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Association between changes in reproductive activity and D-glucose metabolism in the tephritid fruit fly, Bactrocera dorsalis (Hendel).

Cheng D, Chen L, Yi C, Liang G, Xu Y - Sci Rep (2014)

Bottom Line: The D-glucose levels were significantly higher in A12HM group.Maltase D expression level was significantly higher in males reared with sucrose.Body weights of the males reared with D-glucose and sucrose were significantly higher than those of the males reared with yeast extract.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Insect Ecology, College of Natural Resources and the Environment, South China Agricultural University, Guangzhou 510640, China.

ABSTRACT
Reproduction is an important life process in insects; however, few studies have attempted to demonstrate the association between reproductive activity and energy metabolism. To address this problem, we focused on the reproductive changes in Bactrocera dorsalis males. We analyzed B. dorsalis male gene expression profiles during mating (DM), 3 h after mating (A3HM) and 12 h after mating (A12HM). Gene annotation and pathway analyses of differentially expressed genes show that galactose metabolism and the starch and sucrose metabolism pathway activities were significantly higher in A12HM group. Moreover, the maltase D gene was the most strongly up-regulated gene. The D-glucose levels were significantly higher in A12HM group. Maltase D expression level was significantly higher in males reared with sucrose. Body weights of the males reared with D-glucose and sucrose were significantly higher than those of the males reared with yeast extract. We observed more mated males from the groups fed sucrose and D-glucose than from those fed yeast extract. The D-glucose levels in individual males were highest at 18:00 h, when flies exhibit the most active mating behavior. This study shows that the maltase D gene and D-glucose are the critical gene and substrate, respectively, in male B. dorsalis mating process.

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Analysis of differentially expressed genes among the treatments.Figure 2a: genes that were differentially expressed between A3HM and DM; Figure 2b: genes that were differentially expressed between A12HM and DM; Figure 2c: genes that were differentially expressed between A3HM and A12HM.
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f2: Analysis of differentially expressed genes among the treatments.Figure 2a: genes that were differentially expressed between A3HM and DM; Figure 2b: genes that were differentially expressed between A12HM and DM; Figure 2c: genes that were differentially expressed between A3HM and A12HM.

Mentions: The numbers of differentially expressed genes that were generated for A3HM vs. DM, A12HM vs. DM and A12HM vs. A3HM were 15 (4 down-regulated, 11 up-regulated), 115 (34 down-regulated, 81 up-regulated) and 72 (22 down-regulated, 50 up-regulated), respectively (Figure 2a, 2b, and 2c; see Supplementary Datasets 1, 2 and 3). A Venn diagram shows that 39 of the differentially expressed genes were shared between A12HM vs. DM and A12HM vs. A3HM; 6 differentially expressed genes were shared between A3HM vs. DM and A12HM vs. DM; 2 differentially expressed genes were shared between A12HM vs. A3HM and A3HM vs. DM; and 1 differentially expressed gene was shared between A12HM vs. DM, A12HM vs. A3HM and A3HM vs. DM. Maltase D (accession number: KM115582) was the only differentially expressed gene that was shared in all of the groups (Figure 3, Supplementary Table S1).


Association between changes in reproductive activity and D-glucose metabolism in the tephritid fruit fly, Bactrocera dorsalis (Hendel).

Cheng D, Chen L, Yi C, Liang G, Xu Y - Sci Rep (2014)

Analysis of differentially expressed genes among the treatments.Figure 2a: genes that were differentially expressed between A3HM and DM; Figure 2b: genes that were differentially expressed between A12HM and DM; Figure 2c: genes that were differentially expressed between A3HM and A12HM.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4265777&req=5

f2: Analysis of differentially expressed genes among the treatments.Figure 2a: genes that were differentially expressed between A3HM and DM; Figure 2b: genes that were differentially expressed between A12HM and DM; Figure 2c: genes that were differentially expressed between A3HM and A12HM.
Mentions: The numbers of differentially expressed genes that were generated for A3HM vs. DM, A12HM vs. DM and A12HM vs. A3HM were 15 (4 down-regulated, 11 up-regulated), 115 (34 down-regulated, 81 up-regulated) and 72 (22 down-regulated, 50 up-regulated), respectively (Figure 2a, 2b, and 2c; see Supplementary Datasets 1, 2 and 3). A Venn diagram shows that 39 of the differentially expressed genes were shared between A12HM vs. DM and A12HM vs. A3HM; 6 differentially expressed genes were shared between A3HM vs. DM and A12HM vs. DM; 2 differentially expressed genes were shared between A12HM vs. A3HM and A3HM vs. DM; and 1 differentially expressed gene was shared between A12HM vs. DM, A12HM vs. A3HM and A3HM vs. DM. Maltase D (accession number: KM115582) was the only differentially expressed gene that was shared in all of the groups (Figure 3, Supplementary Table S1).

Bottom Line: The D-glucose levels were significantly higher in A12HM group.Maltase D expression level was significantly higher in males reared with sucrose.Body weights of the males reared with D-glucose and sucrose were significantly higher than those of the males reared with yeast extract.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Insect Ecology, College of Natural Resources and the Environment, South China Agricultural University, Guangzhou 510640, China.

ABSTRACT
Reproduction is an important life process in insects; however, few studies have attempted to demonstrate the association between reproductive activity and energy metabolism. To address this problem, we focused on the reproductive changes in Bactrocera dorsalis males. We analyzed B. dorsalis male gene expression profiles during mating (DM), 3 h after mating (A3HM) and 12 h after mating (A12HM). Gene annotation and pathway analyses of differentially expressed genes show that galactose metabolism and the starch and sucrose metabolism pathway activities were significantly higher in A12HM group. Moreover, the maltase D gene was the most strongly up-regulated gene. The D-glucose levels were significantly higher in A12HM group. Maltase D expression level was significantly higher in males reared with sucrose. Body weights of the males reared with D-glucose and sucrose were significantly higher than those of the males reared with yeast extract. We observed more mated males from the groups fed sucrose and D-glucose than from those fed yeast extract. The D-glucose levels in individual males were highest at 18:00 h, when flies exhibit the most active mating behavior. This study shows that the maltase D gene and D-glucose are the critical gene and substrate, respectively, in male B. dorsalis mating process.

Show MeSH