Limits...
Pancreas-targeted NIR fluorophores for dual-channel image-guided abdominal surgery.

Wada H, Hyun H, Vargas C, Gravier J, Park G, Gioux S, Frangioni JV, Henary M, Choi HS - Theranostics (2015)

Bottom Line: Three different 800 nm NIR fluorophores were employed for dual-channel FLARE™ imaging in pigs: 2 μmol of ZW800-1 for vessels and kidney, 1 μmol of ZW800-3C for lymph nodes, and 2 μmol of ESNF31 for adrenal glands.In pigs, T700-F produced an SBR≥2 against muscle, spleen, and lymph nodes for up to 8 h after a single intravenous injection.Pancreas-targeted NIR fluorophores combined with the FLARE dual-channel imaging system enable the real-time intraoperative pancreas imaging which helps surgeons perform safer and more curative abdominal surgeries.

View Article: PubMed Central - PubMed

Affiliation: 1. Division of Hematology/Oncology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215. ; 2. Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan.

ABSTRACT

Objective: Pancreas-related complications are some of the most serious ones in abdominal surgery. The goal of this study was to develop and validate novel near-infrared (NIR) fluorophores that would enable real-time pancreas imaging to avoid the intraoperative pancreatic injury.

Design: After initial screening of a large NIR fluorophore library, the performance of 3 selected pancreas-targeted 700 nm NIR fluorophores, T700-H, T700-F, and MB, were quantified in mice, rats, and pigs. Dose ranging using 25 and 100 nmol, and 2.5 µmol of T700-F, and its imaging kinetics over a 4 h period were tested in each species. Three different 800 nm NIR fluorophores were employed for dual-channel FLARE™ imaging in pigs: 2 μmol of ZW800-1 for vessels and kidney, 1 μmol of ZW800-3C for lymph nodes, and 2 μmol of ESNF31 for adrenal glands.

Results: T700-F demonstrated the highest signal to background ratio (SBR), with peak SBR at 4 h postinjection in mice. In pigs, T700-F produced an SBR≥2 against muscle, spleen, and lymph nodes for up to 8 h after a single intravenous injection. The combination of T700-F with each 800 nm NIR fluorophore provided simultaneous dual-channel intraoperative imaging of pancreas with surrounding organs in real time.

Conclusion: Pancreas-targeted NIR fluorophores combined with the FLARE dual-channel imaging system enable the real-time intraoperative pancreas imaging which helps surgeons perform safer and more curative abdominal surgeries.

Show MeSH

Related in: MedlinePlus

Simultaneous Dual Channel Imaging of Pancreas and Surrounding Tissues and Organs: 2.5 µmol of T700-F was injected intravenously for pancreas imaging 4 h prior to: 1st row: NIR angiography with 2 µmol intravenous injection of ZW800-1 immediately prior to imaging. 2nd row: Kidney imaging with 2 µmol intravenous injection of ZW800-1 30 min prior to imaging. 3rd row: Pan lymph node imaging with 1 µmol intravenous injection of ZW800-3C 4 h prior to imaging. 4th row: Adrenal gland imaging with 2 µmol intravenous injection of ESNF31 30 min prior to imaging (n = 3 pigs). Shown are color image, 700 nm NIR fluorescence, 800 nm NIR fluorescence, and a merged image of the three. For the merged image, FLARE™ channel #1 (700 nm) is pseudo-colored in red and channel #2 (800 nm) in green. Abbreviations used are: AG, adrenal gland; CHA, common hepatic artery; Du, duodenum; In, intestine; Ki, kidney; Li, liver; LGA, left gastric artery; Pa, pancreas; Sp, spleen; St, stomach; SPA, splenic artery. Arrowheads indicate tiny branch from artery (1st row), kidney (2nd row), pan lymph nodes (3rd row), or adrenal gland (4th row). Scale bars = 1 cm. All NIR fluorescence images have identical exposure times and normalizations.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4265744&req=5

Figure 5: Simultaneous Dual Channel Imaging of Pancreas and Surrounding Tissues and Organs: 2.5 µmol of T700-F was injected intravenously for pancreas imaging 4 h prior to: 1st row: NIR angiography with 2 µmol intravenous injection of ZW800-1 immediately prior to imaging. 2nd row: Kidney imaging with 2 µmol intravenous injection of ZW800-1 30 min prior to imaging. 3rd row: Pan lymph node imaging with 1 µmol intravenous injection of ZW800-3C 4 h prior to imaging. 4th row: Adrenal gland imaging with 2 µmol intravenous injection of ESNF31 30 min prior to imaging (n = 3 pigs). Shown are color image, 700 nm NIR fluorescence, 800 nm NIR fluorescence, and a merged image of the three. For the merged image, FLARE™ channel #1 (700 nm) is pseudo-colored in red and channel #2 (800 nm) in green. Abbreviations used are: AG, adrenal gland; CHA, common hepatic artery; Du, duodenum; In, intestine; Ki, kidney; Li, liver; LGA, left gastric artery; Pa, pancreas; Sp, spleen; St, stomach; SPA, splenic artery. Arrowheads indicate tiny branch from artery (1st row), kidney (2nd row), pan lymph nodes (3rd row), or adrenal gland (4th row). Scale bars = 1 cm. All NIR fluorescence images have identical exposure times and normalizations.

Mentions: To identify blood vessels, ZW800-1 was injected intravenously into pig 4 h after injection of T700-F. As shown in Figure 5, we could visualize not only important arteries in gastrointestinal surgery such as the common hepatic artery, left gastric artery, and splenic artery, but also extremely small vessel branches from the common hepatic artery.


