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Effect of Centella asiatica Leaf Extract on the Dietary Supplementation in Transgenic Drosophila Model of Parkinson's Disease.

Siddique YH, Naz F, Jyoti S, Fatima A, Khanam S - Parkinsons Dis (2014)

Bottom Line: The leaf extract was prepared in acetone and was subjected to GC-MS analysis.C. asiatica extract at final concentration of 0.25, 0.50, and 1.0 μL/mL was mixed with the diet and the flies were allowed feeding on it for 24 days.The exposure of extract to PD model flies results in a significant delay in the loss of climbing ability and activity pattern and reduced the oxidative stress (P < 0.05) in the brains of PD flies as compared to untreated PD flies.

View Article: PubMed Central - PubMed

Affiliation: Drosophila Transgenic Laboratory, Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh 202002, India.

ABSTRACT
The role of Centella asiatica L. leaf extract was studied on the transgenic Drosophila model flies expressing normal human alpha synuclein (h-αS) in the neurons. The leaf extract was prepared in acetone and was subjected to GC-MS analysis. C. asiatica extract at final concentration of 0.25, 0.50, and 1.0 μL/mL was mixed with the diet and the flies were allowed feeding on it for 24 days. The effect of extract was studied on the climbing ability, activity pattern, lipid peroxidation, protein carbonyl content, glutathione content, and glutathione-S-transferase activity in the brains of transgenic Drosophila. The exposure of extract to PD model flies results in a significant delay in the loss of climbing ability and activity pattern and reduced the oxidative stress (P < 0.05) in the brains of PD flies as compared to untreated PD flies. The results suggest that C. asiatica leaf extract is potent in reducing the PD symptoms in transgenic Drosophila model of Parkinson's disease.

No MeSH data available.


Related in: MedlinePlus

Effect of Centella asiatica extract (CAE) on glutathione-S-transferase (GST) content measured in the brains of transgenic Drosophila melanogaster after 24 days of the exposure to various treated groups (asignificant with respect to control, P-0.01; bsignificant with respect to PD model flies, P-0.05). L-Dopa = 10−3 M; control flies: UAS-alpha-syn; PD flies: elav-GAL4; UAS-alpha-syn.
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fig17: Effect of Centella asiatica extract (CAE) on glutathione-S-transferase (GST) content measured in the brains of transgenic Drosophila melanogaster after 24 days of the exposure to various treated groups (asignificant with respect to control, P-0.01; bsignificant with respect to PD model flies, P-0.05). L-Dopa = 10−3 M; control flies: UAS-alpha-syn; PD flies: elav-GAL4; UAS-alpha-syn.

Mentions: The results obtained for total glutathione (GSH) content are shown in (Figure 16). A dose-dependent increase in the GSH content was observed in the PD flies exposed to 0.25, 0.50, and 1 μL/mL of C. asiatica extract (Figure 16). The control flies exposed to 0.25, 0.50, and 1 μL/mL of C. asiatica extract also showed a dose-dependent increase in the GSH content as compared to untreated control flies (Figure 16). The results obtained for the estimation of glutathione-S-transferase (GST) activity are shown in (Figure 17). A significant increase in the activity of GST was observed in PD flies (Figure 17). The exposure of PD flies to 0.25, 0.50, and 1 μL/mL of C. asiatica extract showed a dose-dependent decrease in the GST activity (Figure 17). No increase in the GST activity was observed in control flies exposed to 0.25, 0.50,and 1 μL/mL of C. asiatica extract (Figure 17).


Effect of Centella asiatica Leaf Extract on the Dietary Supplementation in Transgenic Drosophila Model of Parkinson's Disease.

Siddique YH, Naz F, Jyoti S, Fatima A, Khanam S - Parkinsons Dis (2014)

Effect of Centella asiatica extract (CAE) on glutathione-S-transferase (GST) content measured in the brains of transgenic Drosophila melanogaster after 24 days of the exposure to various treated groups (asignificant with respect to control, P-0.01; bsignificant with respect to PD model flies, P-0.05). L-Dopa = 10−3 M; control flies: UAS-alpha-syn; PD flies: elav-GAL4; UAS-alpha-syn.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4265550&req=5

fig17: Effect of Centella asiatica extract (CAE) on glutathione-S-transferase (GST) content measured in the brains of transgenic Drosophila melanogaster after 24 days of the exposure to various treated groups (asignificant with respect to control, P-0.01; bsignificant with respect to PD model flies, P-0.05). L-Dopa = 10−3 M; control flies: UAS-alpha-syn; PD flies: elav-GAL4; UAS-alpha-syn.
Mentions: The results obtained for total glutathione (GSH) content are shown in (Figure 16). A dose-dependent increase in the GSH content was observed in the PD flies exposed to 0.25, 0.50, and 1 μL/mL of C. asiatica extract (Figure 16). The control flies exposed to 0.25, 0.50, and 1 μL/mL of C. asiatica extract also showed a dose-dependent increase in the GSH content as compared to untreated control flies (Figure 16). The results obtained for the estimation of glutathione-S-transferase (GST) activity are shown in (Figure 17). A significant increase in the activity of GST was observed in PD flies (Figure 17). The exposure of PD flies to 0.25, 0.50, and 1 μL/mL of C. asiatica extract showed a dose-dependent decrease in the GST activity (Figure 17). No increase in the GST activity was observed in control flies exposed to 0.25, 0.50,and 1 μL/mL of C. asiatica extract (Figure 17).

Bottom Line: The leaf extract was prepared in acetone and was subjected to GC-MS analysis.C. asiatica extract at final concentration of 0.25, 0.50, and 1.0 μL/mL was mixed with the diet and the flies were allowed feeding on it for 24 days.The exposure of extract to PD model flies results in a significant delay in the loss of climbing ability and activity pattern and reduced the oxidative stress (P < 0.05) in the brains of PD flies as compared to untreated PD flies.

View Article: PubMed Central - PubMed

Affiliation: Drosophila Transgenic Laboratory, Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh 202002, India.

ABSTRACT
The role of Centella asiatica L. leaf extract was studied on the transgenic Drosophila model flies expressing normal human alpha synuclein (h-αS) in the neurons. The leaf extract was prepared in acetone and was subjected to GC-MS analysis. C. asiatica extract at final concentration of 0.25, 0.50, and 1.0 μL/mL was mixed with the diet and the flies were allowed feeding on it for 24 days. The effect of extract was studied on the climbing ability, activity pattern, lipid peroxidation, protein carbonyl content, glutathione content, and glutathione-S-transferase activity in the brains of transgenic Drosophila. The exposure of extract to PD model flies results in a significant delay in the loss of climbing ability and activity pattern and reduced the oxidative stress (P < 0.05) in the brains of PD flies as compared to untreated PD flies. The results suggest that C. asiatica leaf extract is potent in reducing the PD symptoms in transgenic Drosophila model of Parkinson's disease.

No MeSH data available.


Related in: MedlinePlus