Limits...
Fibroblast growth factor 18 increases the trophic effects of bone marrow mesenchymal stem cells on chondrocytes isolated from late stage osteoarthritic patients.

Zhang Z, Wang Y, Li M, Li J, Wu J - Stem Cells Int (2014)

Bottom Line: Finally, coimplantation of MSCs with OA chondrocytes produces more matrix than chondrocytes only.In conclusion, FGF18 can restore the responsiveness of OA chondrocytes to the trophic effects of MSCs.Coimplantation of MSCs and OA chondrocytes treated with FGF18 may be a good alternative cell source for regenerating cartilage tissue that is degraded during OA pathological changes.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, The First Affiliated Hospital of Harbin Medical University, 23 You Zheng Street, Nangang District, Harbin 150001, China.

ABSTRACT
Coculture of mesenchymal stem cells with chondrocytes increases production of cartilaginous matrix. Chondrocytes isolated from late stage osteoarthritic patients usually lost their phenotype of producing cartilaginous matrix. Fibroblast growth factor 18 is believed to redifferentiate OA chondrocyte into functionally active chondrocytes. The aim of this study is to investigate the supportive effects of MSCs on OA chondrocytes and test if FGF18 could enhance the responsiveness of OA chondrocytes to the support of MSCs in a coculture system. Both pellet and transwell co-cultures were used. GAG quantification, hydroxyproline assay, and qPCR were performed. An ectopic models of cartilage formation was also applied. Our data indicated that, in pellets coculture of MSCs and OA chondrocytes, matrix production was increased in the presence of FGF18, comparing to the monoculture of chondrocytes. Results from transwell coculture study showed that expression of matrix producing genes in OA chondrocytes increased when cocultured with MSCs with FGF18 in culture medium, while hypertrophic genes were not changed by coculture. Finally, coimplantation of MSCs with OA chondrocytes produces more matrix than chondrocytes only. In conclusion, FGF18 can restore the responsiveness of OA chondrocytes to the trophic effects of MSCs. Coimplantation of MSCs and OA chondrocytes treated with FGF18 may be a good alternative cell source for regenerating cartilage tissue that is degraded during OA pathological changes.

No MeSH data available.


Related in: MedlinePlus

Expression of chondrogenic genes was measured by qPCR in OA chondrocytes cultured in transwells. (a) Diagram shows how monoculture or coculture is performed in transwell systems. (b) Expression of chondrogenic genes in chondrocytes cultured in transwells in medium without FGF18. Beta-actin was used for normalization. Monoculture was chosen as reference. Number in coculture represents the relative expression level of corresponding gene comparing to monoculture. Three donor pairs were analyzed. NS: nonsignificance. (c) Expression of chondrogenic genes in chondrocytes cultured in transwells in medium containing FGF18 (10 ng/mL). Beta-actin was used for normalization. Monoculture was chosen as reference. Number in coculture represents the relative expression level of corresponding gene comparing to monoculture. Three donor pairs were analyzed. P values are calculated with Student's t-test.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4269084&req=5

fig2: Expression of chondrogenic genes was measured by qPCR in OA chondrocytes cultured in transwells. (a) Diagram shows how monoculture or coculture is performed in transwell systems. (b) Expression of chondrogenic genes in chondrocytes cultured in transwells in medium without FGF18. Beta-actin was used for normalization. Monoculture was chosen as reference. Number in coculture represents the relative expression level of corresponding gene comparing to monoculture. Three donor pairs were analyzed. NS: nonsignificance. (c) Expression of chondrogenic genes in chondrocytes cultured in transwells in medium containing FGF18 (10 ng/mL). Beta-actin was used for normalization. Monoculture was chosen as reference. Number in coculture represents the relative expression level of corresponding gene comparing to monoculture. Three donor pairs were analyzed. P values are calculated with Student's t-test.

Mentions: For transwell coculture, 100 000 chondrocytes were seeded in one Millicell cell culture insert (0.45 μm PCF, 12 mm Diameter) manufactured by Merk Millipore (Tullagreen, Ireland). Cell culture insert was then put in one well of 24-well plate where 100 000 MSCs were seeded. Serum-free medium with or without FGF18 (10 ng/mL) was used. For monoculture transwells, 100 000 chondrocytes were seeded in cell culture insert and one well of a 24-well plate, respectively. This is illustrated in Figure 2(a).


