals were to determine the physiological processes related to S use efficiency that led to maintain the seed yield and quality when S limitation occurred at the bolting or early flowering stages. To address these questions, a pulse-chase (34)SO(2-) 4 labeling method was carried out in order to study the S fluxes from uptake and remobilization at the whole plant level. In response of S limitation at the bolting or early flowering stages, the leaves are the most important source organ for S remobilization during reproductive stages. By combining (34)S-tracer with biochemical fractionation in order to separate sulfate from other S-compounds, it appeared that sulfate was the main form of S remobilized in leaves at reproductive stages and that tonoplastic SULTR4-type transporters were specifically involved in the sulfate remobilisation in case of low S availability. In response to S limitation at the bolting stage, the seed yield and quality were dramatically reduced compared to control plants. These data suggest that the increase of both S remobilization from source leaves and the root proliferation in order to maximize sulfate uptake capacities, were not sufficient to maintain the seed yield and quality. When S limitation occurred at the early flowering stage, oilseed rape can optimize the mobilization of sulfate reserves from vegetative organs (leaves and stem) to satisfy the demand of seeds and maintain the seed yield and quality. Our study also revealed that the stem may act as a transient storage organ for remobilized S coming from source leaves before its utilization by seeds. The physiological traits (S remobilization, root proliferation, transient S storage in stem) observed under S limitation could be used in breeding programs to select oilseed rape genotypes with high S use efficiency.

No MeSH data available.


Evolution of the dry matter (A), changes in the S amount (B) and seed yield and composition (C) of oilseed rape subjected to limitation of sulfate at the early flowering stage. S limitation (LS53 plants supplied with 8.7 μM sulfate) was compared to control plants (HS plants supplied with 508.7 μM sulfate). (A) Variations in dry matter of LS53 plants (as % of HS plants) between GS70 (start of pod filling) and GS81 (seed coloring). (B) Variations in the S amount in different organs of LS53 plants (as % of HS plants) between GS70 and GS81. A positive or a negative value indicates that the dry matter or the S amount is increased or reduced compared with HS plants, respectively. (C) Seed yield and composition of seeds (proteins, glucosinolates, oil, fatty acids) in LS53 plants (as % of HS plants) at mature seed stage (GS99). Significant differences between treatments are indicated with asterisks (n = 4; *P < 0.05; **P < 0.01).
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Figure 5: Evolution of the dry matter (A), changes in the S amount (B) and seed yield and composition (C) of oilseed rape subjected to limitation of sulfate at the early flowering stage. S limitation (LS53 plants supplied with 8.7 μM sulfate) was compared to control plants (HS plants supplied with 508.7 μM sulfate). (A) Variations in dry matter of LS53 plants (as % of HS plants) between GS70 (start of pod filling) and GS81 (seed coloring). (B) Variations in the S amount in different organs of LS53 plants (as % of HS plants) between GS70 and GS81. A positive or a negative value indicates that the dry matter or the S amount is increased or reduced compared with HS plants, respectively. (C) Seed yield and composition of seeds (proteins, glucosinolates, oil, fatty acids) in LS53 plants (as % of HS plants) at mature seed stage (GS99). Significant differences between treatments are indicated with asterisks (n = 4; *P < 0.05; **P < 0.01).

Mentions: Compared to control (HS plants), a limitation of sulfate occurring at the early flowering stage (LS53 plants) had no effect on dry matter of all organs except for dry matter of leaves at GS81 (-61.3% compared to HS, Figure 5A). The S amount of LS53 plants is lower than HS plants in all organs at every growing stage, except for stem at GS70 and roots at GS81, for which there were no significant differences compared to HS plants (Figure 5B). The seed dry matter per plant (Figure 5C) was not affected by S limitation. In addition, protein content was not altered, in contrast to the glucosinolate content, which decreased by 82% compared to HS plants (Figure 5C). Oil content and oil quality were also slightly reduced in LS plants (P < 0.05) due to the decrease in linoleic and linolenic acid content (−9.4 and −11.5%, respectively, compared to HS plants) (Figure 5C).


The impact of sulfate restriction on seed yield and quality of winter oilseed rape depends on the ability to remobilize sulfate from vegetative tissues to reproductive organs.

