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Dietary blueberry and bifidobacteria attenuate nonalcoholic fatty liver disease in rats by affecting SIRT1-mediated signaling pathway.

Ren T, Huang C, Cheng M - Oxid Med Cell Longev (2014)

Bottom Line: BJB group ameliorated NAFLD, which was better than BJ group (P < 0.05).Inversely, the levels of AST and ALT, TG, TC, LDL-c, and MDA were decreased from CG to BJB group (P < 0.05).Blueberry juice and bifidobacteria improve NAFLD by activating SIRTI-mediating signaling pathway.

View Article: PubMed Central - PubMed

Affiliation: Biochemistry Department, Affiliated Hospital of Guiyang Medical College, Guiyang, Guizhou 550004, China.

ABSTRACT
NAFLD model rats were established and divided into NAFLD model (MG group), SIRT1 RNAi (SI group), blueberry juice (BJ group), blueberry juice + bifidobacteria (BJB group), blueberry juice + SIRT1 RNAi (BJSI group), and blueberry juice + bifidobacteria + SIRT1 RNAi groups (BJBSI group). A group with normal rats was a control group (CG). BJB group ameliorated NAFLD, which was better than BJ group (P < 0.05). The lipid accumulation was lower in CG, BJ, and BJB groups than that in MG, SI, BJSI, and BJBSI groups (P < 0.05). The levels of SIRT1 and PPAR-α were higher in CG, BJ, and BJB groups than those in MG, SI, BJSI, and BJBSI groups (P < 0.05). The levels of SREBP-1c were lower in CG, BJ, and BJB groups than those in MG, SI, BJSI, and BJBSI groups (P < 0.05). The biochemical indexes SOD, GSH, and HDL-c were improved from CG to BJB group (P < 0.05). Inversely, the levels of AST and ALT, TG, TC, LDL-c, and MDA were decreased from CG to BJB group (P < 0.05). These changes enhance antioxidative capability and biochemical index of rats. Blueberry juice and bifidobacteria improve NAFLD by activating SIRTI-mediating signaling pathway.

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Immunohistochemistry (IHC) analysis for the effect of blueberry juice and bifidobacteria on SREBP-1c expression in NAFLD rat livers (200x). CG: in control group, SREBP-1c was lowly expressed with light brown color. MG: in NAFLD model group, SREBP-1c was highly expressed with dark brown color. SI: in NAFLD model group with SIRT1 RNAi, SREBP-1c was highly expressed with dark brown color. BJ: in blueberry juice group, SREBP-1c was lowly expressed with light brown color. BJSI: in blueberry juice and SIRT1 RNAi group, the expression of SREBP-1c was significantly enhanced with dark brown color. BJB: in blueberry juice and bifidobacteria group, SREBP-1c was lowly expressed with light brown color. BJBSI: in blueberry juice and bifidobacteria and SIRT1 RNAi group, the expression of SREBP-1c was significantly enhanced with dark brown color.
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fig5: Immunohistochemistry (IHC) analysis for the effect of blueberry juice and bifidobacteria on SREBP-1c expression in NAFLD rat livers (200x). CG: in control group, SREBP-1c was lowly expressed with light brown color. MG: in NAFLD model group, SREBP-1c was highly expressed with dark brown color. SI: in NAFLD model group with SIRT1 RNAi, SREBP-1c was highly expressed with dark brown color. BJ: in blueberry juice group, SREBP-1c was lowly expressed with light brown color. BJSI: in blueberry juice and SIRT1 RNAi group, the expression of SREBP-1c was significantly enhanced with dark brown color. BJB: in blueberry juice and bifidobacteria group, SREBP-1c was lowly expressed with light brown color. BJBSI: in blueberry juice and bifidobacteria and SIRT1 RNAi group, the expression of SREBP-1c was significantly enhanced with dark brown color.

Mentions: Immunohistochemistry staining indicated that SIRT1 was expressed in nucleus (Figure 4). SIRT1-positive staining was restricted to hepatic cells in control, BJ, and BJB groups, and a significantly stronger immune staining was noted in these groups. In MG, SI, BJSI, and BJBSI groups, SIRT1 was weaker immune staining with very light brown color. Compared with SIRT1, SREBP-1c was expressed in cytoplasm (Figure 5). SREBP-1c-negative staining was restricted to hepatic cells in CG, BJ, and BJB groups, and a significantly weaker immune staining was noted in these groups. In MG, SI, BJSI, and BJBSI groups, SREBP-1c was stronger immune staining with very light brown color. Just like SREBP-1c, PPAR-α was also expressed in cytoplasm (Figure 6). In contrast, PPAR-α-positive staining was restricted to hepatic cells in CG, BJ, and BJB groups, and a significantly stronger immune staining was noted in these groups (Figure 6). In MG, SI, BJSI, and BJBSI groups, PPAR-α was weaker immune staining with very light brown color (Figure 6). All the results suggested that SIRT1 can upregulate the expression of PPAR-α and downregulate the level of SREBP-1c.