Fibroblast growth factor 18 increases the trophic effects of bone marrow mesenchymal stem cells on chondrocytes isolated from late stage osteoarthritic patients.

Zhang Z, Wang Y, Li M, Li J, Wu J - Stem Cells Int (2014)

Expression of chondrogenic genes was measured by qPCR in OA chondrocytes cultured in transwells. (a) Diagram shows how monoculture or coculture is performed in transwell systems. (b) Expression of chondrogenic genes in chondrocytes cultured in transwells in medium without FGF18. Beta-actin was used for normalization. Monoculture was chosen as reference. Number in coculture represents the relative expression level of corresponding gene comparing to monoculture. Three donor pairs were analyzed. NS: nonsignificance. (c) Expression of chondrogenic genes in chondrocytes cultured in transwells in medium containing FGF18 (10 ng/mL). Beta-actin was used for normalization. Monoculture was chosen as reference. Number in coculture represents the relative expression level of corresponding gene comparing to monoculture. Three donor pairs were analyzed. P values are calculated with Student's t-test.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4269084&req=5

fig2: Expression of chondrogenic genes was measured by qPCR in OA chondrocytes cultured in transwells. (a) Diagram shows how monoculture or coculture is performed in transwell systems. (b) Expression of chondrogenic genes in chondrocytes cultured in transwells in medium without FGF18. Beta-actin was used for normalization. Monoculture was chosen as reference. Number in coculture represents the relative expression level of corresponding gene comparing to monoculture. Three donor pairs were analyzed. NS: nonsignificance. (c) Expression of chondrogenic genes in chondrocytes cultured in transwells in medium containing FGF18 (10 ng/mL). Beta-actin was used for normalization. Monoculture was chosen as reference. Number in coculture represents the relative expression level of corresponding gene comparing to monoculture. Three donor pairs were analyzed. P values are calculated with Student's t-test.
Mentions: For transwell coculture, 100 000 chondrocytes were seeded in one Millicell cell culture insert (0.45 μm PCF, 12 mm Diameter) manufactured by Merk Millipore (Tullagreen, Ireland). Cell culture insert was then put in one well of 24-well plate where 100 000 MSCs were seeded. Serum-free medium with or without FGF18 (10 ng/mL) was used. For monoculture transwells, 100 000 chondrocytes were seeded in cell culture insert and one well of a 24-well plate, respectively. This is illustrated in Figure 2(a).

Bottom Line: Finally, coimplantation of MSCs with OA chondrocytes produces more matrix than chondrocytes only.In conclusion, FGF18 can restore the responsiveness of OA chondrocytes to the trophic effects of MSCs.Coimplantation of MSCs and OA chondrocytes treated with FGF18 may be a good alternative cell source for regenerating cartilage tissue that is degraded during OA pathological changes.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, The First Affiliated Hospital of Harbin Medical University, 23 You Zheng Street, Nangang District, Harbin 150001, China.

ABSTRACT
Coculture of mesenchymal stem cells with chondrocytes increases production of cartilaginous matrix. Chondrocytes isolated from late stage osteoarthritic patients usually lost their phenotype of producing cartilaginous matrix. Fibroblast growth factor 18 is believed to redifferentiate OA chondrocyte into functionally active chondrocytes. The aim of this study is to investigate the supportive effects of MSCs on OA chondrocytes and test if FGF18 could enhance the responsiveness of OA chondrocytes to the support of MSCs in a coculture system. Both pellet and transwell co-cultures were used. GAG quantification, hydroxyproline assay, and qPCR were performed. An ectopic models of cartilage formation was also applied. Our data indicated that, in pellets coculture of MSCs and OA chondrocytes, matrix production was increased in the presence of FGF18, comparing to the monoculture of chondrocytes. Results from transwell coculture study showed that expression of matrix producing genes in OA chondrocytes increased when cocultured with MSCs with FGF18 in culture medium, while hypertrophic genes were not changed by coculture. Finally, coimplantation of MSCs with OA chondrocytes produces more matrix than chondrocytes only. In conclusion, FGF18 can restore the responsiveness of OA chondrocytes to the trophic effects of MSCs. Coimplantation of MSCs and OA chondrocytes treated with FGF18 may be a good alternative cell source for regenerating cartilage tissue that is degraded during OA pathological changes.

No MeSH data available.


Related in: MedlinePlus