Pancreas-targeted NIR fluorophores for dual-channel image-guided abdominal surgery.

Wada H, Hyun H, Vargas C, Gravier J, Park G, Gioux S, Frangioni JV, Henary M, Choi HS - Theranostics (2015)

Simultaneous Dual Channel Imaging of Pancreas and Surrounding Tissues and Organs: 2.5 µmol of T700-F was injected intravenously for pancreas imaging 4 h prior to: 1st row: NIR angiography with 2 µmol intravenous injection of ZW800-1 immediately prior to imaging. 2nd row: Kidney imaging with 2 µmol intravenous injection of ZW800-1 30 min prior to imaging. 3rd row: Pan lymph node imaging with 1 µmol intravenous injection of ZW800-3C 4 h prior to imaging. 4th row: Adrenal gland imaging with 2 µmol intravenous injection of ESNF31 30 min prior to imaging (n = 3 pigs). Shown are color image, 700 nm NIR fluorescence, 800 nm NIR fluorescence, and a merged image of the three. For the merged image, FLARE™ channel #1 (700 nm) is pseudo-colored in red and channel #2 (800 nm) in green. Abbreviations used are: AG, adrenal gland; CHA, common hepatic artery; Du, duodenum; In, intestine; Ki, kidney; Li, liver; LGA, left gastric artery; Pa, pancreas; Sp, spleen; St, stomach; SPA, splenic artery. Arrowheads indicate tiny branch from artery (1st row), kidney (2nd row), pan lymph nodes (3rd row), or adrenal gland (4th row). Scale bars = 1 cm. All NIR fluorescence images have identical exposure times and normalizations.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4265744&req=5

Figure 5: Simultaneous Dual Channel Imaging of Pancreas and Surrounding Tissues and Organs: 2.5 µmol of T700-F was injected intravenously for pancreas imaging 4 h prior to: 1st row: NIR angiography with 2 µmol intravenous injection of ZW800-1 immediately prior to imaging. 2nd row: Kidney imaging with 2 µmol intravenous injection of ZW800-1 30 min prior to imaging. 3rd row: Pan lymph node imaging with 1 µmol intravenous injection of ZW800-3C 4 h prior to imaging. 4th row: Adrenal gland imaging with 2 µmol intravenous injection of ESNF31 30 min prior to imaging (n = 3 pigs). Shown are color image, 700 nm NIR fluorescence, 800 nm NIR fluorescence, and a merged image of the three. For the merged image, FLARE™ channel #1 (700 nm) is pseudo-colored in red and channel #2 (800 nm) in green. Abbreviations used are: AG, adrenal gland; CHA, common hepatic artery; Du, duodenum; In, intestine; Ki, kidney; Li, liver; LGA, left gastric artery; Pa, pancreas; Sp, spleen; St, stomach; SPA, splenic artery. Arrowheads indicate tiny branch from artery (1st row), kidney (2nd row), pan lymph nodes (3rd row), or adrenal gland (4th row). Scale bars = 1 cm. All NIR fluorescence images have identical exposure times and normalizations.
Mentions: To identify blood vessels, ZW800-1 was injected intravenously into pig 4 h after injection of T700-F. As shown in Figure 5, we could visualize not only important arteries in gastrointestinal surgery such as the common hepatic artery, left gastric artery, and splenic artery, but also extremely small vessel branches from the common hepatic artery.

Bottom Line: Three different 800 nm NIR fluorophores were employed for dual-channel FLARE™ imaging in pigs: 2 μmol of ZW800-1 for vessels and kidney, 1 μmol of ZW800-3C for lymph nodes, and 2 μmol of ESNF31 for adrenal glands.In pigs, T700-F produced an SBR≥2 against muscle, spleen, and lymph nodes for up to 8 h after a single intravenous injection.Pancreas-targeted NIR fluorophores combined with the FLARE dual-channel imaging system enable the real-time intraoperative pancreas imaging which helps surgeons perform safer and more curative abdominal surgeries.

View Article: PubMed Central - PubMed

Affiliation: 1. Division of Hematology/Oncology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215. ; 2. Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan.

ABSTRACT

Objective: Pancreas-related complications are some of the most serious ones in abdominal surgery. The goal of this study was to develop and validate novel near-infrared (NIR) fluorophores that would enable real-time pancreas imaging to avoid the intraoperative pancreatic injury.

Design: After initial screening of a large NIR fluorophore library, the performance of 3 selected pancreas-targeted 700 nm NIR fluorophores, T700-H, T700-F, and MB, were quantified in mice, rats, and pigs. Dose ranging using 25 and 100 nmol, and 2.5 µmol of T700-F, and its imaging kinetics over a 4 h period were tested in each species. Three different 800 nm NIR fluorophores were employed for dual-channel FLARE™ imaging in pigs: 2 μmol of ZW800-1 for vessels and kidney, 1 μmol of ZW800-3C for lymph nodes, and 2 μmol of ESNF31 for adrenal glands.

Results: T700-F demonstrated the highest signal to background ratio (SBR), with peak SBR at 4 h postinjection in mice. In pigs, T700-F produced an SBR≥2 against muscle, spleen, and lymph nodes for up to 8 h after a single intravenous injection. The combination of T700-F with each 800 nm NIR fluorophore provided simultaneous dual-channel intraoperative imaging of pancreas with surrounding organs in real time.

Conclusion: Pancreas-targeted NIR fluorophores combined with the FLARE dual-channel imaging system enable the real-time intraoperative pancreas imaging which helps surgeons perform safer and more curative abdominal surgeries.

Show MeSH
Related in: MedlinePlus