Girondé A, Dubousset L, Trouverie J, Etienne P, Avice JC - Front Plant Sci (2014)

Evolution of the dry matter (A), changes in the S amount (B) and seed yield and composition (C) of oilseed rape subjected to limitation of sulfate at the early flowering stage. S limitation (LS53 plants supplied with 8.7 μM sulfate) was compared to control plants (HS plants supplied with 508.7 μM sulfate). (A) Variations in dry matter of LS53 plants (as % of HS plants) between GS70 (start of pod filling) and GS81 (seed coloring). (B) Variations in the S amount in different organs of LS53 plants (as % of HS plants) between GS70 and GS81. A positive or a negative value indicates that the dry matter or the S amount is increased or reduced compared with HS plants, respectively. (C) Seed yield and composition of seeds (proteins, glucosinolates, oil, fatty acids) in LS53 plants (as % of HS plants) at mature seed stage (GS99). Significant differences between treatments are indicated with asterisks (n = 4; *P < 0.05; **P < 0.01).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4269117&req=5

Figure 5: Evolution of the dry matter (A), changes in the S amount (B) and seed yield and composition (C) of oilseed rape subjected to limitation of sulfate at the early flowering stage. S limitation (LS53 plants supplied with 8.7 μM sulfate) was compared to control plants (HS plants supplied with 508.7 μM sulfate). (A) Variations in dry matter of LS53 plants (as % of HS plants) between GS70 (start of pod filling) and GS81 (seed coloring). (B) Variations in the S amount in different organs of LS53 plants (as % of HS plants) between GS70 and GS81. A positive or a negative value indicates that the dry matter or the S amount is increased or reduced compared with HS plants, respectively. (C) Seed yield and composition of seeds (proteins, glucosinolates, oil, fatty acids) in LS53 plants (as % of HS plants) at mature seed stage (GS99). Significant differences between treatments are indicated with asterisks (n = 4; *P < 0.05; **P < 0.01).
Mentions: Compared to control (HS plants), a limitation of sulfate occurring at the early flowering stage (LS53 plants) had no effect on dry matter of all organs except for dry matter of leaves at GS81 (-61.3% compared to HS, Figure 5A). The S amount of LS53 plants is lower than HS plants in all organs at every growing stage, except for stem at GS70 and roots at GS81, for which there were no significant differences compared to HS plants (Figure 5B). The seed dry matter per plant (Figure 5C) was not affected by S limitation. In addition, protein content was not altered, in contrast to the glucosinolate content, which decreased by 82% compared to HS plants (Figure 5C). Oil content and oil quality were also slightly reduced in LS plants (P < 0.05) due to the decrease in linoleic and linolenic acid content (−9.4 and −11.5%, respectively, compared to HS plants) (Figure 5C).

Bottom Line: In response to S limitation at the bolting stage, the seed yield and quality were dramatically reduced compared to control plants.When S limitation occurred at the early flowering stage, oilseed rape can optimize the mobilization of sulfate reserves from vegetative organs (leaves and stem) to satisfy the demand of seeds and maintain the seed yield and quality.Our study also revealed that the stem may act as a transient storage organ for remobilized S coming from source leaves before its utilization by seeds.

View Article: PubMed Central - PubMed

Affiliation: Normandie University Caen, France ; Université de Caen Basse Normandie, UMR INRA-UCBN 950 Ecophysiologie Végétale, Agronomie and Nutritions N.C.S. Caen, France ; Institut National de la Recherche Agronomique, UMR INRA-UCBN 950 Ecophysiologie Végétale, Agronomie and Nutritions N.C.S. Caen, France.

ABSTRACT
Our current knowledge about sulfur (S) management by winter oilseed rape to satisfy the S demand of developing seeds is still scarce, particularly in relation to S restriction. Our goals were to determine the physiological processes related to S use efficiency that led to maintain the seed yield and quality when S limitation occurred at the bolting or early flowering stages. To address these questions, a pulse-chase (34)SO(2-) 4 labeling method was carried out in order to study the S fluxes from uptake and remobilization at the whole plant level. In response of S limitation at the bolting or early flowering stages, the leaves are the most important source organ for S remobilization during reproductive stages. By combining (34)S-tracer with biochemical fractionation in order to separate sulfate from other S-compounds, it appeared that sulfate was the main form of S remobilized in leaves at reproductive stages and that tonoplastic SULTR4-type transporters were specifically involved in the sulfate remobilisation in case of low S availability. In response to S limitation at the bolting stage, the seed yield and quality were dramatically reduced compared to control plants. These data suggest that the increase of both S remobilization from source leaves and the root proliferation in order to maximize sulfate uptake capacities, were not sufficient to maintain the seed yield and quality. When S limitation occurred at the early flowering stage, oilseed rape can optimize the mobilization of sulfate reserves from vegetative organs (leaves and stem) to satisfy the demand of seeds and maintain the seed yield and quality. Our study also revealed that the stem may act as a transient storage organ for remobilized S coming from source leaves before its utilization by seeds. The physiological traits (S remobilization, root proliferation, transient S storage in stem) observed under S limitation could be used in breeding programs to select oilseed rape genotypes with high S use efficiency.

No MeSH data available.