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Ultrafast diffusion of a fluorescent cholesterol analog in compartmentalized plasma membranes.

Hiramoto-Yamaki N, Tanaka KA, Suzuki KG, Hirosawa KM, Miyahara MS, Kalay Z, Tanaka K, Kasai RS, Kusumi A, Fujiwara TK - Traffic (2014)

Bottom Line: Surprisingly, in the intact PM, Bdp-Chol diffused at the fastest rate ever found for any molecules in the PM, with a median diffusion coefficient (D) of 3.4 µm²/second, which was ∼10 times greater than that of non-raft phospholipid molecules (0.33 µm²/second), despite Bdp-Chol's probable association with raft domains.These results are consistent with the previously proposed model, in which the PM is compartmentalized by the actin-based membrane-skeleton fence and its associated transmembrane picket proteins for the macroscopic diffusion of all of the membrane molecules, and suggest that the probability of Bdp-Chol passing through the compartment boundaries, once it enters the boundary, is ∼10× greater than that of Cy3-DOPE.Since the compartment sizes are greater than those of the putative raft domains, we conclude that raft domains coexist with membrane-skeleton-induced compartments and are contained within them.

View Article: PubMed Central - PubMed

Affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS) and Institute for Frontier Medical Sciences, Kyoto University, Kyoto, 606-8507, Japan.

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The effects of partial actin depolymerization and stabilization on the compartment size of the HASM-cell PM, evaluated by ultrafast single-particle tracking of Gold-PEs at a 20-microsecond resolution. Arrowheads indicate median values. Gray bars indicate the distributions before drug treatments (control). The results are summarized in Table4, including the numbers of examined particles. p: the results of the Mann–Whitney U-test.
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fig08: The effects of partial actin depolymerization and stabilization on the compartment size of the HASM-cell PM, evaluated by ultrafast single-particle tracking of Gold-PEs at a 20-microsecond resolution. Arrowheads indicate median values. Gray bars indicate the distributions before drug treatments (control). The results are summarized in Table4, including the numbers of examined particles. p: the results of the Mann–Whitney U-test.

Mentions: The treatment with latrunculin-B induced ∼2-fold increases in the compartment size (evaluated by ultrafast single gold-particle tracking using Gold-PEs; Figure 8, left column, Table4). In contrast, as described in the previous subsection, it modestly increased the DeffMACRO values of Cy3-PEs by a factor of ∼1.2 (Table3).


Ultrafast diffusion of a fluorescent cholesterol analog in compartmentalized plasma membranes.

Hiramoto-Yamaki N, Tanaka KA, Suzuki KG, Hirosawa KM, Miyahara MS, Kalay Z, Tanaka K, Kasai RS, Kusumi A, Fujiwara TK - Traffic (2014)

The effects of partial actin depolymerization and stabilization on the compartment size of the HASM-cell PM, evaluated by ultrafast single-particle tracking of Gold-PEs at a 20-microsecond resolution. Arrowheads indicate median values. Gray bars indicate the distributions before drug treatments (control). The results are summarized in Table4, including the numbers of examined particles. p: the results of the Mann–Whitney U-test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4265843&req=5

fig08: The effects of partial actin depolymerization and stabilization on the compartment size of the HASM-cell PM, evaluated by ultrafast single-particle tracking of Gold-PEs at a 20-microsecond resolution. Arrowheads indicate median values. Gray bars indicate the distributions before drug treatments (control). The results are summarized in Table4, including the numbers of examined particles. p: the results of the Mann–Whitney U-test.
Mentions: The treatment with latrunculin-B induced ∼2-fold increases in the compartment size (evaluated by ultrafast single gold-particle tracking using Gold-PEs; Figure 8, left column, Table4). In contrast, as described in the previous subsection, it modestly increased the DeffMACRO values of Cy3-PEs by a factor of ∼1.2 (Table3).

Bottom Line: Surprisingly, in the intact PM, Bdp-Chol diffused at the fastest rate ever found for any molecules in the PM, with a median diffusion coefficient (D) of 3.4 µm²/second, which was ∼10 times greater than that of non-raft phospholipid molecules (0.33 µm²/second), despite Bdp-Chol's probable association with raft domains.These results are consistent with the previously proposed model, in which the PM is compartmentalized by the actin-based membrane-skeleton fence and its associated transmembrane picket proteins for the macroscopic diffusion of all of the membrane molecules, and suggest that the probability of Bdp-Chol passing through the compartment boundaries, once it enters the boundary, is ∼10× greater than that of Cy3-DOPE.Since the compartment sizes are greater than those of the putative raft domains, we conclude that raft domains coexist with membrane-skeleton-induced compartments and are contained within them.

View Article: PubMed Central - PubMed

Affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS) and Institute for Frontier Medical Sciences, Kyoto University, Kyoto, 606-8507, Japan.

Show MeSH