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Transcript profiles of maize embryo sacs and preliminary identification of genes involved in the embryo sac-pollen tube interaction.

Wang SS, Wang F, Tan SJ, Wang MX, Sui N, Zhang XS - Front Plant Sci (2014)

Bottom Line: Additionally, our analyses indicate that the expression of 112 genes encoding cysteine-rich proteins (CRPs) is induced during pollination and fertilization.The CRPs likely regulate pollen tube guidance and embryo sac development.These results provide important information on the genes involved in the embryo sac-pollen tube interaction in maize.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University Tai'an, China.

ABSTRACT
The embryo sac, the female gametophyte of flowering plants, plays important roles in the pollination and fertilization process. Maize (Zea mays L.) is a model monocot, but little is known about the interactions between its embryo sac and the pollen tube. In this study, we compared the transcript profiles of mature embryo sacs, mature embryo sacs 14-16 h after pollination, and mature nucelli. Comparing the transcript profiles of the embryo sacs before and after the entry of the pollen tube, we identified 3467 differentially expressed transcripts (3382 differentially expressed genes; DEGs). The DEGs were grouped into 22 functional categories. Among the DEGs, 221 genes were induced upon the entry of the pollen tube, and many of them encoded proteins involved in RNA binding, processing, and transcription, signaling, miscellaneous enzyme family processes, and lipid metabolism processes. Genes in the DEG dataset were grouped into 17 classes in a gene ontology enrichment analysis. The DEGs included many genes encoding proteins involved in protein amino acid phosphorylation and protein ubiquitination, implying that these processes might play important roles in the embryo sac-pollen tube interaction. Additionally, our analyses indicate that the expression of 112 genes encoding cysteine-rich proteins (CRPs) is induced during pollination and fertilization. The CRPs likely regulate pollen tube guidance and embryo sac development. These results provide important information on the genes involved in the embryo sac-pollen tube interaction in maize.

No MeSH data available.


Related in: MedlinePlus

Classification of genes induced by pollination. Using MapMan, 221 genes induced by pollination were classified into 14 categories; details of genes involved in lipid metabolism and signaling are shown on the left.
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Figure 5: Classification of genes induced by pollination. Using MapMan, 221 genes induced by pollination were classified into 14 categories; details of genes involved in lipid metabolism and signaling are shown on the left.

Mentions: Interestingly, 221 transcripts were expressed exclusively in the ESP (Additional file 10). We defined these 221 transcripts as those that were induced by the entry of the pollen tube. These genes may encode proteins that function in the pollen–embryo sac interaction in maize. Using MapMan software, the 221 transcripts were grouped into 11 functional categories (Figure 5). As shown in Figure 5, the largest category was un-annotated genes (25%), followed by genes involved in RNA binding, processing and transcription (12%), signaling (10%), miscellaneous enzyme family processes (10%) and lipid metabolism (6%).


Transcript profiles of maize embryo sacs and preliminary identification of genes involved in the embryo sac-pollen tube interaction.

Wang SS, Wang F, Tan SJ, Wang MX, Sui N, Zhang XS - Front Plant Sci (2014)

Classification of genes induced by pollination. Using MapMan, 221 genes induced by pollination were classified into 14 categories; details of genes involved in lipid metabolism and signaling are shown on the left.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4269116&req=5

Figure 5: Classification of genes induced by pollination. Using MapMan, 221 genes induced by pollination were classified into 14 categories; details of genes involved in lipid metabolism and signaling are shown on the left.
Mentions: Interestingly, 221 transcripts were expressed exclusively in the ESP (Additional file 10). We defined these 221 transcripts as those that were induced by the entry of the pollen tube. These genes may encode proteins that function in the pollen–embryo sac interaction in maize. Using MapMan software, the 221 transcripts were grouped into 11 functional categories (Figure 5). As shown in Figure 5, the largest category was un-annotated genes (25%), followed by genes involved in RNA binding, processing and transcription (12%), signaling (10%), miscellaneous enzyme family processes (10%) and lipid metabolism (6%).

Bottom Line: Additionally, our analyses indicate that the expression of 112 genes encoding cysteine-rich proteins (CRPs) is induced during pollination and fertilization.The CRPs likely regulate pollen tube guidance and embryo sac development.These results provide important information on the genes involved in the embryo sac-pollen tube interaction in maize.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University Tai'an, China.

ABSTRACT
The embryo sac, the female gametophyte of flowering plants, plays important roles in the pollination and fertilization process. Maize (Zea mays L.) is a model monocot, but little is known about the interactions between its embryo sac and the pollen tube. In this study, we compared the transcript profiles of mature embryo sacs, mature embryo sacs 14-16 h after pollination, and mature nucelli. Comparing the transcript profiles of the embryo sacs before and after the entry of the pollen tube, we identified 3467 differentially expressed transcripts (3382 differentially expressed genes; DEGs). The DEGs were grouped into 22 functional categories. Among the DEGs, 221 genes were induced upon the entry of the pollen tube, and many of them encoded proteins involved in RNA binding, processing, and transcription, signaling, miscellaneous enzyme family processes, and lipid metabolism processes. Genes in the DEG dataset were grouped into 17 classes in a gene ontology enrichment analysis. The DEGs included many genes encoding proteins involved in protein amino acid phosphorylation and protein ubiquitination, implying that these processes might play important roles in the embryo sac-pollen tube interaction. Additionally, our analyses indicate that the expression of 112 genes encoding cysteine-rich proteins (CRPs) is induced during pollination and fertilization. The CRPs likely regulate pollen tube guidance and embryo sac development. These results provide important information on the genes involved in the embryo sac-pollen tube interaction in maize.

No MeSH data available.


Related in: MedlinePlus