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Resazurin tube method: rapid, simple, and inexpensive method for detection of drug resistance in the clinical isolates of mycobacterium tuberculosis.

Patil SS, Mohite ST, Kulkarni SA, Udgaonkar US - J Glob Infect Dis (2014)

Bottom Line: Rapid detection is critical for the effective treatment of patients.The results obtained were compared with the gold standard proportion method.Kappa is the measure of agreement between the RTM and proportion method (PM) for RIF and INH, which was found to be 0.972 and 0.935 for RIF and INH, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Bharati Vidyapeeth Deemed University, Medical College and Hospital, Sangli, Maharashtra, India.

ABSTRACT

Background: Tuberculosis (TB) remains a serious public health problem worldwide. The emergence of drug resistance and multidrug resistance (MDR) has become the main threat to TB treatment and control programs. Rapid detection is critical for the effective treatment of patients. In recent times, a new method using the colorimetric indicator resazurin has been proposed for drug susceptibility of Mycobacterium tuberculosis.

Materials and methods: In this study, the resazurin reduction assay was adapted to screw cap tubes. Using the Resazurin Tube Method (RTM), a total of 100 clinical isolates were tested against Rifampicin (RIF) and Isoniazide (INH). By visual reading, the minimum inhibitory concentrations (MICs) were obtained after eight days. The results obtained were compared with the gold standard proportion method.

Results: Excellent results were obtained for RTM with a sensitivity of 100% for both RIF and INH, with a specificity of 98.7 and 95.3%, respectively. Kappa is the measure of agreement between the RTM and proportion method (PM) for RIF and INH, which was found to be 0.972 and 0.935 for RIF and INH, respectively.

Conclusion: The RTM appears to be a reliable method for the rapid and simultaneous detection of MDR-TB and drug susceptibility testing (DST) of M. tuberculosis. It is simple, inexpensive, and with no biohazard risk involved.

No MeSH data available.


Related in: MedlinePlus

Drug sensitivity testing of MDR Mycobacterium tuberculosis strain by RTM, showing resistance to INH and RIF with MICs >1.0 μg/ml and 2.0 μg/ml, respectively. (PC: Positive control; NC: Negative control)
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Figure 2: Drug sensitivity testing of MDR Mycobacterium tuberculosis strain by RTM, showing resistance to INH and RIF with MICs >1.0 μg/ml and 2.0 μg/ml, respectively. (PC: Positive control; NC: Negative control)

Mentions: Nineteen strains resistant to both RIF and INH were categorized as MultiDrug Resistant (MDR) strains. According to RTM, the MICs for RIF and INH were ≥ 0.5 μg/ml and ≥ 0.25 μg/ml, respectively [Figure 2].


Resazurin tube method: rapid, simple, and inexpensive method for detection of drug resistance in the clinical isolates of mycobacterium tuberculosis.

Patil SS, Mohite ST, Kulkarni SA, Udgaonkar US - J Glob Infect Dis (2014)

Drug sensitivity testing of MDR Mycobacterium tuberculosis strain by RTM, showing resistance to INH and RIF with MICs >1.0 μg/ml and 2.0 μg/ml, respectively. (PC: Positive control; NC: Negative control)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4265830&req=5

Figure 2: Drug sensitivity testing of MDR Mycobacterium tuberculosis strain by RTM, showing resistance to INH and RIF with MICs >1.0 μg/ml and 2.0 μg/ml, respectively. (PC: Positive control; NC: Negative control)
Mentions: Nineteen strains resistant to both RIF and INH were categorized as MultiDrug Resistant (MDR) strains. According to RTM, the MICs for RIF and INH were ≥ 0.5 μg/ml and ≥ 0.25 μg/ml, respectively [Figure 2].

Bottom Line: Rapid detection is critical for the effective treatment of patients.The results obtained were compared with the gold standard proportion method.Kappa is the measure of agreement between the RTM and proportion method (PM) for RIF and INH, which was found to be 0.972 and 0.935 for RIF and INH, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Bharati Vidyapeeth Deemed University, Medical College and Hospital, Sangli, Maharashtra, India.

ABSTRACT

Background: Tuberculosis (TB) remains a serious public health problem worldwide. The emergence of drug resistance and multidrug resistance (MDR) has become the main threat to TB treatment and control programs. Rapid detection is critical for the effective treatment of patients. In recent times, a new method using the colorimetric indicator resazurin has been proposed for drug susceptibility of Mycobacterium tuberculosis.

Materials and methods: In this study, the resazurin reduction assay was adapted to screw cap tubes. Using the Resazurin Tube Method (RTM), a total of 100 clinical isolates were tested against Rifampicin (RIF) and Isoniazide (INH). By visual reading, the minimum inhibitory concentrations (MICs) were obtained after eight days. The results obtained were compared with the gold standard proportion method.

Results: Excellent results were obtained for RTM with a sensitivity of 100% for both RIF and INH, with a specificity of 98.7 and 95.3%, respectively. Kappa is the measure of agreement between the RTM and proportion method (PM) for RIF and INH, which was found to be 0.972 and 0.935 for RIF and INH, respectively.

Conclusion: The RTM appears to be a reliable method for the rapid and simultaneous detection of MDR-TB and drug susceptibility testing (DST) of M. tuberculosis. It is simple, inexpensive, and with no biohazard risk involved.

No MeSH data available.


Related in: MedlinePlus