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Transcript profiles of maize embryo sacs and preliminary identification of genes involved in the embryo sac-pollen tube interaction.

Wang SS, Wang F, Tan SJ, Wang MX, Sui N, Zhang XS - Front Plant Sci (2014)

Bottom Line: Additionally, our analyses indicate that the expression of 112 genes encoding cysteine-rich proteins (CRPs) is induced during pollination and fertilization.The CRPs likely regulate pollen tube guidance and embryo sac development.These results provide important information on the genes involved in the embryo sac-pollen tube interaction in maize.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University Tai'an, China.

ABSTRACT
The embryo sac, the female gametophyte of flowering plants, plays important roles in the pollination and fertilization process. Maize (Zea mays L.) is a model monocot, but little is known about the interactions between its embryo sac and the pollen tube. In this study, we compared the transcript profiles of mature embryo sacs, mature embryo sacs 14-16 h after pollination, and mature nucelli. Comparing the transcript profiles of the embryo sacs before and after the entry of the pollen tube, we identified 3467 differentially expressed transcripts (3382 differentially expressed genes; DEGs). The DEGs were grouped into 22 functional categories. Among the DEGs, 221 genes were induced upon the entry of the pollen tube, and many of them encoded proteins involved in RNA binding, processing, and transcription, signaling, miscellaneous enzyme family processes, and lipid metabolism processes. Genes in the DEG dataset were grouped into 17 classes in a gene ontology enrichment analysis. The DEGs included many genes encoding proteins involved in protein amino acid phosphorylation and protein ubiquitination, implying that these processes might play important roles in the embryo sac-pollen tube interaction. Additionally, our analyses indicate that the expression of 112 genes encoding cysteine-rich proteins (CRPs) is induced during pollination and fertilization. The CRPs likely regulate pollen tube guidance and embryo sac development. These results provide important information on the genes involved in the embryo sac-pollen tube interaction in maize.

No MeSH data available.


Related in: MedlinePlus

Samples used for RNA-seq analyses. (A) ES with an egg; (B) ESP with a central cell, male nucleus (mn) moving to female nucleus (arrowheads). a, antipodal cells; en, egg nucleus; pn, polar nucleus; n, nucellus. Scale bars = 50 μm.
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Figure 1: Samples used for RNA-seq analyses. (A) ES with an egg; (B) ESP with a central cell, male nucleus (mn) moving to female nucleus (arrowheads). a, antipodal cells; en, egg nucleus; pn, polar nucleus; n, nucellus. Scale bars = 50 μm.

Mentions: In the maize cultivar Q319, the time from pollination until the arrival of the pollen tube at the embryo sac is approximately 14 h. Therefore, we identified the ES (embryo sac at anthesis) and the ESP (embryo sac at 14 h after pollination) by laser scanning confocal microscope observation. In the ES, the egg nucleus was present (Figure 1A). In the ESP, the male nucleus was close to the polar nucleus. It suggested that the female and male gametes interact in the ESP (Figure 1B).


Transcript profiles of maize embryo sacs and preliminary identification of genes involved in the embryo sac-pollen tube interaction.

Wang SS, Wang F, Tan SJ, Wang MX, Sui N, Zhang XS - Front Plant Sci (2014)

Samples used for RNA-seq analyses. (A) ES with an egg; (B) ESP with a central cell, male nucleus (mn) moving to female nucleus (arrowheads). a, antipodal cells; en, egg nucleus; pn, polar nucleus; n, nucellus. Scale bars = 50 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4269116&req=5

Figure 1: Samples used for RNA-seq analyses. (A) ES with an egg; (B) ESP with a central cell, male nucleus (mn) moving to female nucleus (arrowheads). a, antipodal cells; en, egg nucleus; pn, polar nucleus; n, nucellus. Scale bars = 50 μm.
Mentions: In the maize cultivar Q319, the time from pollination until the arrival of the pollen tube at the embryo sac is approximately 14 h. Therefore, we identified the ES (embryo sac at anthesis) and the ESP (embryo sac at 14 h after pollination) by laser scanning confocal microscope observation. In the ES, the egg nucleus was present (Figure 1A). In the ESP, the male nucleus was close to the polar nucleus. It suggested that the female and male gametes interact in the ESP (Figure 1B).

Bottom Line: Additionally, our analyses indicate that the expression of 112 genes encoding cysteine-rich proteins (CRPs) is induced during pollination and fertilization.The CRPs likely regulate pollen tube guidance and embryo sac development.These results provide important information on the genes involved in the embryo sac-pollen tube interaction in maize.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University Tai'an, China.

ABSTRACT
The embryo sac, the female gametophyte of flowering plants, plays important roles in the pollination and fertilization process. Maize (Zea mays L.) is a model monocot, but little is known about the interactions between its embryo sac and the pollen tube. In this study, we compared the transcript profiles of mature embryo sacs, mature embryo sacs 14-16 h after pollination, and mature nucelli. Comparing the transcript profiles of the embryo sacs before and after the entry of the pollen tube, we identified 3467 differentially expressed transcripts (3382 differentially expressed genes; DEGs). The DEGs were grouped into 22 functional categories. Among the DEGs, 221 genes were induced upon the entry of the pollen tube, and many of them encoded proteins involved in RNA binding, processing, and transcription, signaling, miscellaneous enzyme family processes, and lipid metabolism processes. Genes in the DEG dataset were grouped into 17 classes in a gene ontology enrichment analysis. The DEGs included many genes encoding proteins involved in protein amino acid phosphorylation and protein ubiquitination, implying that these processes might play important roles in the embryo sac-pollen tube interaction. Additionally, our analyses indicate that the expression of 112 genes encoding cysteine-rich proteins (CRPs) is induced during pollination and fertilization. The CRPs likely regulate pollen tube guidance and embryo sac development. These results provide important information on the genes involved in the embryo sac-pollen tube interaction in maize.

No MeSH data available.


Related in: MedlinePlus