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Transcriptome Profiling of Wheat Seedlings following Treatment with Ultrahigh Diluted Arsenic Trioxide.

Marotti I, Betti L, Bregola V, Bosi S, Trebbi G, Borghini G, Nani D, Dinelli G - Evid Based Complement Alternat Med (2014)

Bottom Line: The objective of the present study was to test the hypothesis whether 7-day-old wheat seedlings, grown from seeds either poisoned with a sublethal dose of As2O3 or unpoisoned, showed different significant gene expression profiles after the application of ultrahigh diluted As2O3 (beyond Avogadro's limit) compared to water (control).The results provided evidence for a strong gene modulating effect of ultrahigh diluted As2O3 in seedlings grown from poisoned seeds: a massive reduction of gene expression levels to values comparable to those of the control group was observed for several functional classes of genes.A plausible hypothesis is that ultrahigh diluted As2O3 treatment induced a reequilibration of those genes that were upregulated during the oxidative stress by bringing the expression levels closer to the basal levels normally occurring in the control plants.

View Article: PubMed Central - PubMed

Affiliation: Department of Agricultural Sciences, University of Bologna, Viale Fanin 44, 40127 Bologna, Italy.

ABSTRACT
Plant systems are useful research tools to address basic questions in homeopathy as they make it possible to overcome some of the drawbacks encountered in clinical trials (placebo effect, ethical issues, duration of the experiment, and high costs). The objective of the present study was to test the hypothesis whether 7-day-old wheat seedlings, grown from seeds either poisoned with a sublethal dose of As2O3 or unpoisoned, showed different significant gene expression profiles after the application of ultrahigh diluted As2O3 (beyond Avogadro's limit) compared to water (control). The results provided evidence for a strong gene modulating effect of ultrahigh diluted As2O3 in seedlings grown from poisoned seeds: a massive reduction of gene expression levels to values comparable to those of the control group was observed for several functional classes of genes. A plausible hypothesis is that ultrahigh diluted As2O3 treatment induced a reequilibration of those genes that were upregulated during the oxidative stress by bringing the expression levels closer to the basal levels normally occurring in the control plants.

No MeSH data available.


Effect of ultrahigh diluted arsenic on wheat coleoptile gene expression (P + T versus C + 0) grown from poisoned seeds as compared to unpoisoned seeds. The complete dataset used for MapMan analysis and related details are given in Table S2.
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Related In: Results  -  Collection


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fig6: Effect of ultrahigh diluted arsenic on wheat coleoptile gene expression (P + T versus C + 0) grown from poisoned seeds as compared to unpoisoned seeds. The complete dataset used for MapMan analysis and related details are given in Table S2.

Mentions: To this aim a custom MapMan image pathway was generated and used for visualizing probe sets induced and/or repressed in four pairwise comparisons: treated control (C + T) versus control (C + 0) (Figure 3), poisoned (P + 0) versus control (C + 0) (Figure 4), poisoned-treated (P + T) versus poisoned (P + 0) (Figure 5), and poisoned-treated (P + T) versus control (C + 0) (Figure 6).


Transcriptome Profiling of Wheat Seedlings following Treatment with Ultrahigh Diluted Arsenic Trioxide.

Marotti I, Betti L, Bregola V, Bosi S, Trebbi G, Borghini G, Nani D, Dinelli G - Evid Based Complement Alternat Med (2014)

Effect of ultrahigh diluted arsenic on wheat coleoptile gene expression (P + T versus C + 0) grown from poisoned seeds as compared to unpoisoned seeds. The complete dataset used for MapMan analysis and related details are given in Table S2.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4265686&req=5

fig6: Effect of ultrahigh diluted arsenic on wheat coleoptile gene expression (P + T versus C + 0) grown from poisoned seeds as compared to unpoisoned seeds. The complete dataset used for MapMan analysis and related details are given in Table S2.
Mentions: To this aim a custom MapMan image pathway was generated and used for visualizing probe sets induced and/or repressed in four pairwise comparisons: treated control (C + T) versus control (C + 0) (Figure 3), poisoned (P + 0) versus control (C + 0) (Figure 4), poisoned-treated (P + T) versus poisoned (P + 0) (Figure 5), and poisoned-treated (P + T) versus control (C + 0) (Figure 6).

Bottom Line: The objective of the present study was to test the hypothesis whether 7-day-old wheat seedlings, grown from seeds either poisoned with a sublethal dose of As2O3 or unpoisoned, showed different significant gene expression profiles after the application of ultrahigh diluted As2O3 (beyond Avogadro's limit) compared to water (control).The results provided evidence for a strong gene modulating effect of ultrahigh diluted As2O3 in seedlings grown from poisoned seeds: a massive reduction of gene expression levels to values comparable to those of the control group was observed for several functional classes of genes.A plausible hypothesis is that ultrahigh diluted As2O3 treatment induced a reequilibration of those genes that were upregulated during the oxidative stress by bringing the expression levels closer to the basal levels normally occurring in the control plants.

View Article: PubMed Central - PubMed

Affiliation: Department of Agricultural Sciences, University of Bologna, Viale Fanin 44, 40127 Bologna, Italy.

ABSTRACT
Plant systems are useful research tools to address basic questions in homeopathy as they make it possible to overcome some of the drawbacks encountered in clinical trials (placebo effect, ethical issues, duration of the experiment, and high costs). The objective of the present study was to test the hypothesis whether 7-day-old wheat seedlings, grown from seeds either poisoned with a sublethal dose of As2O3 or unpoisoned, showed different significant gene expression profiles after the application of ultrahigh diluted As2O3 (beyond Avogadro's limit) compared to water (control). The results provided evidence for a strong gene modulating effect of ultrahigh diluted As2O3 in seedlings grown from poisoned seeds: a massive reduction of gene expression levels to values comparable to those of the control group was observed for several functional classes of genes. A plausible hypothesis is that ultrahigh diluted As2O3 treatment induced a reequilibration of those genes that were upregulated during the oxidative stress by bringing the expression levels closer to the basal levels normally occurring in the control plants.

No MeSH data available.