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Whole Genome Expression Profiling and Signal Pathway Screening of MSCs in Ankylosing Spondylitis.

Li Y, Wang P, Xie Z, Huang L, Yang R, Gao L, Tang Y, Zhang X, Ye J, Chen K, Cai Z, Wu Y, Shen H - Stem Cells Int (2014)

Bottom Line: Our results showed that in normal media 676 genes were differentially expressed in AS, 354 upregulated and 322 downregulated, while in an inflammatory environment 1767 genes were differentially expressed in AS, 1230 upregulated and 537 downregulated.In addition, by KEGG pathway analysis, 14 key genes from the MAPK signaling and 8 key genes from the TLR signaling pathway were identified as differentially regulated.The results of qRT-PCR verified the expression variation of the 9 genes mentioned above.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, No. 107 Yanjiang Road West, Guangzhou 510120, China.

ABSTRACT
The pathogenesis of dysfunctional immunoregulation of mesenchymal stem cells (MSCs) in ankylosing spondylitis (AS) is thought to be a complex process that involves multiple genetic alterations. In this study, MSCs derived from both healthy donors and AS patients were cultured in normal media or media mimicking an inflammatory environment. Whole genome expression profiling analysis of 33,351 genes was performed and differentially expressed genes related to AS were analyzed by GO term analysis and KEGG pathway analysis. Our results showed that in normal media 676 genes were differentially expressed in AS, 354 upregulated and 322 downregulated, while in an inflammatory environment 1767 genes were differentially expressed in AS, 1230 upregulated and 537 downregulated. GO analysis showed that these genes were mainly related to cellular processes, physiological processes, biological regulation, regulation of biological processes, and binding. In addition, by KEGG pathway analysis, 14 key genes from the MAPK signaling and 8 key genes from the TLR signaling pathway were identified as differentially regulated. The results of qRT-PCR verified the expression variation of the 9 genes mentioned above. Our study found that in an inflammatory environment ankylosing spondylitis pathogenesis may be related to activation of the MAPK and TLR signaling pathways.

No MeSH data available.


Related in: MedlinePlus

Immunophenotype and differentiation of MSCs. (a) Under the 40x microscope, spindle-shaped MSCs grow like intersecting bundles. (b) Dot plot data showing all (nongated) cells as well as gated hMSC (P1). (c) MSCs were harvested, labeled with antibodies against immunophenotype, and analyzed by flow cytometry. MSCs expressed CD29, CD44, and CD105 but not CD34, CD45, and HLA-DR.
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fig1: Immunophenotype and differentiation of MSCs. (a) Under the 40x microscope, spindle-shaped MSCs grow like intersecting bundles. (b) Dot plot data showing all (nongated) cells as well as gated hMSC (P1). (c) MSCs were harvested, labeled with antibodies against immunophenotype, and analyzed by flow cytometry. MSCs expressed CD29, CD44, and CD105 but not CD34, CD45, and HLA-DR.

Mentions: MSCs were obtained from bone marrow aspirates of both healthy volunteers and AS patients. MSCs were cultured and purified to homogeneity according to the culture method described in our previous work [9]. Figure 1(a) shows adherent MSCs growing like intersecting spindle bundles. In addition, immunophenotyping was performed by flow cytometry. Dot plots showing all (ungated) cells as well as gated MSCs are displayed in Figure 1(b). MSCs constitutively expressed CD29, CD44, and CD105 but not CD34, CD45, or HLA-DR (Figure 1(c)).


Whole Genome Expression Profiling and Signal Pathway Screening of MSCs in Ankylosing Spondylitis.

Li Y, Wang P, Xie Z, Huang L, Yang R, Gao L, Tang Y, Zhang X, Ye J, Chen K, Cai Z, Wu Y, Shen H - Stem Cells Int (2014)

Immunophenotype and differentiation of MSCs. (a) Under the 40x microscope, spindle-shaped MSCs grow like intersecting bundles. (b) Dot plot data showing all (nongated) cells as well as gated hMSC (P1). (c) MSCs were harvested, labeled with antibodies against immunophenotype, and analyzed by flow cytometry. MSCs expressed CD29, CD44, and CD105 but not CD34, CD45, and HLA-DR.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4269092&req=5

fig1: Immunophenotype and differentiation of MSCs. (a) Under the 40x microscope, spindle-shaped MSCs grow like intersecting bundles. (b) Dot plot data showing all (nongated) cells as well as gated hMSC (P1). (c) MSCs were harvested, labeled with antibodies against immunophenotype, and analyzed by flow cytometry. MSCs expressed CD29, CD44, and CD105 but not CD34, CD45, and HLA-DR.
Mentions: MSCs were obtained from bone marrow aspirates of both healthy volunteers and AS patients. MSCs were cultured and purified to homogeneity according to the culture method described in our previous work [9]. Figure 1(a) shows adherent MSCs growing like intersecting spindle bundles. In addition, immunophenotyping was performed by flow cytometry. Dot plots showing all (ungated) cells as well as gated MSCs are displayed in Figure 1(b). MSCs constitutively expressed CD29, CD44, and CD105 but not CD34, CD45, or HLA-DR (Figure 1(c)).

Bottom Line: Our results showed that in normal media 676 genes were differentially expressed in AS, 354 upregulated and 322 downregulated, while in an inflammatory environment 1767 genes were differentially expressed in AS, 1230 upregulated and 537 downregulated.In addition, by KEGG pathway analysis, 14 key genes from the MAPK signaling and 8 key genes from the TLR signaling pathway were identified as differentially regulated.The results of qRT-PCR verified the expression variation of the 9 genes mentioned above.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, No. 107 Yanjiang Road West, Guangzhou 510120, China.

ABSTRACT
The pathogenesis of dysfunctional immunoregulation of mesenchymal stem cells (MSCs) in ankylosing spondylitis (AS) is thought to be a complex process that involves multiple genetic alterations. In this study, MSCs derived from both healthy donors and AS patients were cultured in normal media or media mimicking an inflammatory environment. Whole genome expression profiling analysis of 33,351 genes was performed and differentially expressed genes related to AS were analyzed by GO term analysis and KEGG pathway analysis. Our results showed that in normal media 676 genes were differentially expressed in AS, 354 upregulated and 322 downregulated, while in an inflammatory environment 1767 genes were differentially expressed in AS, 1230 upregulated and 537 downregulated. GO analysis showed that these genes were mainly related to cellular processes, physiological processes, biological regulation, regulation of biological processes, and binding. In addition, by KEGG pathway analysis, 14 key genes from the MAPK signaling and 8 key genes from the TLR signaling pathway were identified as differentially regulated. The results of qRT-PCR verified the expression variation of the 9 genes mentioned above. Our study found that in an inflammatory environment ankylosing spondylitis pathogenesis may be related to activation of the MAPK and TLR signaling pathways.

No MeSH data available.


Related in: MedlinePlus