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Whole Genome Expression Profiling and Signal Pathway Screening of MSCs in Ankylosing Spondylitis.

Li Y, Wang P, Xie Z, Huang L, Yang R, Gao L, Tang Y, Zhang X, Ye J, Chen K, Cai Z, Wu Y, Shen H - Stem Cells Int (2014)

Bottom Line: Our results showed that in normal media 676 genes were differentially expressed in AS, 354 upregulated and 322 downregulated, while in an inflammatory environment 1767 genes were differentially expressed in AS, 1230 upregulated and 537 downregulated.In addition, by KEGG pathway analysis, 14 key genes from the MAPK signaling and 8 key genes from the TLR signaling pathway were identified as differentially regulated.The results of qRT-PCR verified the expression variation of the 9 genes mentioned above.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, No. 107 Yanjiang Road West, Guangzhou 510120, China.

ABSTRACT
The pathogenesis of dysfunctional immunoregulation of mesenchymal stem cells (MSCs) in ankylosing spondylitis (AS) is thought to be a complex process that involves multiple genetic alterations. In this study, MSCs derived from both healthy donors and AS patients were cultured in normal media or media mimicking an inflammatory environment. Whole genome expression profiling analysis of 33,351 genes was performed and differentially expressed genes related to AS were analyzed by GO term analysis and KEGG pathway analysis. Our results showed that in normal media 676 genes were differentially expressed in AS, 354 upregulated and 322 downregulated, while in an inflammatory environment 1767 genes were differentially expressed in AS, 1230 upregulated and 537 downregulated. GO analysis showed that these genes were mainly related to cellular processes, physiological processes, biological regulation, regulation of biological processes, and binding. In addition, by KEGG pathway analysis, 14 key genes from the MAPK signaling and 8 key genes from the TLR signaling pathway were identified as differentially regulated. The results of qRT-PCR verified the expression variation of the 9 genes mentioned above. Our study found that in an inflammatory environment ankylosing spondylitis pathogenesis may be related to activation of the MAPK and TLR signaling pathways.

No MeSH data available.


Related in: MedlinePlus

Whole genomic expression analysis of MSCs in AS patients. (a) shows the scatter plot of differentially expressed genes of MSCs from both healthy donors and AS patients, respectively, in both normal and inflammatory culture environment. The values of x and y axes in the scatter plot are the normalized signal values of each sample (log2 scaled). The green lines are fold change lines (the default fold change value given is 2.0). Genes above the top green line and below the bottom green line indicated more than 2.0-fold change of genes between two compared arrays. (b) shows the pie chart of gene ontology (GO) analysis of results of the differentially expressed genes of MSCs between group AS-Infla and group HD-Infla. Among the 1590 genes, 892 (56.10%) participated in biological process, 461 (28.99%) played a role in molecular function, and 237 (14.91%) associated with cellular component.
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fig2: Whole genomic expression analysis of MSCs in AS patients. (a) shows the scatter plot of differentially expressed genes of MSCs from both healthy donors and AS patients, respectively, in both normal and inflammatory culture environment. The values of x and y axes in the scatter plot are the normalized signal values of each sample (log2 scaled). The green lines are fold change lines (the default fold change value given is 2.0). Genes above the top green line and below the bottom green line indicated more than 2.0-fold change of genes between two compared arrays. (b) shows the pie chart of gene ontology (GO) analysis of results of the differentially expressed genes of MSCs between group AS-Infla and group HD-Infla. Among the 1590 genes, 892 (56.10%) participated in biological process, 461 (28.99%) played a role in molecular function, and 237 (14.91%) associated with cellular component.

Mentions: The experimental system was stable and fluorescent signal intensity was strong and homogenous. When cultured under inflammatory conditions, the number of differentially expressed genes in MSCs from AS patients was increased (Figure 2(a)). There were 676 differentially expressed genes in the AS-Norm group, of which 354 were upregulated and 322 were downregulated as compared to the HD-Norm group. 1767 genes were differentially expressed in the AS-Infla group, 1230 upregulated and 537 downregulated, as compared to the HD-Infla group. We used GO (gene ontology) to analyze these differentially expressed genes within three domains (Figure 2(b)).


