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Clinical response and transfusion reactions of sheep subjected to single homologous blood transfusion.

Sousa RS, Minervino AH, Araújo CA, Rodrigues FA, Oliveira FL, Mori CS, Zaminhan JL, Moreira TR, Sousa IK, Ortolani EL, Barrêto RA - ScientificWorldJournal (2014)

Bottom Line: Eighteen adult sheep were subjected to a single phlebotomy to withdraw 40% of the total blood volume, which was placed into CPDA-1 bags and then divided into G0, animals that received fresh blood, and G15 and G35, animals that received blood stored for 15 or 35 days, respectively.All groups presented transfusion reactions, among which hyperthermia was the most frequent (50% of animals).Tachycardia occurred most frequently in the G35 animals (50% of them).

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Science, Faculty of Veterinary Medicine, University of Sao Paulo, Avenida Professor Orlando Marques de Paiva, 87 Cidade Universitária, 05508-270 São Paulo, SP, Brazil.

ABSTRACT
Studies in relation to blood conservation and responses to transfusion are scarce for ruminants. We evaluated the clinical manifestations of sheep that received a single homologous transfusion of whole blood, focusing on transfusion reactions. Eighteen adult sheep were subjected to a single phlebotomy to withdraw 40% of the total blood volume, which was placed into CPDA-1 bags and then divided into G0, animals that received fresh blood, and G15 and G35, animals that received blood stored for 15 or 35 days, respectively. Clinical observations were recorded throughout the transfusion, whereas heart rate, respiratory rate, and rectal temperature were assessed at the following times: 24 hours after phlebotomy and before transfusion; 30 minutes, six, twelve, 24, 48, 72, and 96 hours and eight and 16 days after transfusion. All groups presented transfusion reactions, among which hyperthermia was the most frequent (50% of animals). Tachycardia occurred most frequently in the G35 animals (50% of them). During transfusion G35 animals presented more clinical manifestation (P < 0.05). Transfusion of fresh or stored total blood improved the blood volume, but transfusion reactions occurred, demonstrating that a single transfusion of fresh or stored blood can cause inflammatory and febrile nonhemolytic transfusion reactions in sheep.

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Related in: MedlinePlus

Mean and standard deviation of the heart rate (beats/min), respiratory rate (breaths/minute), and rectal temperature (°C) of sheep after transfusion with whole blood fresh or stored for 15 or 35 days at the time points analyzed. ∗ Mean statistical difference in comparison with T0 (P < 0.05) in each group. Different capital letters mean difference between experimental groups in one specific moment (P < 0.05).
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Related In: Results  -  Collection


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fig1: Mean and standard deviation of the heart rate (beats/min), respiratory rate (breaths/minute), and rectal temperature (°C) of sheep after transfusion with whole blood fresh or stored for 15 or 35 days at the time points analyzed. ∗ Mean statistical difference in comparison with T0 (P < 0.05) in each group. Different capital letters mean difference between experimental groups in one specific moment (P < 0.05).

Mentions: Figure 1 presents the mean values and standard deviations for heart rate, respiratory rate and rectal temperature. There were no significant differences between the three groups in relation to heart rate and respiratory rate. In comparing between the times, there was a reduction in G0 (P < 0.05) in heart rate at T96 and T16 d when compared to T0. The mean temperature in G0 was greater than G15 (P < 0.05) at T96.


Clinical response and transfusion reactions of sheep subjected to single homologous blood transfusion.

Sousa RS, Minervino AH, Araújo CA, Rodrigues FA, Oliveira FL, Mori CS, Zaminhan JL, Moreira TR, Sousa IK, Ortolani EL, Barrêto RA - ScientificWorldJournal (2014)

Mean and standard deviation of the heart rate (beats/min), respiratory rate (breaths/minute), and rectal temperature (°C) of sheep after transfusion with whole blood fresh or stored for 15 or 35 days at the time points analyzed. ∗ Mean statistical difference in comparison with T0 (P < 0.05) in each group. Different capital letters mean difference between experimental groups in one specific moment (P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4269081&req=5

fig1: Mean and standard deviation of the heart rate (beats/min), respiratory rate (breaths/minute), and rectal temperature (°C) of sheep after transfusion with whole blood fresh or stored for 15 or 35 days at the time points analyzed. ∗ Mean statistical difference in comparison with T0 (P < 0.05) in each group. Different capital letters mean difference between experimental groups in one specific moment (P < 0.05).
Mentions: Figure 1 presents the mean values and standard deviations for heart rate, respiratory rate and rectal temperature. There were no significant differences between the three groups in relation to heart rate and respiratory rate. In comparing between the times, there was a reduction in G0 (P < 0.05) in heart rate at T96 and T16 d when compared to T0. The mean temperature in G0 was greater than G15 (P < 0.05) at T96.

Bottom Line: Eighteen adult sheep were subjected to a single phlebotomy to withdraw 40% of the total blood volume, which was placed into CPDA-1 bags and then divided into G0, animals that received fresh blood, and G15 and G35, animals that received blood stored for 15 or 35 days, respectively.All groups presented transfusion reactions, among which hyperthermia was the most frequent (50% of animals).Tachycardia occurred most frequently in the G35 animals (50% of them).

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Science, Faculty of Veterinary Medicine, University of Sao Paulo, Avenida Professor Orlando Marques de Paiva, 87 Cidade Universitária, 05508-270 São Paulo, SP, Brazil.

ABSTRACT
Studies in relation to blood conservation and responses to transfusion are scarce for ruminants. We evaluated the clinical manifestations of sheep that received a single homologous transfusion of whole blood, focusing on transfusion reactions. Eighteen adult sheep were subjected to a single phlebotomy to withdraw 40% of the total blood volume, which was placed into CPDA-1 bags and then divided into G0, animals that received fresh blood, and G15 and G35, animals that received blood stored for 15 or 35 days, respectively. Clinical observations were recorded throughout the transfusion, whereas heart rate, respiratory rate, and rectal temperature were assessed at the following times: 24 hours after phlebotomy and before transfusion; 30 minutes, six, twelve, 24, 48, 72, and 96 hours and eight and 16 days after transfusion. All groups presented transfusion reactions, among which hyperthermia was the most frequent (50% of animals). Tachycardia occurred most frequently in the G35 animals (50% of them). During transfusion G35 animals presented more clinical manifestation (P < 0.05). Transfusion of fresh or stored total blood improved the blood volume, but transfusion reactions occurred, demonstrating that a single transfusion of fresh or stored blood can cause inflammatory and febrile nonhemolytic transfusion reactions in sheep.

Show MeSH
Related in: MedlinePlus