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Transcript profiles of maize embryo sacs and preliminary identification of genes involved in the embryo sac-pollen tube interaction.

Wang SS, Wang F, Tan SJ, Wang MX, Sui N, Zhang XS - Front Plant Sci (2014)

Bottom Line: Additionally, our analyses indicate that the expression of 112 genes encoding cysteine-rich proteins (CRPs) is induced during pollination and fertilization.The CRPs likely regulate pollen tube guidance and embryo sac development.These results provide important information on the genes involved in the embryo sac-pollen tube interaction in maize.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University Tai'an, China.

ABSTRACT
The embryo sac, the female gametophyte of flowering plants, plays important roles in the pollination and fertilization process. Maize (Zea mays L.) is a model monocot, but little is known about the interactions between its embryo sac and the pollen tube. In this study, we compared the transcript profiles of mature embryo sacs, mature embryo sacs 14-16 h after pollination, and mature nucelli. Comparing the transcript profiles of the embryo sacs before and after the entry of the pollen tube, we identified 3467 differentially expressed transcripts (3382 differentially expressed genes; DEGs). The DEGs were grouped into 22 functional categories. Among the DEGs, 221 genes were induced upon the entry of the pollen tube, and many of them encoded proteins involved in RNA binding, processing, and transcription, signaling, miscellaneous enzyme family processes, and lipid metabolism processes. Genes in the DEG dataset were grouped into 17 classes in a gene ontology enrichment analysis. The DEGs included many genes encoding proteins involved in protein amino acid phosphorylation and protein ubiquitination, implying that these processes might play important roles in the embryo sac-pollen tube interaction. Additionally, our analyses indicate that the expression of 112 genes encoding cysteine-rich proteins (CRPs) is induced during pollination and fertilization. The CRPs likely regulate pollen tube guidance and embryo sac development. These results provide important information on the genes involved in the embryo sac-pollen tube interaction in maize.

No MeSH data available.


Related in: MedlinePlus

Venn diagram showing number of transcripts detected in ES, ESP, and Nu.
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Figure 2: Venn diagram showing number of transcripts detected in ES, ESP, and Nu.

Mentions: To identify the genes corresponding to the reads in each library, the filtered clean reads were mapped to version 2 of the maize B73 reference genome (AGPv2, http://www.maizesequence.org) using SOAP2 software. To make the libraries meaningful, transcripts with RPKM values of less than 1 were eliminated before further statistical analyses. Finally, 20,005 transcripts (18,257 genes) in the ES, 22,148 transcripts (19,354 genes) in the Nu, and 17,420 transcripts (16,503 genes) in the ESP were detected (Figure 2, Additional file 5). Among the 20,005 transcripts detected in the ES, approximately 80% (15,925/20,005) were also detected in the ESP. Of them, 809 transcripts (803 genes) were specifically expressed in the ESP (Figure 2, Additional file 6). In previous studies, many genes have been identified in cell-specific groups of embryo sac (Lê et al., 2005; Yu et al., 2005; Yang et al., 2006; Jones-Rhoades et al., 2007; Wuest et al., 2010; Chettoor et al., 2014). Compared with those data, we identified homologs of 176 genes in other species, which were specifically expressed in egg, central cell or synergids (Additional file 6). The information in this study might provide the important gene resources for the network underlying the embryo sac-pollen tube interaction.


Transcript profiles of maize embryo sacs and preliminary identification of genes involved in the embryo sac-pollen tube interaction.

Wang SS, Wang F, Tan SJ, Wang MX, Sui N, Zhang XS - Front Plant Sci (2014)

Venn diagram showing number of transcripts detected in ES, ESP, and Nu.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4269116&req=5

Figure 2: Venn diagram showing number of transcripts detected in ES, ESP, and Nu.
Mentions: To identify the genes corresponding to the reads in each library, the filtered clean reads were mapped to version 2 of the maize B73 reference genome (AGPv2, http://www.maizesequence.org) using SOAP2 software. To make the libraries meaningful, transcripts with RPKM values of less than 1 were eliminated before further statistical analyses. Finally, 20,005 transcripts (18,257 genes) in the ES, 22,148 transcripts (19,354 genes) in the Nu, and 17,420 transcripts (16,503 genes) in the ESP were detected (Figure 2, Additional file 5). Among the 20,005 transcripts detected in the ES, approximately 80% (15,925/20,005) were also detected in the ESP. Of them, 809 transcripts (803 genes) were specifically expressed in the ESP (Figure 2, Additional file 6). In previous studies, many genes have been identified in cell-specific groups of embryo sac (Lê et al., 2005; Yu et al., 2005; Yang et al., 2006; Jones-Rhoades et al., 2007; Wuest et al., 2010; Chettoor et al., 2014). Compared with those data, we identified homologs of 176 genes in other species, which were specifically expressed in egg, central cell or synergids (Additional file 6). The information in this study might provide the important gene resources for the network underlying the embryo sac-pollen tube interaction.

Bottom Line: Additionally, our analyses indicate that the expression of 112 genes encoding cysteine-rich proteins (CRPs) is induced during pollination and fertilization.The CRPs likely regulate pollen tube guidance and embryo sac development.These results provide important information on the genes involved in the embryo sac-pollen tube interaction in maize.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University Tai'an, China.

ABSTRACT
The embryo sac, the female gametophyte of flowering plants, plays important roles in the pollination and fertilization process. Maize (Zea mays L.) is a model monocot, but little is known about the interactions between its embryo sac and the pollen tube. In this study, we compared the transcript profiles of mature embryo sacs, mature embryo sacs 14-16 h after pollination, and mature nucelli. Comparing the transcript profiles of the embryo sacs before and after the entry of the pollen tube, we identified 3467 differentially expressed transcripts (3382 differentially expressed genes; DEGs). The DEGs were grouped into 22 functional categories. Among the DEGs, 221 genes were induced upon the entry of the pollen tube, and many of them encoded proteins involved in RNA binding, processing, and transcription, signaling, miscellaneous enzyme family processes, and lipid metabolism processes. Genes in the DEG dataset were grouped into 17 classes in a gene ontology enrichment analysis. The DEGs included many genes encoding proteins involved in protein amino acid phosphorylation and protein ubiquitination, implying that these processes might play important roles in the embryo sac-pollen tube interaction. Additionally, our analyses indicate that the expression of 112 genes encoding cysteine-rich proteins (CRPs) is induced during pollination and fertilization. The CRPs likely regulate pollen tube guidance and embryo sac development. These results provide important information on the genes involved in the embryo sac-pollen tube interaction in maize.

No MeSH data available.


Related in: MedlinePlus