Whole Genome Expression Profiling and Signal Pathway Screening of MSCs in Ankylosing Spondylitis.

Li Y, Wang P, Xie Z, Huang L, Yang R, Gao L, Tang Y, Zhang X, Ye J, Chen K, Cai Z, Wu Y, Shen H - Stem Cells Int (2014)

Whole genomic expression analysis of MSCs in AS patients. (a) shows the scatter plot of differentially expressed genes of MSCs from both healthy donors and AS patients, respectively, in both normal and inflammatory culture environment. The values of x and y axes in the scatter plot are the normalized signal values of each sample (log2 scaled). The green lines are fold change lines (the default fold change value given is 2.0). Genes above the top green line and below the bottom green line indicated more than 2.0-fold change of genes between two compared arrays. (b) shows the pie chart of gene ontology (GO) analysis of results of the differentially expressed genes of MSCs between group AS-Infla and group HD-Infla. Among the 1590 genes, 892 (56.10%) participated in biological process, 461 (28.99%) played a role in molecular function, and 237 (14.91%) associated with cellular component.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4269092&req=5

fig2: Whole genomic expression analysis of MSCs in AS patients. (a) shows the scatter plot of differentially expressed genes of MSCs from both healthy donors and AS patients, respectively, in both normal and inflammatory culture environment. The values of x and y axes in the scatter plot are the normalized signal values of each sample (log2 scaled). The green lines are fold change lines (the default fold change value given is 2.0). Genes above the top green line and below the bottom green line indicated more than 2.0-fold change of genes between two compared arrays. (b) shows the pie chart of gene ontology (GO) analysis of results of the differentially expressed genes of MSCs between group AS-Infla and group HD-Infla. Among the 1590 genes, 892 (56.10%) participated in biological process, 461 (28.99%) played a role in molecular function, and 237 (14.91%) associated with cellular component.
Mentions: The experimental system was stable and fluorescent signal intensity was strong and homogenous. When cultured under inflammatory conditions, the number of differentially expressed genes in MSCs from AS patients was increased (Figure 2(a)). There were 676 differentially expressed genes in the AS-Norm group, of which 354 were upregulated and 322 were downregulated as compared to the HD-Norm group. 1767 genes were differentially expressed in the AS-Infla group, 1230 upregulated and 537 downregulated, as compared to the HD-Infla group. We used GO (gene ontology) to analyze these differentially expressed genes within three domains (Figure 2(b)).

Bottom Line: Our results showed that in normal media 676 genes were differentially expressed in AS, 354 upregulated and 322 downregulated, while in an inflammatory environment 1767 genes were differentially expressed in AS, 1230 upregulated and 537 downregulated.In addition, by KEGG pathway analysis, 14 key genes from the MAPK signaling and 8 key genes from the TLR signaling pathway were identified as differentially regulated.The results of qRT-PCR verified the expression variation of the 9 genes mentioned above.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, No. 107 Yanjiang Road West, Guangzhou 510120, China.

ABSTRACT
The pathogenesis of dysfunctional immunoregulation of mesenchymal stem cells (MSCs) in ankylosing spondylitis (AS) is thought to be a complex process that involves multiple genetic alterations. In this study, MSCs derived from both healthy donors and AS patients were cultured in normal media or media mimicking an inflammatory environment. Whole genome expression profiling analysis of 33,351 genes was performed and differentially expressed genes related to AS were analyzed by GO term analysis and KEGG pathway analysis. Our results showed that in normal media 676 genes were differentially expressed in AS, 354 upregulated and 322 downregulated, while in an inflammatory environment 1767 genes were differentially expressed in AS, 1230 upregulated and 537 downregulated. GO analysis showed that these genes were mainly related to cellular processes, physiological processes, biological regulation, regulation of biological processes, and binding. In addition, by KEGG pathway analysis, 14 key genes from the MAPK signaling and 8 key genes from the TLR signaling pathway were identified as differentially regulated. The results of qRT-PCR verified the expression variation of the 9 genes mentioned above. Our study found that in an inflammatory environment ankylosing spondylitis pathogenesis may be related to activation of the MAPK and TLR signaling pathways.

No MeSH data available.


Related in: MedlinePlus