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Clinical significance of IL-23 regulating IL-17A and/or IL-17F positive Th17 cells in chronic periodontitis.

Luo Z, Wang H, Wu Y, Sun Z, Wu Y - Mediators Inflamm. (2014)

Bottom Line: Additionally, correlation coefficient analysis showed significant correlation between IL-17A(+)IL-17F(-) Th17 cell and attachment loss or probing depth (P < 0.05).This study indicates that both the IL-17A(+)IL-17F(-) and IL-17A(-)IL-17F(+) Th17 cells may be involved in pathogenesis of periodontitis.The role of these Th17 cells in the disease pathogenesis needs to be further investigated.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontics, School of Stomatology, Capital Medical University, Beijing 100050, China ; State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University, Chengdu 610041, China.

ABSTRACT

Objective: To investigate the expression level and clinical significance of (IL-17A(+) and/or IL-17F(+)) Th17 cells under IL-23 regulation in patients of chronic periodontitis (CP) and healthy controls (HC).

Materials and methods: The whole peripheral blood samples were collected from 30 CP patients and 25 healthy controls. Flow cytometry was used to test the (IL-17A(+) and/or IL-17F(+)) Th17 expression level. Recombinant human IL-23 (rhIL-23) was used to detect Th17 differentiation and expansion. Correlation coefficient analysis between Th17 expression level and clinical parameters was analyzed by SPSS software.

Results: Flow cytometry results showed that IL-17A(+)IL-17F(-) and IL-17A(-)IL-17F(+) Th17 were both increased in CP group than in HC group (P < 0.01), while, under recombinant human IL-23 (rhIL-23) stimulation, the number of IL-17A(+)IL-17F(-) Th17 cells was significantly increased in both CP and HC groups (P < 0.01). Interestingly, IL-17A(-)IL-17F(+) Th17 cells were only increased in CP group after rhIL-23 stimulation. Additionally, correlation coefficient analysis showed significant correlation between IL-17A(+)IL-17F(-) Th17 cell and attachment loss or probing depth (P < 0.05).

Conclusions: This study indicates that both the IL-17A(+)IL-17F(-) and IL-17A(-)IL-17F(+) Th17 cells may be involved in pathogenesis of periodontitis. The role of these Th17 cells in the disease pathogenesis needs to be further investigated.

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Related in: MedlinePlus

Purity verification of separated CD4+ T cells by flow cytometry. (a) showed the isotype control performed by Simultest IgG2a/IgG1. (b) represents double staining by anti-human CD3 and CD4 antibody. (c) represents anti-human antibody CD4 staining. These results showed that the purity of CD4+ from PBMC can reach a percent of more than 95%.
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fig1: Purity verification of separated CD4+ T cells by flow cytometry. (a) showed the isotype control performed by Simultest IgG2a/IgG1. (b) represents double staining by anti-human CD3 and CD4 antibody. (c) represents anti-human antibody CD4 staining. These results showed that the purity of CD4+ from PBMC can reach a percent of more than 95%.

Mentions: Separated CD4+ T cells were validated by anti-human CD3 and CD4 flow cytometry antibody to test CD4 cell purity. The results showed that the purity of CD4+ T from PBMC can reach a percent of more than 95% (Figure 1). Flow cytometry of the sample staining with IL-17A and IL-17F antibody was demonstrated in Figure 2 and further data analysis was shown in Figure 3. Flow cytometry analysis showed significantly elevated levels of IL-17A+IL-17F− Th17 cell (Figure 3(a), P < 0.01) and IL-17A−IL-17F+ Th17 cell in CP group compared with HC group (Figure 3(a), P < 0.01). However, it did not demonstrate statistically significant difference between CP group and HC group with the IL-17A+IL-17F+ double positive Th17 cell (Figure 3(a), P > 0.05).


Clinical significance of IL-23 regulating IL-17A and/or IL-17F positive Th17 cells in chronic periodontitis.

Luo Z, Wang H, Wu Y, Sun Z, Wu Y - Mediators Inflamm. (2014)

Purity verification of separated CD4+ T cells by flow cytometry. (a) showed the isotype control performed by Simultest IgG2a/IgG1. (b) represents double staining by anti-human CD3 and CD4 antibody. (c) represents anti-human antibody CD4 staining. These results showed that the purity of CD4+ from PBMC can reach a percent of more than 95%.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4265697&req=5

fig1: Purity verification of separated CD4+ T cells by flow cytometry. (a) showed the isotype control performed by Simultest IgG2a/IgG1. (b) represents double staining by anti-human CD3 and CD4 antibody. (c) represents anti-human antibody CD4 staining. These results showed that the purity of CD4+ from PBMC can reach a percent of more than 95%.
Mentions: Separated CD4+ T cells were validated by anti-human CD3 and CD4 flow cytometry antibody to test CD4 cell purity. The results showed that the purity of CD4+ T from PBMC can reach a percent of more than 95% (Figure 1). Flow cytometry of the sample staining with IL-17A and IL-17F antibody was demonstrated in Figure 2 and further data analysis was shown in Figure 3. Flow cytometry analysis showed significantly elevated levels of IL-17A+IL-17F− Th17 cell (Figure 3(a), P < 0.01) and IL-17A−IL-17F+ Th17 cell in CP group compared with HC group (Figure 3(a), P < 0.01). However, it did not demonstrate statistically significant difference between CP group and HC group with the IL-17A+IL-17F+ double positive Th17 cell (Figure 3(a), P > 0.05).

Bottom Line: Additionally, correlation coefficient analysis showed significant correlation between IL-17A(+)IL-17F(-) Th17 cell and attachment loss or probing depth (P < 0.05).This study indicates that both the IL-17A(+)IL-17F(-) and IL-17A(-)IL-17F(+) Th17 cells may be involved in pathogenesis of periodontitis.The role of these Th17 cells in the disease pathogenesis needs to be further investigated.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontics, School of Stomatology, Capital Medical University, Beijing 100050, China ; State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University, Chengdu 610041, China.

ABSTRACT

Objective: To investigate the expression level and clinical significance of (IL-17A(+) and/or IL-17F(+)) Th17 cells under IL-23 regulation in patients of chronic periodontitis (CP) and healthy controls (HC).

Materials and methods: The whole peripheral blood samples were collected from 30 CP patients and 25 healthy controls. Flow cytometry was used to test the (IL-17A(+) and/or IL-17F(+)) Th17 expression level. Recombinant human IL-23 (rhIL-23) was used to detect Th17 differentiation and expansion. Correlation coefficient analysis between Th17 expression level and clinical parameters was analyzed by SPSS software.

Results: Flow cytometry results showed that IL-17A(+)IL-17F(-) and IL-17A(-)IL-17F(+) Th17 were both increased in CP group than in HC group (P < 0.01), while, under recombinant human IL-23 (rhIL-23) stimulation, the number of IL-17A(+)IL-17F(-) Th17 cells was significantly increased in both CP and HC groups (P < 0.01). Interestingly, IL-17A(-)IL-17F(+) Th17 cells were only increased in CP group after rhIL-23 stimulation. Additionally, correlation coefficient analysis showed significant correlation between IL-17A(+)IL-17F(-) Th17 cell and attachment loss or probing depth (P < 0.05).

Conclusions: This study indicates that both the IL-17A(+)IL-17F(-) and IL-17A(-)IL-17F(+) Th17 cells may be involved in pathogenesis of periodontitis. The role of these Th17 cells in the disease pathogenesis needs to be further investigated.

Show MeSH
Related in: MedlinePlus