Dietary blueberry and bifidobacteria attenuate nonalcoholic fatty liver disease in rats by affecting SIRT1-mediated signaling pathway.

Ren T, Huang C, Cheng M - Oxid Med Cell Longev (2014)

Immunohistochemistry (IHC) analysis for the effect of blueberry juice and bifidobacteria on SREBP-1c expression in NAFLD rat livers (200x). CG: in control group, SREBP-1c was lowly expressed with light brown color. MG: in NAFLD model group, SREBP-1c was highly expressed with dark brown color. SI: in NAFLD model group with SIRT1 RNAi, SREBP-1c was highly expressed with dark brown color. BJ: in blueberry juice group, SREBP-1c was lowly expressed with light brown color. BJSI: in blueberry juice and SIRT1 RNAi group, the expression of SREBP-1c was significantly enhanced with dark brown color. BJB: in blueberry juice and bifidobacteria group, SREBP-1c was lowly expressed with light brown color. BJBSI: in blueberry juice and bifidobacteria and SIRT1 RNAi group, the expression of SREBP-1c was significantly enhanced with dark brown color.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4265704&req=5

fig5: Immunohistochemistry (IHC) analysis for the effect of blueberry juice and bifidobacteria on SREBP-1c expression in NAFLD rat livers (200x). CG: in control group, SREBP-1c was lowly expressed with light brown color. MG: in NAFLD model group, SREBP-1c was highly expressed with dark brown color. SI: in NAFLD model group with SIRT1 RNAi, SREBP-1c was highly expressed with dark brown color. BJ: in blueberry juice group, SREBP-1c was lowly expressed with light brown color. BJSI: in blueberry juice and SIRT1 RNAi group, the expression of SREBP-1c was significantly enhanced with dark brown color. BJB: in blueberry juice and bifidobacteria group, SREBP-1c was lowly expressed with light brown color. BJBSI: in blueberry juice and bifidobacteria and SIRT1 RNAi group, the expression of SREBP-1c was significantly enhanced with dark brown color.
Mentions: Immunohistochemistry staining indicated that SIRT1 was expressed in nucleus (Figure 4). SIRT1-positive staining was restricted to hepatic cells in control, BJ, and BJB groups, and a significantly stronger immune staining was noted in these groups. In MG, SI, BJSI, and BJBSI groups, SIRT1 was weaker immune staining with very light brown color. Compared with SIRT1, SREBP-1c was expressed in cytoplasm (Figure 5). SREBP-1c-negative staining was restricted to hepatic cells in CG, BJ, and BJB groups, and a significantly weaker immune staining was noted in these groups. In MG, SI, BJSI, and BJBSI groups, SREBP-1c was stronger immune staining with very light brown color. Just like SREBP-1c, PPAR-α was also expressed in cytoplasm (Figure 6). In contrast, PPAR-α-positive staining was restricted to hepatic cells in CG, BJ, and BJB groups, and a significantly stronger immune staining was noted in these groups (Figure 6). In MG, SI, BJSI, and BJBSI groups, PPAR-α was weaker immune staining with very light brown color (Figure 6). All the results suggested that SIRT1 can upregulate the expression of PPAR-α and downregulate the level of SREBP-1c.

Bottom Line: BJB group ameliorated NAFLD, which was better than BJ group (P < 0.05).Inversely, the levels of AST and ALT, TG, TC, LDL-c, and MDA were decreased from CG to BJB group (P < 0.05).Blueberry juice and bifidobacteria improve NAFLD by activating SIRTI-mediating signaling pathway.

View Article: PubMed Central - PubMed

Affiliation: Biochemistry Department, Affiliated Hospital of Guiyang Medical College, Guiyang, Guizhou 550004, China.

ABSTRACT
NAFLD model rats were established and divided into NAFLD model (MG group), SIRT1 RNAi (SI group), blueberry juice (BJ group), blueberry juice + bifidobacteria (BJB group), blueberry juice + SIRT1 RNAi (BJSI group), and blueberry juice + bifidobacteria + SIRT1 RNAi groups (BJBSI group). A group with normal rats was a control group (CG). BJB group ameliorated NAFLD, which was better than BJ group (P < 0.05). The lipid accumulation was lower in CG, BJ, and BJB groups than that in MG, SI, BJSI, and BJBSI groups (P < 0.05). The levels of SIRT1 and PPAR-α were higher in CG, BJ, and BJB groups than those in MG, SI, BJSI, and BJBSI groups (P < 0.05). The levels of SREBP-1c were lower in CG, BJ, and BJB groups than those in MG, SI, BJSI, and BJBSI groups (P < 0.05). The biochemical indexes SOD, GSH, and HDL-c were improved from CG to BJB group (P < 0.05). Inversely, the levels of AST and ALT, TG, TC, LDL-c, and MDA were decreased from CG to BJB group (P < 0.05). These changes enhance antioxidative capability and biochemical index of rats. Blueberry juice and bifidobacteria improve NAFLD by activating SIRTI-mediating signaling pathway.

Show MeSH
Related in: MedlinePlus